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J Bacteriol. Feb 1978; 133(2): 708–716.
PMCID: PMC222079

Chemotaxis of Salmonella typhimurium to Amino Acids and Some Sugars

Thoyd Melton,†† Philip E. Hartman, John P. Stratis,††† Timothy L. Lee,†††† and Alan T. Davis†††††

Abstract

Patterns of chemotaxis by Salmonella typhimurium strain LT-2 to l-amino acids and to several sugars were quantitated by the Adler capillary procedure. Competition experiments indicated that LT-2 possesses three predominant receptors, or interacting sets of receptors, for amino acids. These were termed the aspartate, serine, and alanine classes, respectively. Studies with strains carrying point and deletion mutations affecting components of the phosphoenolpyruvate: glycose phosphotransferase system (PTS) made unlikely a role in primary reception of d-glucose by the three soluble PTS components, namely HPr, enzyme I, and factor III. A ptsG mutant defective in membrane-bound enzyme IIB′ of the high-affinity glucose transport system was shown to exhibit normal chemotaxis providing pleiotropic effects of the mutation were eliminated by its genotypic combination with other pts mutations or, phenotypically, by addition of cyclic AMP and substrate. A correlation was demonstrated between chemotaxis to glucose and activity of the low-affinity glucose transport complex, membrane-bound enzymes IIB:IIA, and an enzyme IIB:IIA mutant was shown to have a preponderant defect in chemotaxis to glucose and mannose. Of four systems capable of galactose transport, only the β-methylgalactoside transport system was implicated in chemotaxis to galactose. Some properties of a mutant possibly defective in processing of signals for chemotaxis to sugars is described.

