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Br J Cancer. May 1989; 59(5): 677–681.
PMCID: PMC2247211

Effect of insulin on weight loss and tumour growth in a cachexia model.

Abstract

A comparison has been made between the effects of daily insulin injection and a ketogenic diet on weight loss and tumour weight in an experimental model of cancer cachexia (MAC16). Weight loss associated with the MAC16 tumour was significantly reduced both by a ketogenic diet (80% MCT) and by daily insulin injections without an increase in either food or water consumption. Animals fed the 80% MCT diet had a significantly reduced tumour weight compared with controls fed a normal laboratory diet, while in animals administered 20 U insulin kg-1 day-1 the tumour weight was 50% greater than in saline infused controls. The stimulation of tumour growth by insulin was counteracted by the inclusion of 3-hydroxybutyrate in the drinking water without any alteration in the extent of weight loss. Depletion of both carcass fat and muscle dry weight in animals bearing the MAC16 tumour was reversed in animals administered either insulin or an 80% MCT diet. Animals bearing the MAC16 tumour had a reduced nitrogen balance compared with non-tumour-bearing controls, mainly due to excess urea excretion, and this was reversed towards control values in animals fed an 80% MCT diet, but not in animals administered insulin. These results suggest that a ketogenic diet is more effective than insulin administration in reversing the cachectic process and has the advantage of a concomitant reduction in tumour weight.

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Selected References

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  • Barnes D, Sato G. Methods for growth of cultured cells in serum-free medium. Anal Biochem. 1980 Mar 1;102(2):255–270. [PubMed]
  • Beck SA, Tisdale MJ. Production of lipolytic and proteolytic factors by a murine tumor-producing cachexia in the host. Cancer Res. 1987 Nov 15;47(22):5919–5923. [PubMed]
  • Bibby MC, Double JA, Ali SA, Fearon KC, Brennan RA, Tisdale MJ. Characterization of a transplantable adenocarcinoma of the mouse colon producing cachexia in recipient animals. J Natl Cancer Inst. 1987 Mar;78(3):539–546. [PubMed]
  • Björntorp P. Effect of ketone bodies on lipolysis in adipose tissue in vitro. J Lipid Res. 1966 Sep;7(5):621–626. [PubMed]
  • Chance WT, Muggia-Sullam M, Chen MH, Murphy RF, Fischer JE. Reversal of tumor-induced biochemical abnormalities by insulin treatment in rats. J Natl Cancer Inst. 1986 Aug;77(2):497–503. [PubMed]
  • Cobb MH, Rosen OM. The insulin receptor and tyrosine protein kinase activity. Biochim Biophys Acta. 1984;738(1-2):1–8. [PubMed]
  • Dewys WD, Begg C, Lavin PT, Band PR, Bennett JM, Bertino JR, Cohen MH, Douglass HO, Jr, Engstrom PF, Ezdinli EZ, et al. Prognostic effect of weight loss prior to chemotherapy in cancer patients. Eastern Cooperative Oncology Group. Am J Med. 1980 Oct;69(4):491–497. [PubMed]
  • Fearon KC, Borland W, Preston T, Tisdale MJ, Shenkin A, Calman KC. Cancer cachexia: influence of systemic ketosis on substrate levels and nitrogen metabolism. Am J Clin Nutr. 1988 Jan;47(1):42–48. [PubMed]
  • Fulks RM, Li JB, Goldberg AL. Effects of insulin, glucose, and amino acids on protein turnover in rat diaphragm. J Biol Chem. 1975 Jan 10;250(1):290–298. [PubMed]
  • Gold J. Cancer cachexia and gluconeogenesis. Ann N Y Acad Sci. 1974;230:103–110. [PubMed]
  • Heber D, Byerley LO, Chi J, Grosvenor M, Bergman RN, Coleman M, Chlebowski RT. Pathophysiology of malnutrition in the adult cancer patient. Cancer. 1986 Oct 15;58(8 Suppl):1867–1873. [PubMed]
  • Hissin PJ, Hilf R. Effects of insulin in vivo and in vitro on amino acid transport into cells from the R3230AC mammary adenocarcinoma and their relationship to tumor growth. Cancer Res. 1978 Nov;38(11 Pt 1):3646–3655. [PubMed]
  • Inculet RI, Peacock JL, Gorschboth CM, Norton JA. Gluconeogenesis in the tumor-influenced rat hepatocyte: importance of tumor burden, lactate, insulin, and glucagon. J Natl Cancer Inst. 1987 Nov;79(5):1039–1046. [PubMed]
  • Moley JF, Morrison SD, Norton JA. Insulin reversal of cancer cachexia in rats. Cancer Res. 1985 Oct;45(10):4925–4931. [PubMed]
  • Moley JF, Morrison SD, Gorschboth CM, Norton JA. Body composition changes in rats with experimental cancer cachexia: improvement with exogenous insulin. Cancer Res. 1988 May 15;48(10):2784–2787. [PubMed]
  • Rivlin RS, Shils ME, Sherlock P. Nutrition and cancer. Am J Med. 1983 Nov;75(5):843–854. [PubMed]
  • Sauer LA, Dauchy RT. Stimulation of tumor growth in adult rats in vivo during acute streptozotocin-induced diabetes. Cancer Res. 1987 Apr 1;47(7):1756–1761. [PubMed]
  • Schein PS, Kisner D, Haller D, Blecher M, Hamosh M. Cachexia of malignancy: potential role of insulin in nutritional management. Cancer. 1979 May;43(5 Suppl):2070–2076. [PubMed]
  • Tisdale MJ, Brennan RA, Fearon KC. Reduction of weight loss and tumour size in a cachexia model by a high fat diet. Br J Cancer. 1987 Jul;56(1):39–43. [PMC free article] [PubMed]

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