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J Cell Biol. May 14, 2001; 153(4): 835–850.
PMCID: PMC2192378

The Short Arm of the Laminin γ2 Chain Plays a Pivotal Role in the Incorporation of Laminin 5 into the Extracellular Matrix and in Cell Adhesion

Abstract

Laminin 5 is a basement membrane component that actively promotes adhesion and migration of epithelial cells. Laminin 5 undergoes extracellular proteolysis of the γ2 chain that removes the NH2-terminal short arm of the polypeptide and reduces the size of laminin 5 from 440 to 400 kD. The functional consequence of this event remains obscure, although lines of evidence indicate that cleavage of the γ2 chain potently stimulated scattering and migration of keratinocytes and cancer cells. To define the biological role of the γ2 chain short arm, we expressed mutated γ2 cDNAs into immortalized γ2-null keratinocytes. By immunofluorescence and immunohistochemical studies, cell detachment, and adhesion assays, we found that the γ2 short arm drives deposition of laminin 5 into the extracellular matrix (ECM) and sustains cell adhesion. Our results demonstrate that the unprocessed 440-kD form of laminin 5 is a biologically active adhesion ligand, and that the γ2 globular domain IV is involved in intermolecular interactions that mediate integration of laminin 5 in the ECM and cell attachment.

Keywords: keratinocyte, epithelial adhesion, cell migration, basement membrane, epidermolysis bullosa

Introduction

The epithelial basement membranes consist of a complex network of extracellular matrix (ECM) molecules that mediate tissue integrity and homeostasis, and control morphogenesis as well as tissue repair and tumorigenesis. Laminins are multifunctional glycoproteins of the ECM that contribute to the architecture of the basement membranes and play a crucial role in cell adhesion, growth, migration, and differentiation (Colognato and Yurchenco 2000). These cross-shaped molecules consist of three subunits, classified as α, β, and γ chains on the basis of their primary structure deduced from sequence data. Laminin isoforms are expressed at various stages of development and in specific tissue locations in different species. In the human epidermis, basal keratinocytes express laminin 5 (α3β3γ2), laminin 6 (α3β1γ1), and laminin 10 (α5β1γ1) (Champliaud et al. 1996).

Laminin 5 is found in the basement membrane of stratified and transitional epithelia, prevalently associated with the extracellular anchoring filaments of the lamina lucida that connect the hemidesmosomes to the anchoring fibrils of the underlying stroma (Verrando et al. 1987; Rousselle et al. 1991; Masunaga et al. 1996). Laminin 5 is a specific substrate for adhesion in proliferating and migrating keratinocytes (Rousselle et al. 1991; Rousselle and Aumailley 1994). The biological relevance of laminin 5 in dermal–epidermal adhesion has been emphasized by the identification of genetic mutations affecting laminin 5 in patients with junctional epidermolysis bullosa (JEB), an inherited genodermatosis characterized by fragility of the skin and blistering in response to minor trauma (Aberdam et al. 1994a; Pulkkinen et al. 1994). The adhesive role of laminin 5 was also confirmed by the detection of circulating autoantibodies against epitopes of laminin 5 in patients with acquired blistering skin disorders which are characterized by dermal–epidermal cleavage (Yancey et al. 1995; Lazarova et al. 1996). Laminin 5 has been reported to influence morphogenetic events (Baker et al. 1996; Stahl et al. 1997), to affect cell motility (Zhang and Kramer 1996; O'Toole et al. 1997), to enhance invasiveness in human cancers (Kikkawa et al. 1994; Pyke et al. 1994, Pyke et al. 1995; Giannelli et al. 1997; Koshikawa et al. 1999), and to control cell growth (Ryan et al. 1999).

Current models propose that laminin 5 mediates epithelial cell adhesion via integrin α3β1 in focal adhesions and integrin α6β4 in hemidesmosomes (Carter et al. 1991; Sonnenberg et al. 1993; Mainiero et al. 1995; Baker et al. 1996; Goldfinger et al. 1998). It has been shown that the domain(s) interacting with the cell surface receptors reside within the COOH-terminal G domains of the protein (Baker et al. 1996; Mizushima et al. 1997; Hirosaki et al. 2000). Conversely, the precise contribution of the NH2 terminus of the three chains of laminin 5 to cell adhesion is not yet well understood. In laminins, in general, the NH2-terminal domains are thought to mediate polymerization into a network that stabilizes the basement membrane (Colognato and Yurchenco 2000). Compared with the other laminin isoforms, the short arms of the α3, β3, and γ2 chains are substantially truncated and do not conserve the domains known to direct integration of laminins into the basement membrane architecture (Cheng et al. 1997; Colognato and Yurchenco 2000). However, it has been proposed that the NH2 terminus of laminin 5 can interact with laminin 6 and 7, and also with the NC-1 domain of type VII collagen, the major component of anchoring fibrils (Marinkovich et al. 1992; Champliaud et al. 1996; Chen et al. 1997; Rousselle et al. 1997). On these bases, it has been suggested that monomeric laminin 5 molecules within the anchoring filaments bridge integrin α6β4 to type VII collagen and provide the significant force that promotes cohesion of the dermis and epidermis, whereas laminin 5 complexed to laminins 6 and 7 interacts with integrin α3β1 and contributes to assembly and stability of the basement membrane (DiPersio et al. 1997; Rousselle et al. 1997). Binding to type VII collagen NC-1 domain appears to occur within the β3 or γ2 short arms (Rousselle et al. 1997; Chen et al. 1999). Formation of the disulfide-bonded complex between laminin 5 and laminins 6 and 7 could involve the uncoupled cysteinyl residues harbored by the globular domain VI of the chain β3 and the domain IIIa of the α3 chain (Gerecke et al. 1994; Amano et al. 2000). It has also been proposed that binding of the basement membrane component fibulin 2 through the globular domain IV of the γ2 chain associates mouse laminin 5 to the matrix proteins (Utani et al. 1997). In laminin 5–null keratinocytes, the adhesion function of the protein is not complemented by other laminins (Vidal et al. 1995; Miquel et al. 1996).

In the ECM, laminin 5 is found in two most abundant forms of 440 and 400 kD. The 440-kD heterotrimer is generated by proteolytic cleavage of the α3 chain present in the 460-kD cell-associated form of laminin 5 (Marinkovich et al. 1992). Extracellular processing of the α3 that excises the COOH terminus at a cleavage site within the subdomain G4, and proteolytic cleavage within the EGF-like repeat 2 of domain IIIa, have also been reported (Champliaud et al. 1996; Goldfinger et al. 1998; Amano et al. 2000). The proteolytic processing of the α3 chain G domain is thought to modulate the interactions of laminin 5 with the integrin cell receptors and to govern cell anchorage and motility (Goldfinger et al. 1998).

Extracellular processing also removes the globular domain IV and the EGF-like rich domain V of the laminin γ2 short arm. Excision of the 434 NH2-terminal amino acids shortens the γ2 chain from 155 to 105 kD, and reduces the size of laminin 5 to 400 kD (α3, 165 kD; β3, 140 kD; γ2, 105 kD). Therefore, this processed 400-kD form of the laminin 5 lacks most of the γ2 chain short arm, but leaves the β3 short arm intact. The functional role of the γ2 cleavage is unknown and the biological functions specific to each extracellular form of laminin 5 remain obscure.

Extracellular 440-kD laminin 5 has so far been considered a precursor form of the fully processed laminin 5. To define the functions of this molecule, and to identify the possible biological role of the γ2 short arm excised by the proteolytic processing, we have transferred mutated laminin γ2 cDNAs into immortalized γ2-null keratinocytes. In this study, we report that the laminin γ2 short arm is required for deposition of laminin 5 into the ECM, and we provide evidence that the unprocessed 440-kD form of laminin 5 sustains cell adhesion and inhibits cell migration.

