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J Bacteriol. Apr 1978; 134(1): 157–166.
PMCID: PMC222230

Escherichia coli mutants deficient in deoxyuridine triphosphatase.


Mutants deficient in deoxyuridine triphosphatase (dUTPase) were identified by enzyme assays of randomly chosen heavily mutagenized clones. Five mutants of independent origin were obtained. One mutant produced a thermolabile enzyme, and it was presumed to have a mutation in the structural gene for dUTPase, designated dut. The most deficient mutant had the following associated phenotypes: less than 1% of parental dUTPase activity, prolonged generation time, increased sensitivity to 5'-fluorodeoxyuridine, increased rate of spontaneous mutation, increased rate of recombination (hyper-Rec), an inhibition of growth in the presence of 2 mM uracil, and a decreased ability to support the growth of phage P1 (but not T4 or lambda). This mutation also appeared to be incompatible with pyrE mutations. A revertant selected by its faster growth had regained dUTPase activity and lost its hyper-Rec phenotype. Many of the properties of the dut mutants are compatible with their presumed increased incorporation of uracil into DNA and the subsequent transient breakage of the DNA by excision repair.

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Selected References

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  • Bachmann BJ, Low KB, Taylor AL. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. [PMC free article] [PubMed]
  • Beck CF, Eisenhardt AR, Neuhard J. Deoxycytidine triphosphate deaminase of Salmonella typhimurium. Purification and characterization. J Biol Chem. 1975 Jan 25;250(2):609–616. [PubMed]
  • Beck CF, Neuhard J, Thomassen E. Thymidine-requiring mutants of Salmonella typhimurium that are defective in deoxyuridine 5'-phosphate synthesis. J Bacteriol. 1977 Jan;129(1):305–316. [PMC free article] [PubMed]
  • BECKWITH JR, PARDEE AB, AUSTRIAN R, JACOB F. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol. 1962 Dec;5:618–634. [PubMed]
  • Friedkin M. Thymidylate synthetase. Adv Enzymol Relat Areas Mol Biol. 1973;38:235–292. [PubMed]
  • Gallant J, Spottswood T. The recombinogenic effect of thymidylate starvation in Escherichia coli merodiploids. Genetics. 1965 Jul;52(1):107–118. [PMC free article] [PubMed]
  • Gates FT, 3rd, Linn S. Endonuclease V of Escherichia coli. J Biol Chem. 1977 Mar 10;252(5):1647–1653. [PubMed]
  • Glynn IM, Chappell JB. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. [PMC free article] [PubMed]
  • Greenberg GR. New dUTPase and dUDPase activites after infection of Escherichia coli by T2 bacteriophage. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1226–1232. [PMC free article] [PubMed]
  • GREENBERG GR, SOMERVILLE RL. Deoxyuridylate kinase activity and deoxyuridinetriphosphatase in Escherichia coli. Proc Natl Acad Sci U S A. 1962 Feb;48:247–257. [PMC free article] [PubMed]
  • Howard-Flanders P, Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. [PMC free article] [PubMed]
  • Howe MM. Prophage deletion mapping of bacteriophage Mu-1. Virology. 1973 Jul;54(1):93–101. [PubMed]
  • Kirtikar DM, Cathcart GR, Goldthwait DA. Endonuclease II, apurinic acid endonuclease, and exonuclease III. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4324–4328. [PMC free article] [PubMed]
  • Kleckner N, Chan RK, Tye BK, Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. [PubMed]
  • Konrad EB. Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications. J Bacteriol. 1977 Apr;130(1):167–172. [PMC free article] [PubMed]
  • Konrad EB, Lehman IR. Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2150–2154. [PMC free article] [PubMed]
  • Lindahl T, Ljungquist S, Siegert W, Nyberg B, Sperens B. DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli. J Biol Chem. 1977 May 25;252(10):3286–3294. [PubMed]
  • Ljungquist S. A new endonuclease from Escherichia coli acting at apurinic sites in DNA. J Biol Chem. 1977 May 10;252(9):2808–2814. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Isolation and characterization of a Bacillus subtilis mutant with a defective N-glycosidase activity for uracil-containing deoxyribonucleic acid. J Bacteriol. 1977 Aug;131(2):438–445. [PMC free article] [PubMed]
  • Marcus M, Halpern YS. Genetic analysis of the glutamate permease in Escherichia coli K-12. J Bacteriol. 1969 Mar;97(3):1118–1128. [PMC free article] [PubMed]
  • Milcarek C, Weiss B. Mutants of Escherichia coli with altered deoxyribonucleases. I. Isolation and characterization of mutants for exonuclease 3. J Mol Biol. 1972 Jul 21;68(2):303–318. [PubMed]
  • Nakayama H, Hanawalt P. Sedimentation analysis of deoxyribonucleic acid from thymine-starved Escherichia coli. J Bacteriol. 1975 Feb;121(2):537–547. [PMC free article] [PubMed]
  • O'Donovan GA, Edlin G, Fuchs JA, Neuhard J, Thomassen E. Deoxycytidine triphosphate deaminase: characterization of an Escherichia coli mutant deficient in the enzyme. J Bacteriol. 1971 Feb;105(2):666–672. [PMC free article] [PubMed]
  • Tye BK, Lehman IR. Excision repair of uracil incorporated in DNA as a result of a defect in dUTPase. J Mol Biol. 1977 Dec 5;117(2):293–306. [PubMed]
  • Tye BK, Nyman PO, Lehman IR, Hochhauser S, Weiss B. Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Proc Natl Acad Sci U S A. 1977 Jan;74(1):154–157. [PMC free article] [PubMed]
  • Verly WG, Rassart E. Purification of Escherichia coli endonuclease specific for apurinic sites in DNA. J Biol Chem. 1975 Oct 25;250(20):8214–8219. [PubMed]
  • Warner HR, Barnes JE. Evidence for a dual role for the bacteriophage T4-induced deoxycytidine triphosphate nucleotidohydrolase. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1233–1240. [PMC free article] [PubMed]
  • Weiss B. Endonuclease II of Escherichia coli is exonuclease III. J Biol Chem. 1976 Apr 10;251(7):1896–1901. [PubMed]
  • Weiss B, Milcarek C. Mass screening for mutants with altered DNases by microassay techniques. Methods Enzymol. 1974;29:180–193. [PubMed]
  • White BJ, Hochhauser SJ, Cintron NM, Weiss B. Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12. J Bacteriol. 1976 Jun;126(3):1082–1088. [PMC free article] [PubMed]
  • Witkin EM. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. [PMC free article] [PubMed]
  • Zieg J, Kushner SR. Analysis of genetic recombination between two partially deleted lactose operons of Escherichia coli K-12. J Bacteriol. 1977 Jul;131(1):123–132. [PMC free article] [PubMed]

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