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J Bacteriol. Jul 1982; 151(1): 269–273.
PMCID: PMC220237

Chromosomal mutation for citrate utilization by Escherichia coli K-12.

Abstract

A mutant strain of Escherichia coli K-12 that utilizes citrate as a sole source of carbon and energy was isolated. Citrate utilization arose as the consequence of two mutations in genes citA and citB, which are linked to the gal operon. The mutant strain expresses a semiconstitutive citrate transport system, and it utilizes both citrate and isocitrate as carbon and energy sources. It is capable of utilizing cis- and trans-aconitate, but only if it is preinduced by growth on citrate.

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Selected References

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  • Bachmann BJ, Low KB. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. [PMC free article] [PubMed]
  • Hall BG. Experimental evolution of a new enzymatic function. II. Evolution of multiple functions for ebg enzyme in E. coli. Genetics. 1978 Jul;89(3):453–465. [PMC free article] [PubMed]
  • Hall BG, Hartl DL. Regulation of newly evolved enzymes. I. Selection of a novel lactase regulated by lactose in Escherichia coli. Genetics. 1974 Mar;76(3):391–400. [PMC free article] [PubMed]
  • Gall BG, Hartl DL. Regulation of newly evolved enzymes. II. The ebg repressor. Genetics. 1975 Nov;81(3):427–435. [PMC free article] [PubMed]
  • Ishiguro N, Hirose K, Asagi M, Sato G. Incompatibility of citrate utilization plasmids isolated from Escherichia coli. J Gen Microbiol. 1981 Mar;123(1):193–196. [PubMed]
  • Ishiguro N, Oka C, Hanzawa Y, Sato G. Plasmids in Escherichia coli controlling citrate-utilizing ability. Appl Environ Microbiol. 1979 Nov;38(5):956–964. [PMC free article] [PubMed]
  • Ishiguro N, Oka C, Sato G. Isolation of citrate-positive variants of Escherichia coli from domestic pigeons, pigs, cattle, and horses. Appl Environ Microbiol. 1978 Aug;36(2):217–222. [PMC free article] [PubMed]
  • Koser SA. CORRELATION OF CITRATE UTILIZATION BY MEMBERS OF THE COLON-AEROGENES GROUP WITH OTHER DIFFERENTIAL CHARACTERISTICS AND WITH HABITAT. J Bacteriol. 1924 Jan;9(1):59–77. [PMC free article] [PubMed]
  • LARA FJS, STOKES JL. Oxidation of citrate by Escherichia coli. J Bacteriol. 1952 Mar;63(3):415–420. [PMC free article] [PubMed]
  • Lo TC, Rayman MK, Sanwal BD. Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells. J Biol Chem. 1972 Oct 10;247(19):6323–6331. [PubMed]
  • Lo TC, Sanwal BD. Genetic analysis of mutants of Escherichia coli defective in dicarboxylate transport. Mol Gen Genet. 1975 Oct 22;140(4):303–307. [PubMed]
  • Lütgens M, Gottschalk G. Why a co-substrate is required for anaerobic growth of Escherichia coli on citrate. J Gen Microbiol. 1980 Jul;119(1):63–70. [PubMed]
  • Winkler HH, Wilson TH. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed]
  • Wu TT. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. [PMC free article] [PubMed]

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