Materials and Methods

cDNA Constructs

Mutated laminin γ2 constructs were obtained by site-directed mutagenesis of a full length γ2 cDNA cloned in the expression vector pγWT (Gagnoux-Palacios et al. 1996) in which the antigenic peptide sequence MASMTGGQQMG (T7-Tag) of T7 bacteriophage was inserted 3′ to the coding sequence of the γ2 cDNA (see Fig. 1 B). The presence of the T7-Tag does not affect the laminin 5 function (data not show). To construct mutant pγNC, two γ2 cDNA fragments of 1,308 and 476 bp (comprising nt 1–1293 and 1305–1767 of the laminin γ2 cDNA, respectively; EMBL/GenBank/DDBJ accession no. X73920) were PCR amplified using plasmid pγWT as a template. The 1,308-bp cDNA fragment was amplified using primers A, left and B, right (Table ). The reaction mixture (25 μl) contained 10 ng of the template, 400 nM of each primer, 20 mM Tris-HCl, pH 8.8, 2 mM MgSO4, 10 mM KCl, 10 mM (NH4)2SO4, 0.1% Triton X-100, 100 μg/ml nuclease-free BSA, 200 mM dNTP, and 2.5 U of Pfu DNA polymerase (Stratagene). For amplification of the 476-bp cDNA fragment, primers were: C, left, and D, right. The primary PCR products were mixed and used as templates for a secondary PCR program using primers A and D in a reaction mixture of 25 μl. The PCR products were electrophoresed on a 1% agarose gel, eluted, mixed together, cleaved with the restriction endonucleases BsmBI and EcoRI, and cloned into a BsmBI-EcoRI–digested pγWT vector. To generate pγC, a γ2 cDNA with an internal deletion of 1,208 bp (nt 1302–94) was prepared by PCR amplification of two γ2 cDNA fragments: a 107-bp γ2 cDNA fragment (nt 1–93) using primer A, left, and E, right, and a 478-bp γ2 cDNA fragment (nt 1,303–1,766) using primers F, left, and D, right. The amplification products were subjected to a secondary PCR amplification using primers A and D. The resulting cDNA products were cloned into a BsmBI-EcoRI–digested pγWT plasmid as described above. To obtain plasmid pγM, a 1,081-bp (nt 1–1065) and a 496-bp (nt 1286–1766) cDNA fragments were amplified using the primer pair A, left, and G, right and pair H, left and D, right, respectively. After a secondary PCR amplification using primers A and D, the resulting amplimers were cloned into plasmid pγWT as described above. To generate the mutant pγIII, which bears an internal deletion of 101 bp, two γ2 cDNA fragments of 1,159 and 536 bp were PCR amplified using the primers pair A, left and I, right, respectively, and primer pair J, left and D, right. After a secondary PCR amplification using primers A and D, the resulting amplimers were cloned into plasmid pγWT as described above. To construct pγV, which contains an internal deletion of 242 bp, pγWT was double digested with Bsu36I and BspEI (equals AccIII), blunted, and then religated. Plasmid pγF1 was generated using the QuikChange™ site-directed mutagenesis kit (Stratagene) and primers K, left and L, right to substitute the amino acid residues SVHKI (residues 203–207) with five alanines. Plasmid pγF2 was generated from pγF1, using the QuikChange™ kit and the oligonucleotide pair M, left and N, right to subsitute the peptidic sequence SAEYSVHKI (residues 199–207) with nine alanines. Plasmid pγGP was generated using the QuikChange™ kit and primers O, left and P, right to delete the amino acid residues YS (residues 432–433) and substitute the amino acid residues GD (residues 434–435) with the amino acid residues GP. The conformational changes of the γ2 chain introduced by the GP substitution were assessed using the programs Biopolymer (Molecular Simulations, Inc.) and Antheprot (http://www.ibcp.fr).

Table 1
Nucleotide Sequence of the Primers Used in Site-directed Mutagenesis of the Laminin γ2 cDNA
Figure 1Figure 1
Schematic representation of laminin 5 and the γ2 chain mutants used in this study. (A) The EGF-like repeats (□) and the globular domains (○) of the α3, β3, and γ2 chains of laminin 5 are depicted. Full gray ...

To generate plasmid pγ50, the 1,302-bp γ2 cDNA fragment (nt 1–1302) coding for the NH2-terminal domains of the laminin γ2 chain was subcloned into the BamH1-EcoRV sites of the expression vector pcDNA3, upstream the T7-Tag. To construct plasmid pβ60 the 1,574-bp cDNA sequence encoding the NH2-terminal domains of the laminin β3 chain (nt 1–1574; EMBL/GenBank/DDBJ accession no. L25541) was subcloned into pcDNA3 upstream the hemagglutinin (HA) Tag sequence (YPYDVPDYA).

The plasmids were amplified in Escherichia coli XL1blue (Stratagene), purified using a plasmid purification kit (QIAGEN), and analyzed by nucleotide sequencing using an ABI Prism 310 genetic analyzer.

Antibodies

mAbs used in the study were: K140, specific to laminin β3 (Marinkovich et al. 1992); GB3, directed against native laminin 5 (Verrando et al. 1987); T7-Tag, specific to the peptide sequence (MASMTGGQQMG) of T7 bacteriophage (Novagen); and anti-HA (clone 12CA5), specific to the peptide sequence (YPYDVPDYA) of the influenza HA protein (Santa Cruz Biotechnology, Inc.).

Expression of laminin γ2 chain was examined using polyclonal antibody (pAb) SE144 (Vailly et al. 1994). pAb SE1097 was raised against the fusion protein corresponding to the laminin γ2 short arm. To prepare the fusion protein, the γ2 cDNA fragment was excised from plasmid pγ50 and subcloned into the EcoRI site of the procaryotic expression vector pGEX-5X-3 from Amersham Pharmacia Biotech. The purified GST fusion protein was used for rabbit immunization. pAb SE1097 was then purified by immunoaffinity chromatography against the corresponding fusion protein immobilized on nitrocellulose filters after SDS-PAGE. The second antibodies coupled to tetramethylrhodamine isothiocyanate or to fluoresceine isothiocyanate were obtained from Dako. Actin cytoskeleton was labeled using phalloidin Texas red (Molecular Probes, Inc.).

Cells and Organotypic Cell Cultures

The human keratinocytes cell lines HKSV and LSV5 were grown in a 3:1 mixture of DME and Ham's F-12 medium (Life Technologies and GIBCO BRL) containing FCS (10%), hydrocortisone (0.4 μg/ml), cholera toxin (0.1 nM), and EGF (10 ng/ml) (Miquel et al. 1996). Swiss mouse 3T3-J2 cells and the African monkey kidney cell line COS-7 were cultured in DME containing 10% calf serum. Secondary cultures of human keratinocytes HNKs were grown on irradiated feeder layers of mouse 3T3-J2 cells in DME/Ham's F12 medium as described above and supplemented with insulin (5 μg/ml), adenine (0.18 mM), triiodothyronine (2 nM), glutamine (4 mM), and penicillin-streptomycin (50 IU/ml) (Rheinwald and Green 1975).

To construct artificial epithelia, cultured keratinocytes (1.6 × 107 cells) were seeded in stainless steel rings (0.6 cm2), laid on acetate cellulose filters (Millipore), and maintained immersed in growth medium changed every other day. 4 d later, the medium in the rings was removed and the keratinocyte culture was brought at the air–liquid interface for 4 d to induce differentiation (Rosdy et al. 1993). The artificial epithelia were then included in OCT compound Tissue-Tek (Miles Laboratories) and immediately frozen in liquid nitrogen. Vertical 4-μm sections were used for indirect immunofluorescence studies.

DNA Transfections

For transient expression, subconfluent keratinocyte cultures were transfected using the polycationic lipid Dosper (1,3-Di-Oleoyloxy-2,6-[carboxy-spermyl]-propyl-amide) from Roche. In brief, before transfection the cell cultures were incubated at 37°C with medium deprived of serum and growth factors. DNA and Dosper were separately diluted in Hepes-buffered saline (20 mM Hepes, 150 mM NaCl, pH 7.4), then mixed at a final concentration of 0.2 and 0.1 μg/ml, respectively, and added dropwise to the cell layers. After 6 h at 37°C, cells were fed with medium containing serum and growth factors two times concentrated. Transfection efficiency and expression of the transfected genes was monitored 48 h later using indirect immunofluorescence assays.

To establish cell lines LγNC, LγC, LγGP, LγF1, and LγF2 using plasmids pγNC, pγC, pγGP, pγF1, and pγF2, respectively, calcium-phosphate transfection of LSV5 cells was performed using the MBS™ mammalian transfection kit (Stratagene). 1.5 × 105 cells were seeded in 100-mm petri dishes and incubated for 24 h at 37°C. 20 μg of plasmid DNA precipitated for 15 min at room temperature in BES buffer (50 mM N,N-Bis2hydroxyethyl-2-aminoethan sulfonic acid, 250 mM NaCl, 1.5 mM Na2HPO4, pH 6.95) containing 125 mM CaCl2 was added dropwise to the cell layers. The cell cultures were incubated for 3 h in DME supplemented with 5% modified bovine serum provided with the transfection kit, then rinsed and fed with DME/Ham's F12 medium. Selection for neoresistance to geneticin (400 μg/ml; Sigma-Aldrich) was started 48 h later.