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Selected References

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  • Adler J. Chemoreceptors in bacteria. Science. 1969 Dec 26;166(3913):1588–1597. [PubMed]
  • Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. [PubMed]
  • Adler J, Epstein W. Phosphotransferase-system enzymes as chemoreceptors for certain sugars in Escherichia coli chemotaxis. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2895–2899. [PMC free article] [PubMed]
  • Adler J, Hazelbauer GL, Dahl MM. Chemotaxis toward sugars in Escherichia coli. J Bacteriol. 1973 Sep;115(3):824–847. [PMC free article] [PubMed]
  • Aksamit RR, Howlett BJ, Koshland DE., Jr Soluble and membrane-bound aspartate-binding activities in Salmonella typhimurium. J Bacteriol. 1975 Sep;123(3):1000–1005. [PMC free article] [PubMed]
  • Bachmann BJ, Low KB, Taylor AL. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. [PMC free article] [PubMed]
  • Bochner BR, Savageau MA. Generalized indicator plate for genetic, metabolic, and taxonomic studies with microorganisms. Appl Environ Microbiol. 1977 Feb;33(2):434–444. [PMC free article] [PubMed]
  • Cordaro C. Genetics of the bacterial phosphoenolpyruvate: glycose phosphotransferase system. Annu Rev Genet. 1976;10:341–359. [PubMed]
  • Cordaro JC, Melton T, Stratis JP, Atagün M, Gladding C, Hartman PE, Roseman S. Fosfomycin resistance: selection method for internal and extended deletions of the phosphoenolpyruvate:sugar phosphotransferase genes of Salmonella typhimurium. J Bacteriol. 1976 Dec;128(3):785–793. [PMC free article] [PubMed]
  • Cordaro JC, Roseman S. Deletion mapping of the genes coding for HPr and enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system in Salmonella typhimurium. J Bacteriol. 1972 Oct;112(1):17–29. [PMC free article] [PubMed]
  • DAVIS BD, MINGIOLI ES. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. [PMC free article] [PubMed]
  • de Jong MH, van der Drift C, Vogels GD. Receptors for chemotaxis in Bacillus subtilis. J Bacteriol. 1975 Sep;123(3):824–827. [PMC free article] [PubMed]
  • Ferenci T, Kornberg HL. The role of phosphotransferase-mediated syntheses of fructose 1-phosphate and fructose 6-phosphate in the growth of Escherichia coli on fructose. Proc R Soc Lond B Biol Sci. 1974 Sep 17;187(1087):105–119. [PubMed]
  • Gutnick D, Calvo JM, Klopotowski T, Ames BN. Compounds which serve as the sole source of carbon or nitrogen for Salmonella typhimurium LT-2. J Bacteriol. 1969 Oct;100(1):215–219. [PMC free article] [PubMed]
  • Hazelbauer GL, Adler J. Role of the galactose binding protein in chemotaxis of Escherichia coli toward galactose. Nat New Biol. 1971 Mar 24;230(12):101–104. [PubMed]
  • Koshland DE., Jr A response regulator model in a simple sensory system. Science. 1977 Jun 3;196(4294):1055–1063. [PubMed]
  • Kundig W, Roseman S. Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the Escherichia coli phosphotransferase system. J Biol Chem. 1971 Mar 10;246(5):1407–1418. [PubMed]
  • Lengeler J. Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping. J Bacteriol. 1975 Oct;124(1):26–38. [PMC free article] [PubMed]
  • Lengeler J. Nature and properties of hexitol transport systems in Escherichia coli. J Bacteriol. 1975 Oct;124(1):39–47. [PMC free article] [PubMed]
  • Lo TC, Sanwal BD. Genetic analysis of mutants of Escherichia coli defective in dicarboxylate transport. Mol Gen Genet. 1975 Oct 22;140(4):303–307. [PubMed]
  • Melton T, Kundig W, Hartman PE, Meadow N. 3-Deoxy-3-fluoro-D-glucose-resistant Salmonella typhimurium mutants defective in the phosphoenolpyruvate:glycose phosphotransferase system. J Bacteriol. 1976 Dec;128(3):794–800. [PMC free article] [PubMed]
  • Mesibov R, Adler J. Chemotaxis toward amino acids in Escherichia coli. J Bacteriol. 1972 Oct;112(1):315–326. [PMC free article] [PubMed]
  • Ordal GW, Adler J. Isolation and complementation of mutants in galactose taxis and transport. J Bacteriol. 1974 Feb;117(2):509–516. [PMC free article] [PubMed]
  • Ordal GW, Adler J. Properties of mutants in galactose taxis and transport. J Bacteriol. 1974 Feb;117(2):517–526. [PMC free article] [PubMed]
  • Peterkofsky A, Gazdar C. Interaction of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system with adenylate cyclase of Escherichia coli. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2920–2924. [PMC free article] [PubMed]
  • Postma PW. Involvement of the phosphotransferase system in galactose transport in Salmonella typhimurium. FEBS Lett. 1976 Jan 1;61(1):49–53. [PubMed]
  • Postma PW. Galactose transport in Salmonella typhimurium. J Bacteriol. 1977 Feb;129(2):630–639. [PMC free article] [PubMed]
  • Simoni RD, Roseman S, Saier MH., Jr Sugar transport. Properties of mutant bacteria defective in proteins of the phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1976 Nov 10;251(21):6584–6597. [PubMed]
  • Strange PG, Koshland DE., Jr Receptor interactions in a signalling system: competition between ribose receptor and galactose receptor in the chemotaxis response. Proc Natl Acad Sci U S A. 1976 Mar;73(3):762–766. [PMC free article] [PubMed]
  • Van Der Drift C, De Jong MH. Chemotaxis toward amino acids in Bacillus subtilis. Arch Mikrobiol. 1974 Mar 4;96(2):83–92. [PubMed]
  • van der Drift C, Duiverman J, Bexkens H, Krijnen A. Chemotaxis of a motile Streptococcus toward sugars and amino acids. J Bacteriol. 1975 Dec;124(3):1142–1147. [PMC free article] [PubMed]
  • Yokota T, Gots JS. Requirement of adenosine 3', 5'-cyclic phosphate for flagella formation in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1970 Aug;103(2):513–516. [PMC free article] [PubMed]
  • Zukin RS, Strange PG, Heavey R, Koshland DE. Properties of the galactose binding protein of Salmonella typhimurium and Escherichia coli. Biochemistry. 1977 Feb 8;16(3):381–386. [PubMed]

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