• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Aug 1978; 135(2): 402–407.
PMCID: PMC222396

Presence of glycerophospholipid: cholesterol acyltransferase and phospholipase in culture supernatant of Aeromonas hydrophila.

Abstract

Human erythrocyte membrane glycerophospholipids are deacylated by Aeromonas hydrophila 13-h culture supernatants, resulting in the production of cholesterol ester, free fatty acid, and water-soluble phosphates. This activity appears to be due to the actions of an acyltransferase (phosphatide:cholesterol acyltransferase, EC 2.3.1 group) and a phospholipase (phosphatide acyl-hydrolase). The enzyme activities are produced simultaneously in late exponential/early stationary phase, are precipitated together from the culture supernatant with 85% ammonium sulfate, and are eluted together near the void volume during gel filtration on Sepharose 6B. These results suggest that A. hydrophila produces a multienzyme complex with an unusual mode of action on membrane lipids. The complex is distinct from the hemolytic factor aerolysin, which is also produced by A. hydrophila.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (959K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BARTLETT GR. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed]
  • Bernheimer AW, Avigad LS. Partial characterization of aerolysin, a lytic exotoxin from Aeromonas hydrophila. Infect Immun. 1974 Jun;9(6):1016–1021. [PMC free article] [PubMed]
  • Bernheimer AW, Avigad LS, Avigad G. Interactions between aerolysin, erythrocytes, and erythrocyte membranes. Infect Immun. 1975 Jun;11(6):1312–1319. [PMC free article] [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Buckley JT, Hawthorne JN. Erythrocyte membrane polyphosphoinositide metabolism and the regulation of calcium binding. J Biol Chem. 1972 Nov 25;247(22):7218–7223. [PubMed]
  • DAWSON RM. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. [PMC free article] [PubMed]
  • Duncombe WG. The colorimetric micro-determination of long-chain fatty acids. Biochem J. 1963 Jul;88(1):7–10. [PMC free article] [PubMed]
  • Gatt S, Barenholz Y. Enzymes of complex lipid metabolism. Annu Rev Biochem. 1973;42(0):61–90. [PubMed]
  • Glenn AR. Production of extracellular proteins by bacteria. Annu Rev Microbiol. 1976;30:41–62. [PubMed]
  • Owens JJ. The egg yolk reaction produced by several species of bacteria. J Appl Bacteriol. 1974 Mar;37(1):137–148. [PubMed]
  • SPERRY WM, WEBB M. A revision of the Schoenheimer-Sperry method for cholesterol determination. J Biol Chem. 1950 Nov;187(1):97–106. [PubMed]
  • Torregrossa RE, Makula RA, Finnerty WR. Characterization of lysocardiolipin from Acinetobacter sp. HO1-N. J Bacteriol. 1977 Aug;131(2):486–492. [PMC free article] [PubMed]
  • Turner JD, Rouser G. Precise quantitative determination of human blood lipids by thin-layer and triethylaminoethylcellulose column chromatography. I. Erythrocyte lipids. Anal Biochem. 1970 Dec;38(2):423–436. [PubMed]
  • Wadström T, Ljungh A, Wretlind B. Enterotoxin, haemolysin and cytotoxic protein in Aeromonas hydrophila from human infections. Acta Pathol Microbiol Scand B. 1976 Apr;84(2):112–114. [PubMed]
  • Wretlind B, Hedén L, Wadström T. Formation of extracellular haemolysin by Aeromonas hydrophila in relation to protease and staphylolytic enzyme. J Gen Microbiol. 1973 Sep;78(1):57–65. [PubMed]
  • Wretlind B, Möllby R, Wadström T. Separation of two hemolysins from Aeromonas hydrophila by isoelectric focusing. Infect Immun. 1971 Oct;4(4):503–505. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...