Immunofluorescence Microscopy

Immunofluorescence analysis of subconfluent cell layers grown on glass coverslips and fixed in PBS, pH 7.4, containing 1 mM CaCl2, 1 mM MgCl2, and 3% formaldehyde were processed as reported (Gagnoux-Palacios et al. 1996). Semithin sections of frozen samples of organotypic cultures were fixed and analyzed as described (Rosdy et al. 1993). Cells monolayers and sections were analyzed using a ZEISS Axiophot microscope.

Immunoprecipitation and Immunoblotting Studies

Immunoblotting and immunoprecipitation analysis of the cell extracts and samples of culture medium were as already described (Gagnoux-Palacios et al. 1996). For Western analysis, the ECM was detached by scraping the plastic tissue culture dish in the presence of 2% SDS, 10% glycerol, 100 mM β-mercaptoethanol, and 50 mM Tris-HCl, pH 6.8. The samples were boiled, electrophoresed in a 7.5% SDS-polyacrylamide gel, and blotted onto nylon filters. The antibody–antigen complex was revealed using the ECL Western blotting kit (Amersham Pharmacia Biotech).

Cell Detachment Assay

Cells (2 × 104/cm2) were seeded in tissue culture flasks and incubated for either 12 or 48 h at 37°C to reach 50 and 80% confluence, respectively. The monolayers were then treated with a solution of trypsin/EDTA (Versene and BioWhittaker) diluted 1:70 in PBS. The number of cells detached at increasing time of incubation was determined by collecting the supernatants and direct cell counting. Each experiment was repeated six times. A representative experiment is shown.

Quantification of Laminin 5 Deposited in the ECM by Cultured Keratinocytes

Laminin 5 deposition was quantified both on plastic cell culture substrate and on plastic coated with ECM components. To coat the plastic substrate, multiwell plates (96 wells; TPP) were incubated with solutions of collagen type I or IV, vitronectin, fibronectin, laminin 1, or ECM (all from Sigma-Aldrich) dissolved in PBS at a concentration of 10 μg/ml. 104 cells per well were seeded and incubated for either 12 or 48 h at 37°C in humidified atmosphere in the presence of 5% CO2. The cells were then washed twice in PBS and detached as devised by Delwel et al. 1993. In brief, the cells were incubated overnight at 4°C in PBS containing 20 mM EDTA, leupeptin (10 μg/ml), aprotinin (10 μg/ml), phenylmethanesulfonyl fluoride (1 mM), and soybean trypsin inhibitor (10 μg/ml) and then dislodged by pipetting. The matrices were washed with PBS, incubated for 10 min with PBS-0.2% Triton X-100 to remove cell debris, and saturated for 1 h at room temperature in PBS-1% BSA. Each well was incubated for 2 h at room temperature in 50 μl PBS-1% BSA containing mAb GB3 (10 μg/ml). The plates were washed seven times with PBS and incubated for 1 h at room temperature with 50 μl of PBS-1% BSA containing 1 μg/ml of anti–mouse HRP mAb (Dako) per well. After extensive washing, 50 μl of a solution of 0-phenylenediaminedihydrochloride (Sigma-Aldrich) was added to each well for 10 min in the dark as devised by the supplier. Color yields were determined at 490 nm in an Elisa reader (Dynatech). Values were expressed as percentage of the values obtained with mutant LγNC cells.

Adhesion Assay

Multiwell plates containing the ECM secreted by the different mutant cell lines were prepared as described. Wells were saturated for 1 h at room temperature with a solution of 0.5% heat-denatured BSA. Wild-type HNKs (3 × 104 cells per well) were plated and fed with serum-deprived medium containing 0.25% heat-denatured BSA. After 1 h at 37°C, the medium and the cells in suspension were removed and the wells were washed with PBS. Adherent cells were fixed in 3.7% formaldehyde, stained with 0.5% crystal violet dissolved in 20% methanol, and washed three times in PBS. The dye was eluted with 50% ethanol/0.1 M sodium citrate, pH 4.2. Absorbance at 540 nm was determined using a microplated reader. Values were expressed as a percentage of the values obtained with mutant LγNC cells.

Results

The functional role of the short arm domains of the laminin γ2 chain was investigated by transfecting a series of mutant γ2 cDNAs into keratinocyte cell line LSV5. Cell line LSV5 is derived from the keratinocytes of an H-JEB patient with a homozygous nonsense mutation (R95X) in the gene (LAMC2) coding for the laminin γ2 chain (Miquel et al. 1996). LSV5 cells do not synthesize the laminin γ2 chain, but express the full repertoire of the laminin chains found in HNKs, including the laminin α3 and β3 chains (Miquel et al. 1996). Transfection of a wild-type γ2 cDNA restores production of functional α3β3γ2 laminin 5 molecules (Gagnoux-Palacios et al. 1996). The complementary DNAs coding for mutant laminin γ2 chains were generated by directed mutagenesis of the expression vector pγWT which encodes the full length γ2 polypeptide (Gagnoux-Palacios et al. 1996). As depicted in Fig. 1 B, the mutant cDNA pγNC expresses a recombinant γ2 polypeptide with an internal deletion encompassing the four amino acids (YSGD) within domain III that constitute the proteolytic cleavage site of the γ2 chain (Vailly et al. 1994; Amano et al. 2000). In mutant pγGP, the GlyPro residues substitute the YSGD cleavage site and introduce a structural modification from “sheet” to “coil” configuration of the γ2 chain domain III. Mutants pγIII and pγV carry a deletion affecting the egf-like repeat 1 of domain III and repeats 2 and 3 of domain V, respectively. To investigate the functional role of the laminin γ2 chain short arm, we generated a cDNA clone encoding a polypeptide lacking the NH2-terminal domains IV and V that are excised in the extracellular processing of laminin 5 (plasmid pγC). Further, a polypeptide with an internal deletion encompassing the EGF-like repeat 1 of domain III and the COOH-terminal portion of domain IV (plasmid pγM) was also constructed. Plasmid pγM corresponds to a mutated γ2 chain missing 73 amino acids of the polypeptide sequence detected in a patient suffering from JEB (Pulkkinen et al. 1994). The deletion was thought to interfere with the extracellular processing of the polypeptide (Amano et al. 2000).

The different mutant cDNAs were transiently transfected into actively growing LSV5 keratinocytes. Immunocytochemical analysis of the transfected cultures using pAb SE144 (not shown) and mAb GB3 indicated that all the mutant γ2 polypeptides were actively synthesized and incorporated into laminin 5 heterotrimers (Fig. 2). The ECM deposited on the culture support by cells LγWT, LγNC, LγΔIII, LγΔV, and LγGP transfected with plasmids pγWT, pγNC, pγΔIII, pγΔV, and pγGP was immunoreactive, whereas the ECM deposited by the cell cultures LγC and LγM transfected with plasmids pγC and pγM was not labeled. These results indicate that deletions within the globular domain IV of the γ2 chain prevent secretion and/or deposition of laminin 5 into the ECM, but do not limit laminin 5 synthesis.

Figure 2Figure 2
Immunofluorescence analysis of laminin 5 synthesis in LSV5 keratinocytes transiently transfected with plasmids encoding the mutant γ2 chains. LSV5 untransfected (a and b) and transfected with pγWT (c and d), pγNC (e and f), pγC ...

To confirm the role of the globular domain of the γ2 polypeptide in deposition of laminin 5 to the cell culture substratum, plasmids pγNC, pγGP, and pγC were stably transfected into LSV5 keratinocytes. Cell lines LγNC and LγGP were generated that are expected to secrete a mutated 440-kD form of laminin 5 with a full length γ2 chain (155 kD), whereas cell line LγC is expected to produce a 400-kD molecule corresponding to laminin 5 with a processed γ2 chain (105 kD). Consistent with the results obtained with transiently transfected LSV5 cells, immunocytochemical analysis of LγNC, LγGP, and LγC cells using pAb SE144 and mAb GB3 detected a strong cytoplasmic labeling in all cell lines. These experiments also confirmed that only the ECM laid down by LγNC and LγGP keratinocytes contains immunoreactive laminin 5 epitopes (not shown). These results indicate that presence of the γ2 short arm is required for deposition of laminin 5 to the culture substratum, but do not rule out the possibility that laminin 5 harboring a γC recombinant chain is secreted into the media without being incorporated into ECM. Expression and secretion of the recombinant γNC, γGP, and γC polypeptides were examined by Western blot analysis of spent medium of LγNC, LγGP, and LγC cells. A unique 105-kD migration band was detected in the LγC medium using pAb SE144, whereas a specific 155-kD band was observed with LγNC and LγGP cells (Fig. 3 A). The intensity of these bands was comparable and was estimated to be threefold weaker than the intensity of the corresponding bands detected in the medium conditioned by wild-type keratinocytes. Thus, although the γ2 short arm is required for incorporation into the ECM, it is not needed for secretion of γ2 chain.

Figure 3
Deposition to the culture substratum of mutant laminin 5 molecules is dependent on the nature of the γ2 chain. (A) Western analysis of spent medium of cultured keratinocytes. 50 μg of proteins from spent medium collected from cultures ...

Incorporation of the recombinant γ2 polypeptides into extracellular laminin 5 molecules was further verified by immunoprecipitation of LγC and LγNC cell culture medium using the mAb K140 and the anti–T7-Tag mAb. As shown in Fig. 3 B, comparable amounts of 400- and 440-kD laminin 5 molecules were immunoprecipitated from the LγNC and LγC cell medium, respectively, which attests to the assembly of the 105- and 155-kD recombinant γ2 polypeptides into laminin 5. These data demonstrate that the absence of the γ2 NH2-terminal domains does not hinder the intracellular processing of laminin 5 and its secretion into the culture medium. They also confirm that the tetrapeptide YSGD is the unique physiological cleavage site of the extracellular processing of the γ2 chain.

Artificial epithelia constructed either with HNKs or LSV5 cells expressing a recombinant wild-type γ2 chain have been shown previously to lay down laminin 5 at the interface between the basal cells and the cell culture support (Rosdy et al. 1993; Gagnoux-Palacios et al. 1996; Miquel et al. 1996). Because truncation of the γ2 short arm appeared to prevent deposition of laminin 5 on monolayer submersed cultures, LγC and LγNC keratinocytes were grown to confluence on cellulose acetate filters and exposed to air to obtain stratification into multilayered epithelia. Immunofluorescence analysis of the artificial epithelia using mAb GB3 detected a strong reactivity in the case of LγNC and HKSV cells (Fig. 3 C, b and d), and no reactivity with LSV5 and LγC keratinocytes (Fig. 3 C, a and c). Therefore, these observations confirm that the 400-kD form of laminin 5 produced by LγC keratinocytes is not incorporated into the ECM, and underscore the importance of the γ2 short arm plays in the deposition of laminin 5 at the epithelial–ECM interface.

Because our results suggested that the laminin γ2 short arm is essential for the incorporation of laminin 5 into the ECM secreted by LSV keratinocytes, we verified whether human skin and the matrix secreted by wild-type keratinocytes contain the NH2-terminal γ2 polypeptide generated by the extracellular processing of the 440-kD laminin 5. Specific antiserum pAb SE1097 was generated against recombinant fragment γ50, which is cleaved from the short arm of the γ2 chain (Fig. 1 B). The antibody stained the human epidermal basement membrane in a strong linear fashion (Fig. 4A and Fig. B), comparable to the labeling observed with antibody SE144 (not shown). Samples of the ECM produced by cultures of wild-type keratinocytes were then collected from culture dishes and analyzed by immunoblotting using pAb SE144 and pAb SE1097. As shown in Fig. 4 C, pAb SE144 recognized the 155- and 105-kD migration bands corresponding to the unprocessed and processed γ2 polypeptide, respectively. pAb SE1097 identified the unprocessed 155-kD γ2 chain and an additional fast-migrating band with the expected mass (50 kD) of the NH2-terminal domains, which are extracellularly excised from the γ2 chain (Fig. 4 D). The finding that only unprocessed γ2 chain is found intracellularly and that the processed γ2 chain and its cleavage fragment are found in the ECM indicate that the 440-kD laminin 5 molecules are proteolytically cleaved into the 400-kD form after incorporation into the ECM. This strengthens the idea that the γ2 short arm plays a role in the integration of laminin 5 in the matrix.

Figure 4
Laminin γ2 short arm drives deposition of laminin 5 to the ECM. Immunofluorescence analysis of control (A) and split skin (B) using pAb SE1097. The strong linear staining indicates that the 440-kD form of laminin 5 and/or the NH2-terminal cleaved ...

To verify this hypothesis, wild-type keratinocytes were transfected with the construct pγ50 encoding the NH2-terminal domains IV and V of γ2 carrying a T7-Tag peptide (Fig. 1). Control cultures were transfected with an expression vector (pβ60) that encodes the NH2-terminal domains III, V, and VI of the laminin β3 chain tagged with the HA epitope (Fig. 1). As shown by immunofluorescence microscopy, mAb T7-Tag reacted with the cytoplasm of the keratinocytes transfected with plasmid pγ50 and labeled the ECM deposited by these cells (Fig. 4 E, a and b). Conversely, the anti-HA mAb stained the cytoplasm of the keratinocytes expressing the recombinant cDNA pβ60, but not the ECM they deposited (Fig. 4 E, c and d). Similar results were obtained by transfection experiments performed using LSV5 cells (not shown). These observations suggest that the short arm of the laminin γ2 chain carries domains essential to the incorporation of laminin 5 into the matrix.

It has been suggested that the globular domain IV of the mouse laminin γ2 chain binds to the ECM protein fibulin 2 (Utani et al. 1997). However, residue Phe-202 of the γ2 amino acid sequence, which is essential to the interaction, is not conserved in humans. In addition, we were unable to demonstrate interactions between human laminin 5 and fibulin 2 in our experimental conditions (data not shown). Intriguingly, the amino acid sequence of the NH2-terminal region of the γ2 domain IV is highly conserved in mammals (Table ), which may reflect a relevant physiological role of this portion of the polypeptide. Indeed, it was suggested that disruption of the fibulin 2 binding site could hamper the proteolytic processing of γ2 (Utani et al. 1997). Therefore, we constructed plasmids pγF1 and pγF2 that encode γ2 polypeptides in which the amino acid sequence SADFSVHKI (residues 199–207) homologous to the active site of the mouse fibulin 2 binding site was partially (pγF1) and totally (pγF2) substituted by alanine residues (Fig. 1). LSV5 keratinocytes transfected with constructs pγF1 and pγF2 were examined by immunofluorescence analysis using mAb GB3. Expression of the mutant laminin 5 molecules resulted in a strong staining of the cytoplasm, and also of the ECM deposited onto the tissue culture support (Fig. 5 A, a and c). Western blot analysis of medium collected from cultures of LγF2 keratinocytes using pAb SE144 detected hybridization bands corresponding to the uncleaved (155 kD) and the proteolytically cleaved (105 kD) pγF2 chain (Fig. 5 B). According to these observations, immunofluorescence examination of frozen sections of artificial epithelia constructed using LγF1 and LγF2 keratinocytes detected a strong reactivity of the basement membrane zone to mAb GB3 (Fig. 5 C, a and b). These results attest to the incorporation of the mutant laminin 5 molecules into the ECM deposited on the cell culture substrate and show that disruption of the region homologous to the putative fibulin 2 binding site of the mouse γ2 short arm does not interfere with the processing and deposition of laminin 5 to the matrix.

Table 2
Alignment of the Laminin γ2 cDNA Sequences Homologous to the Active Site of Fibulin 2 Binding in the Mouse
Figure 5
Secretion and deposition on the ECM of mutants LγF1 and LγF2 laminin 5 molecules. (A) Immunofluorescence staining of LSV5 keratinocytes transiently transfected with plasmids pγF1 (a and b) and pγF2 (c and d). Double immunofluorescence ...

Reexpression of wild-type laminin 5 restores adhesion of LSV5 cells and H-JEB keratinocytes (Gagnoux-Palacios et al. 1996; Vailly et al. 1998). Because the 400-kD molecules of laminin 5 produced by LγC keratinocytes are not deposited into the ECM, LγC cells are expected to retain the poor adhesion capacity of the parental cell line LSV5. Indeed, epidermal sheets of stratified epithelium generated by confluent cultures of LγC, LγGP, and LγF1 keratinocytes spontaneously detached from the culture vessel. In contrast, when LγNC keratinocytes became confluent and stratified, the epidermal sheet firmly adhered to the plastic dish and detachment required enzymatic treatment (not shown). Therefore, attachment of LγC keratinocytes was quantified in detachment kinetic assays in the presence of trypsin/EDTA (Vailly et al. 1998). The attachment capacity of LγNC keratinocytes was also compared with that of wild-type keratinocytes. Cell suspensions were seeded on petri dishes to obtain exponentially growing cultures. 12 h after plating, the percentage of the adhering LγNC and LγC cells was similar to that of parental LSV5 cells (Fig. 6 A). Although the number of LγNC and LγWT cells resistant to trypsinization increased 48 h after seeding (50% of LγNC cells were dislodged after 14 min), that of LγC and LSV5 cells remained low (50% of dislodged cells after 8 min; Fig. 6 B). Therefore, the progressive enhancement of adhesion of LγΝC and LγWT keratinocytes appeared to correlate with accumulation of laminin 5 molecules harboring the γ2 short arm in the matrix. Detachment of LγGP and LγF1 keratinocytes was also assessed in similar experimental conditions. As demonstrated in Fig. 6 B, in all these cells other than LγNC synthesis of mutated laminin 5 molecules had no appreciable effect on the strength of cell adhesion.

Figure 6
Detachment assay of keratinocytes expressing mutant laminin 5 and quantification of laminin 5 layered down to the culture substrate. For the detachment assay, cells (2 × 104/cm2) were seeded on a plastic cell culture vessel, left to adhere for ...

To confirm that resistance to trypsinization of LγNC cells correlates with laminin 5 incorporation in the ECM, the amount of laminin 5 layered down by the different γ2 mutant keratinocytes was determined by ELISA assay using mAb GB3. As shown in Fig. 6 C, laminin 5 produced by LγNC cells was efficiently deposited on the plastic culture substrate and accumulated with increasing time, whereas mutant γC laminin 5 was inefficiently layered down. LγWT, LγGP, or LγF1 cells layered down comparable amounts of laminin 5. In addition, by seeding the mutant keratinocytes in a plastic vessel coated with different components of the ECM, we assessed the deposition rate of the wild-type and mutant laminin 5 molecules to be independent from the nature of the cell culture substrate on which the keratinocytes are grown (Fig. 7). Plating on a feeder of irradiated mouse 3T3-J2 cells did not modify the deposition pattern of laminin 5 (not shown).

Figure 7
Deposition of mutant laminin 5 is not affected by the cell culture substrate. LSV5 keratinocytes expressing mutant laminin 5 (104 cells per well) were seeded in 96-multiwell plates coated with 10 μg/ml of collagen type I (coll I), collagen type ...

Because the trypsinization assay provides information on the effect of laminin 5 on the strength of cell attachment, the functional role of the laminin γ2 short arm in cell adhesion was further assessed by seeding wild-type primary human keratinocytes on the ECM deposited by the different LSV5 γ2 mutants. The mutant cells were seeded in a range of concentrations leading to deposition of equivalent amounts of laminin 5 48 h after plating. The ECM was prepared after detachment of the cells by EDTA treatment, and concentration of laminin 5 in the ECM was checked by ELISA assays using mAb GB3. Primary wild-type keratinocytes were then seeded and allowed to adhere for 60 min at 37°C. Results show that adhesion of keratinocytes on the matrix secreted by LγNC and LγWT cells was comparable, whereas adhesion on the matrix produced by LγC cells was strongly reduced and equivalent to the values obtained with matrix produced by the γ2-null LSV5 cells (Fig. 8). Adhesion on the matrix produced by the LγF1 and LγGP mutant cells was also reduced to a lesser extent. This result suggests that deposition of these laminin 5 molecules allows interaction of their COOH-terminal G domain with the adhesive integrin receptors at the cell surface, although participation of other cell receptors in adhesion cannot be excluded. However, the cell detachment assay shows that the adhesion strength of mutant LγF1 and LγGP cells is affected, which may reflect a lower binding capacity between laminin 5 secreted by these cells and the culture substrate (Fig. 7 B).

Figure 8
The unprocessed form of laminin 5 promotes cell adhesion. Wild-type keratinocytes (NHK) were seeded on ECM secreted by LSV5 cell expressing laminin 5 harboring the mutant γ2 chains. Cell were left to adhere for 1 h at 37°C. Cell adhesion ...

Because keratinocyte adhesion was measured on a matrix produced by the different LSV mutant cells, we could not determine whether the adhesive effect we observed was directly or indirectly sustained by laminin 5. Nevertheless, our observations demonstrate that the laminin 5 molecules harboring an unprocessed γ2 chain are biologically active and indicate that structural changes in the NH2-terminal region of the globular domain IV affect the biological function of laminin 5.

Discussion

Transfection of a wild-type laminin γ2 cDNA in LSV5 keratinocytes rescues expression of functional laminin 5 molecules (Gagnoux-Palacios et al. 1996). Similar to laminin 5 produced by wild-type keratinocytes, the recombinant laminin 5 secreted by the reverted LSV5 cells undergoes extracellular maturation, including proteolytic excision of the NH2-terminal domains of the γ2 short arm. In this study, we examined the functional role of the laminin γ2 short arm by complementing the genetic defect of LSV5 cells by transfer of mutant γ2 cDNAs. Expression of the mutant cDNAs demonstrated that all the different recombinant γ2 chains associate with the endogenous laminin α3 and β3 polypeptides to assemble into α3β3γ2 heterotrimers, and that the NH2-terminal domains of the γ2 chain are required for deposition and incorporation of laminin 5 in the ECM.

Our results, for the first time, define a physiologic function for the short arm of the γ2 chain. We demonstrate that mutant laminin 5 molecules lacking the γ2 chain NH2-terminal sequences that are excised in the extracellular processing are found in the spent media of LγC cells, but are not layered on the cell culture substrate. Specifically, deposition of laminin 5 in the ECM produced by the LSV5 keratinocytes requires integrity of the γ2 globular domain IV, whereas preservation of the tightly folded structure of the EGF-like rich domains III and V is not essential. Indeed, transfection of LSV5 cells with γ2 cDNA mutants carrying a deletion within the EGF-like repeats of either domain III or V results in deposition of laminin 5. Consistent with these observations, laminin 5 molecules carrying a deletion of the γ2 domain V EGF-like repeat 2 has been found in the dermal–epidermal junction of a patient with mild epidermolysis bullosa consequent to in-frame skipping of LAMC2 exon 4 (Castiglia, D., P. Posteraro, M. Pinola, C. Angelo, P. Puddu, and A. Zambruno, manuscript submitted for publication). Therefore, removal of EGF-like repeats weakens the functionality of laminin 5, but may not prevent the polarized secretion and deposition of the protein to the basement membrane. Conversely, transfection of mutant pγM, in which the deleted fragment encompasses the γ2 domains III and IV, results in lack of deposition of laminin 5 that correlates well with the epidermolysis bullosa phenotype of the patient carrying this mutation (Pulkkinen et al. 1994). We did not assess whether absent deposition of the mutant γM chain was consequent to lack of secretion. Conversely, by deletion of the proteolytic cleavage site YSGD of the γ2 polypeptide, we show that laminin 5 with an unprocessed γ2 chain (mutant plasmid pγNC) is efficiently layered down and enhances adhesion of LSV5 and wild-type keratinocytes. These finding imply that the extracellular 440-kD form of laminin 5 is an active adhesion ligand and not a mere precursor of the processed 44-kD form.

Basement membrane components are coordinately deposited in the ECM and subsequently assemble to form a supramolecular network sustaining multiple functions, including cell adhesion (Colognato and Yurchenco 2000 and references therein). It has been shown that laminin 5 is one of the first basement membrane components laid down by migrating keratinocytes (Rousselle et al. 1991; Ryan et al. 1994; Lampe et al. 1998). However, the intermolecular interactions of laminin 5 in the basement membrane remain poorly understood. Laminin 5 binds the NC-1 domain of collagen type VII (Cheng et al. 1997; Rousselle et al. 1997) and is found associated with laminin 6 in the skin and laminin 7 in amnion (Rousselle et al. 1991; Gerecke et al. 1994; Champliaud et al. 1996). Utani et al. 1997 have proposed that the ECM component fibulin 2 interacts with the short arm of the mouse γ2 chain. Apart from Phe-202, which is required for the full binding activity of the mouse γ2 chain, the remaining active residues of the consensus sequence mediating interaction between the laminin γ2 chain and fibulin 2 are conserved in humans and other mammals (Table ). We could not demonstrate a direct interaction between the γ2 chain and fibulin 2 in human keratinocytes. In addition, our results show that in mutants γF1 and γF2, substitution of the putative fibulin 2 binding site with alanine residues that modify the nH2-terminal region of the γ2 short arm domain IV does not affect the cleavage of the γ2 chain and deposition of laminin 5. This argues against the participation of this region of the γ2 globular domain IV in the extracellular processing of the γ2 polypeptide (Utani et al. 1997). However, the adhesive function of laminin 5 harboring the mutant γF1 or the γF2 chain is diminished, as attested by the weak resistance to trypsinization of the cells expressing these molecules. The relevance of the γ2 short arm in cell adhesion is confirmed by the fact that substitution of the YSGD site with the amino acids GlyPro, where the proline residue modifies the orientation on the plane of the NH2-terminal domains, also results in efficient deposition of 440-kD laminin 5 molecules exerting a reduced adhesive function. Although further investigations at the molecular level are needed to clarify the mechanisms underlying these observations, it is likely that integrity of the globular domain IV and the correct orientation of the γ2 NH2 terminus determine the appropriate arrangement of laminin 5 in the ECM produced by LSV5 cells and contribute the functional activity of this adhesion ligand.

Laminin 5 purified from mouse epidermis is a mixture of molecules harboring a cleaved (105 kD) or an uncleaved (155 kD) γ2 chain (Aberdam et al. 1994b). In organ-cultured bovine skin, conversion of the 155-kD γ2 chain to 105 kD was not observed, whereas laminin 5 isolated from amnion was found to contain the 105-kD γ2 chain only (Marinkovich et al. 1992). It has been suggested that the proteolytic truncation of the γ2 short arm may facilitate the interaction between the β3 short arm of laminin 5 and other basement membrane components, such as laminins 6 and 7 and collagen type VII, and consequently may activate the adhesion function of laminin 5 (Amano et al. 2000). This hypothesis is not verified in our system, because the mutant laminin 5 molecules harboring a fully processed γ2 short arm are secreted in the culture medium, but are not found in the matrix produced by LSV5 cells. Conversely, we demonstrate that the progressive accumulation of the 440-kD form of laminin 5 on tissue culture plastic and the dermal equivalent of organotypic cultures enhances adhesion of LSV5 cells. Our data confirm previous observations showing that a threshold level of laminin 5 accumulation is required for efficient cell adhesion (Hormia et al. 1995; Gagnoux-Palacios et al. 1996). They also indicate that cell adhesion mediated by laminin 5 is independent of the extracellular processing of the γ2 chain. However, these results do not argue against a role of the processed 400-kD form of laminin 5 in cell adhesion. Proteolysis of the γ2 chain, which may occur after interaction of laminin 5 with laminins 6 and 7 or collagen type VII, could trigger additional or distinct adhesion functions to laminin 5.

Proteolysis regulates ECM assembly, editing the excess ECM components and release of active polypeptides during morphogenesis, growth, tissue repair, and pathological processes (for review see Werb 1997). Proteinases negatively regulate the function of a variety of ECM components and cell surface receptors. It has recently been reported that collagen XVII, a transmembrane component of the hemidesmosomes that contributes to formation of the anchoring filaments, undergoes furin-mediated proteolysis resulting in excision of the large extracellular collagenic domain (Hirako et al. 1998). Integrin β4 also undergoes a proteolytic processing resulting in the cleavage of the cytoplasmic and extracellular domains, probably mediated by calpain and matrilysin (Giancotti et al. 1992; Potts et al. 1994; von Bredow et al. 1997). Therefore, proteolysis of the major components of the anchoring filaments may constitute an effective and rapid mechanism for regulating keratinocyte anchorage. The conversion of laminin 5 into a freely shedding product may thus account for the abundance of processed laminin 5 in spent medium of keratinocyte cell cultures and in body fluids (amniotic, cerebrospinal, and inner ear fluids; Aberdam et al. 1994b).

Proteolytic digestion of the laminin γ2 chain by BMP1 at the physiologic YSGD cleavage site has recently been demonstrated in vitro (Amano et al. 2000). Evidence has also been provided that the 155-kD laminin γ2 chain is cleaved in vitro by the matrix metalloprotease (MMP) MT1-MMP to yield the shortened 105-kD γ2 polypeptide and a 80-kD γ2 chain with a further truncated NH2 terminus (Koshikawa et al. 2000). This observation implies two MT1-MMP cleavage sites on laminin 5. The 80-kD γ2 chain is not found in skin extracts and in cultures of human keratinocytes, whereas the 105-kD γ2 polypeptide and the excised 50-kD NH2-terminal domain are easily detectable. In this study we demonstrate that the γ2 chains missing the tetrapeptide YSGD do not undergo proteolytic steps, which confirms in vitro studies and suggests the involvement of BMP-1 in the processing of laminin 5 at this cleavage site (Amano et al. 2000). On the other hand, although BMP-1–null mice display an ultrastructurally altered cutaneous basement membrane, they do not suffer from epithelial adhesion defects (Suzuki et al. 1996). This may indicate either that the extracellular cleavage of the γ2 chain is not essential to keratinocyte adhesion, or that other proteases, including MT1-MMP, can compensate for the absence of BMP-1 in the extracellular processing of the laminin γ2 chain. Indeed, cleavage of the laminin γ2 chain by MT1-MMP has been associated with the migratory behavior of a variety of transformed epithelial cell lines, and inhibition of MT1-MMP partially downregulates cell migration induced by laminin 5 in tumoral cells. Therefore, MT1-MMP may cleave the γ2 short arm and release the interaction between laminin 5 and the ECM components. Since the proteases of the MMPs family are involved in tissue remodeling in various physiological and pathological conditions, it is tempting to speculate that MT1-MMP cleaves laminin 5 and activates cell migration in pathologic circumstances, whereas BMP-1 governs proteolysis of laminin 5 in a physiologic context.

Several proteolytic fragments of ECM proteins maintain a biological function (Sage 1997). A pentapeptide generated by proteolysis of collagen type I is known to regulate transcription of the col1A1 gene (Katayama et al. 1993), and proliferation of endothelial cells induced by specific growth factors is inhibited by the 16-kD prolactin cleavage fragment (D'Angelo et al. 1995). Laminin γ2 chain and its proteolytic fragments are found at the invasion front of tumors, where they may play positive roles in neoplastic invasion (Pyke et al. 1994, Pyke et al. 1995; Koshikawa et al. 1999). Monomeric laminin γ2 chain is also secreted by carcinoma cells in vitro, but the biological relevance of these observations is unclear (Koshikawa et al. 1999; Maatta et al. 1999; Ono et al. 1999). After proteolytic processing, the γ2 chain short arm is detected in the epithelial basement membrane and in the matrix produced in vitro by normal keratinocytes. Deposition of the recombinant γ2 short arm (γ50) in ECM by cultured keratinocytes confirms the adhesive potential of the polypeptide. Therefore, it is possible that after cleavage from laminin 5 the γ2 short arm sustains interactions with specific basement membranes components and cell receptors and participates in basement membrane assembly. In this regard it has been reported that keratinocyte-specific knock-out of mouse integrin β1 results in disorganization of the cutaneous basement membrane and induces skin blistering that correlates with a reduced immunoreactivity and an altered staining pattern of a pAb antibody directed against the γ2 short arm (Brakebush et al. 2000).

Laminin 5 promotes adhesion, migration, and scattering of several types of cultured cells more efficiently than all the other ECM proteins. We demonstrate that the extracellular deposition of laminin 5 is mediated by the short arm of the γ2 chain that then steers intermolecular interactions with the basement membrane components and promotes cell adhesion. The functional role of the proteolytic processing of the laminin γ2 chain in the formation of the epithelial basement membranes remains unclear. The construction of knock-in mice expressing either a mutant γNC or a truncated γC chain will clarify the specific biological functions of the extracellular forms of laminin 5.

Acknowledgments

We acknowledge A. Spadafora for technical assistance, A. Mastrogiacomo for the protein structural analyses, and A. Charlesworth for critical reading of the manuscript.

This work was supported by grants from EEC BIOMED 2 (BMH4-97-2062), the Programme Hospitalier de Recherche Clinique (France), the DEBRA Foundation (UK), and the Association Francaise contre les Myopathies (Crowthorne, Evry, France).

Footnotes

Abbreviations used in this paper: ECM, extracellular matrix; HA, hemagglutinin; JEB, junctional epidermolysis bullosa; MMP, matrix metalloprotease; pAb, polyclonal antibody.

References

  • Aberdam D., Galliano M.F., Vailly J., Pulkkinen L., Bonifas J., Christiano M., Tryggvason K., Uitto J., Epstein E.H., Jr., Ortonne J.P., Meneguzzi G. Herlitz's junctional epidermolysis bullosa is genetically linked to mutations in the nicein/kalinin (laminin 5) LAMC2 gene Nat. Genet. 61994. 299–304.304a [PubMed]
  • Aberdam D., Aguzzi A., Baudoin C., Galliano M.F., Ortonne J.P., Meneguzzi G. Developmental expression of nicein adhesion protein (laminin-5) subunits suggests multiple morphogenic roles Cell Adhes. Commun. 21994. 115–129.129b [PubMed]
  • Amano S., Scott I.C., Takahara K., Koch M., Gerecke D.R., Keene D.R., Hudson D.L., Nishiyama T., Lee S., Greenspan D.S., Burgeson R.E. Bone morphogenetic protein 1 (BMP-1) is an extracellular processing enzyme of the laminin 5 gamma2 chain. J. Biol. Chem. 2000;275:22728–22735. [PubMed]
  • Baker S.E., Hopkinson S.B., Fitchmun M., Andreason G.L., Frasier F., Plopper G., Quaranta V., Jones J.C.R. Laminin 5 and hemidesmosomesrole of the α3 chain subunit in hemidesmosome stability and assembly. J. Cell Sci. 1996;109:2509–2520. [PubMed]
  • Brakebush C., Grose R., Quondamatteo F., Ramirez A., Jorcano J.L., Pirro A., Svenson M., Herken R., Sasaki T., Topl R., Werner S., Fässel R. Skin and air follicle integrity is crucially dependent on β1 integrin expression on keratinocytes. EMBO (Eur. Mol. Biol. Organ.) J. 2000;19:3990–4003. [PMC free article] [PubMed]
  • Carter W.G., Ryan M.C., Gahr P.J. Epiligrin, a new cell adhesion ligand for integrin α3β1 in epithelial basement membranes. Cell. 1991;65:599–610. [PubMed]
  • Champliaud M.F., Lunstrum G.P., Rousselle P., Nishiyama T., Keene D.R., Burgeson R.E. Human amnion contains a novel laminin variant, laminin 7, which like laminin 6, covalently associates with laminin 5 to promote stable epithelial–stromal attachment. J. Cell Biol. 1996;132:1189–1198. [PMC free article] [PubMed]
  • Chen M., Marinkovich M.P., Veis A., Cai X., Rao C.N., O'Toole E.A., Woodley D.T. Interactions of the amino-terminal noncollagenous (NC1) domain of type VII collagen with extracellular matrix components. A potential role in epidermal-dermal adherence in human skin. J. Biol. Chem. 1997;272:14516–14522. [PubMed]
  • Chen M., Marinkovich M.P., Jones J.C.R., O'Toole E.A., Li Y.-Y., Woodley D.T. NC1 domain of type VII collagen binds to the β3 chain of laminin 5 via a unique subdomain within the fibronectin-like repeats. J. Invest. Dermatol. 1999;112:177–183. [PubMed]
  • Cheng Y.S., Champliaud M.F., Burgeson R.E., Marinkovich M.P., Yurchenco P.D. Self-assembly of laminin isoforms. J. Biol. Chem. 1997;272:31525–31532. [PubMed]
  • Colognato H., Yurchenco P.D. Form and functionthe laminin family of heterotrimers. Dev. Dyn. 2000;218:213–234. [PubMed]
  • D'Angelo G., Struman I., Martial J., Weiner R.I. Activation of mitogen-activated protein kinases by vascular endothelial growth factor and basic fibroblast growth factor in capillary endothelial cells is inhibited by the antiangiogenic factor 16-kDa N-terminal fragment of prolactin. Proc. Natl. Acad. Sci. USA. 1995;92:6374–6378. [PMC free article] [PubMed]
  • Delwel G.O., Hogervorst F., Kuikman I., Paulsson M., Timpl R., Sonnenberg A. Expression and function of the cytoplasmic variants of the integrin alpha 6 subunit in transfected K562 cells. Activation-dependent adhesion and interaction with isoforms of laminin. J. Biol. Chem. 1993;26:25865–25875. [PubMed]
  • DiPersio C.M., Hodivala-Dilke K.M., Jaenisch R., Kreidberg J.A., Hynes R.O. α3β1 integrin is required for normal development of the epidermal basement membrane. J. Cell Biol. 1997;137:729–742. [PMC free article] [PubMed]
  • Gagnoux-Palacios L., Vailly J., Durand-Clement M., Wagner E., Ortonne J.P., Meneguzzi G. Functional re-expression of laminin 5 in laminin-γ2 deficient human keratinocytes modifies cell morphology, motility, and adhesion. J. Biol. Chem. 1996;271:18437–18444. [PubMed]
  • Gerecke D.R., Wagman D.W., Champliaud M.F., Burgeson R.E. The complete primary structure for a novel laminin chain, the laminin B1k chain. J. Biol. Chem. 1994;269:11073–11080. [PubMed]
  • Giancotti F.G., Stepp M.A., Suzuki S., Engvall E., Ruoslahti E. Proteolytic processing of endogenous and recombinant β4 integrin subunit. J. Cell Biol. 1992;118:951–959. [PMC free article] [PubMed]
  • Giannelli G., Falk-Marzillier J., Schiraldi O., Stetler-Stevenson W.G., Quaranta V. Induction of cell migration by matrix metalloprotease-2 cleavage of laminin 5. Science. 1997;277:225–228. [PubMed]
  • Goldfinger L.E., Stack M.S., Jones J.C. Processing of laminin 5 and its functional consequencesrole of plasmin and tissue-type plasminogen activator. J. Cell Biol. 1998;141:255–265. [PMC free article] [PubMed]
  • Hirako Y., Usukura J., Uematsu J., Hashimoto T., Kitajima Y., Owaribe K. Cleavage of BP180, a 180-kDa bullous pemphigoid antigen, yields a 120-kDa collagenous extracellular polypeptide. J. Biol. Chem. 1998;273:9711–9717. [PubMed]
  • Hirosaki T., Mizushima H., Tsubota Y., Moriyama K., Miyazaki K. Structural requirement of carboxyl-terminal globular domains of laminin alpha 3 chain for promotion of rapid cell adhesion and migration by laminin 5. J. Biol. Chem. 2000;275:22495–22502. [PubMed]
  • Hormia M., Falk-Marzillier J., Plopper G., Tamura R.N., Jones J.C., Quaranta V. Rapid spreading and mature hemidesmosome formation in HaCaT keratinocytes induced by incubation with soluble laminin 5r. J. Invest. Dermatol. 1995;105:557–561. [PubMed]
  • Katayama K., Armendariz-Borunda J., Raghow R., Kang A.H., Seyer J.M. A pentapeptide from type I procollagen promotes extracellular matrix production. J. Biol. Chem. 1993;268:9941–9944. [PubMed]
  • Kikkawa Y., Umeda M., Miyazaki K. Marked stimulation of cell adhesion and motility by Ladsin, a laminin-like scatter factor. J. Biochem. 1994;116:862–869. [PubMed]
  • Koshikawa N., Moriyama K., Takamura H., Mizushima H., Nagashima Y., Yanoma S., Miyazaki K. Overexpression of laminin gamma2 chain monomer in invading gastric carcinoma cells. Cancer Res. 1999;21:5596–5601. [PubMed]
  • Koshikawa N., Giannelli G., Cirulli V., Miyazaki K., Quaranta V. Role of cell surface metalloprotease MT1-MMP in epithelial cell migration over laminin 5. J. Cell Biol. 2000;148:615–624. [PMC free article] [PubMed]
  • Lampe P.D., Nguyen B.P., Gil S., Usui M., Olerud J., Takada Y., Carter W. Cellular interaction of integrin alpha3beta1 with laminin 5 promotes gap junctional communication. J. Cell Biol. 1998;143:1735–1747. [PMC free article] [PubMed]
  • Lazarova Z., Yee C., Darling T., Briggaman R.A., Yancey K.B. Passive transfer of anti-laminin 5 antibodies induces subepidermal blisters in neonatal mice. J. Clin. Invest. 1996;98:1509–1518. [PMC free article] [PubMed]
  • Maatta M., Soini Y., Paakko P., Salo S., Tryggvason K., Autio-Harmainen H. Expression of the laminin gamma2 chain in different histological types of lung carcinoma. A study by immunohistochemistry and in situ hybridization. J. Pathol. 1999;188:361–368. [PubMed]
  • Mainiero F., Pepe A., Wary K.K., Spinardi L., Mohammadi M., Schlessinger J., Giancotti F.G. Signal transduction by the α6β4 integrindistinct β4 subunit sites mediate recruitment of Sch/Grb2 and association with the cytoskeleton of hemidesmosomes. EMBO (Eur. Mol. Biol. Organ.) J. 1995;14:4470–4481. [PMC free article] [PubMed]
  • Marinkovich M.P., Lunstrum G.P., Burgeson R.E. The anchoring filament protein kalinin is synthesized and secreted as a high molecular weight precursor. J. Biol. Chem. 1992;267:17900–17906. [PubMed]
  • Masunaga T., Shimizu H., Ishiko A., Tomita Y., Aberdam D., Ortonne J.P., Nishikawa T. Localization of laminin 5 in the epidermal basement membrane. J. Histochem. Cytochem. 1996;44:1223–1230. [PubMed]
  • Miquel C., Gagnoux-Palacios L., Durand-Clément M., Marinkovich P., Ortonne J.P., Meneguzzi G. Establishment and characterization of cell line LSV5 that retains the altered adhesive properties of human junctional epidermolysis bullosa (H-JEB) keratinocytes. Exp. Cell Res. 1996;224:279–290. [PubMed]
  • Mizushima H., Takamura H., Miyagin Y., Kikkawan Y., Yamanakan N., Yasumitsu H., Misugi K., Miyazaki K. Identification of integrin-dependent and -independent cell adhesion domains in COOH-terminal globular region of laminin 5 alpha 3 chain. Cell Growth Differ. 1997;8:979–987. [PubMed]
  • Ono Y., Nakanishi Y., Ino Y., Niki T., Yamada T., Yoshimura K., Saikawa M., Nakajima T., Hirohashi S. Clinicopathologic significance of laminin 5 gamma2 chain expression in squamous cell carcinoma of the tongueimmunohistochemical analysis of 67 lesions. Cancer. 1999;85:2315–2321. [PubMed]
  • O'Toole E.A., Marinkovitch M.P., Hoeffler W., Furthmayr H., Woodley D.T. Laminin 5 inhibits human keratinocyte migration. Exp. Cell Res. 1997;233:330–339. [PubMed]
  • Potts A.J., Croall D.E., Hemler M.E. Proteolytic cleavage of the integrin beta 4 subunit. Exp. Cell Res. 1994;212:2–9. [PubMed]
  • Pulkkinen L., Christiano A.M., Airenne T., Haakana H., Tryggvason K., Uitto J. Mutations in the γ2 chain gene (LAMC2) of kalinin/laminin 5 in the junctional forms of epidermolysis bullosa. Nat. Genet. 1994;6:293–298. [PubMed]
  • Pyke C., Romer J., Kallunki P., Lund L.R., Ralfkiaer E., Tryggvason K. The γ2 chain of kalinin/laminin 5 is preferentially expressed in invading malignant cells in human cancers. Am. J. Pathol. 1994;145:782–791. [PMC free article] [PubMed]
  • Pyke C., Salo S., Ralfkiaer E., Romer J., Dano K., Tryggvason K. Laminin 5 is a marker of invading cancer cells in some human carcinomas and is coexpressed with the receptor for urokinase plasminogen activator in budding cancer cells in colon adenocarcinomas. Cancer Res. 1995;55:4132–4139. [PubMed]
  • Rheinwald J.G., Green H. Serial cultivation of strains of human epidermal keratinocytes; the formation of keratinocytes colonies from single cells. Cell. 1975;6:331–344. [PubMed]
  • Rosdy M., Pisani A., Ortonne J.P. Production of basement membrane components by normal human keratinocytes cultured in the absence of serum and dermal components. Brit. J. Dermatol. 1993;129:227–234. [PubMed]
  • Rousselle P., Aumailley M. Kalinin is more efficient than laminin in promoting adhesion of primary keratinocytes and some other epithelial cells and has a different requirement for integrin receptor. J. Cell Biol. 1994;125:205–214. [PMC free article] [PubMed]
  • Rousselle P., Lunstrum G.P., Keene D.R., Burgeson R.E. Kalininan epithelium-specific basement membrane adhesion molecule that is a component of anchoring filaments. J. Cell Biol. 1991;114:567–576. [PMC free article] [PubMed]
  • Rousselle P., Keene D.R., Ruggiero F., Champliaud M.F., Rest M., Burgeson R.E. Laminin 5 binds the NC-1 domain of type VII collagen. J. Cell Biol. 1997;138:719–728. [PMC free article] [PubMed]
  • Ryan M.C., Tizard R., VanDevanter D.R., Carter W.G. Cloning of the LAMA3 gene encoding the α3 chain of adhesive ligand epiligrin. J. Biol. Chem. 1994;269:22779–22787. [PubMed]
  • Ryan M.C., Lee K., Miyashita Y., Carter W.G. Targeted disruption of the LAMA3 gene in mice reveals abnormalities in survival and late stage differentiation of epithelial cells. J. Cell Biol. 1999;145:1309–1323. [PMC free article] [PubMed]
  • Sage H.E. Piece of eightbioactive fragments of extracellular proteins as regulators of angiogenesis. Trends Cell Biol. 1997;7:182–186. [PubMed]
  • Sonnenberg A., de Melker A.A., Martinez de Velasco A.M., Janssen H., Calafat J., Niessen C.M. Formation of hemidesmosomes in cells of a transformed murine mammary tumor cell line and mechanisms involved in adherence of these cells to laminin and kalinin. J. Cell Sci. 1993;106:1083–1102. [PubMed]
  • Stahl S., Weitzman S., Jones J.C.R. The role of laminin 5 and its receptors in mammary epithelial cell branching morphogenesis. J. Cell Sci. 1997;110:55–63. [PubMed]
  • Suzuki N., Labosky P.A., Furuta Y., Hargett L., Dunn R., Fogo A.B., Takahara K., Peters D.M., Greenspan D.S., Hogan B.L. Failure of ventral body wall closure in mouse embryos lacking a procollagen C-proteinase encoded by Bmp1, a mammalian gene related to Drosophila tolloid. Development. 1996;122:3587–3595. [PubMed]
  • Utani A., Nomizu M., Yamada Y. Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid sequences. J. Biol. Chem. 1997;272:2814–2820. [PubMed]
  • Vailly J., Verrando P., Champliaud M.F., Gerecke D., Wagman D.W., Baudoin C., Aberdam D., Burgeson R., Bauer E., Ortonne J.P. The 100-kDa chain of nicein/kalinin is a laminin B2 chain variant. Eur. J. Biochem. 1994;219:209–218. [PubMed]
  • Vailly J., Gagnoux-Palacios L., Dell'Ambra E., Romero C., Pinola M., Zambruno G., De Luca M., Ortonne J.P., Meneguzzi G. Corrective gene transfer of keratinocytes from patients with junctional epidermolysis bullosa restores assembly of hemidesmosomes in reconstructed epithelia. Gene Ther. 1998;5:1322–1332. [PubMed]
  • Verrando P., Hsi B.L., Yeh C.J., Pisani A., Seryeis N., Ortonne J.P. Monoclonal antibody GB3, a new probe for the study of human basement membranes and hemidesmosomes. Exp. Cell Res. 1987;170:116–128. [PubMed]
  • Vidal F., Baudoin C., Miquel C., Galliano M.F., Christiano A.M., Uitto J., Ortonne J.P., Meneguzzi G. Cloning of the laminin alpha 3 chain gene (LAMA3) and identification of a homozygous deletion in a patient with Herlitz junctional epidermolysis bullosa. Genomics. 1995;30:273–280. [PubMed]
  • von Bredow D.C., Nagle R.B., Bowden G.T., Cress A.E. Cleavage of beta 4 integrin by matrilysin. Exp. Cell Res. 1997;236:341–345. [PubMed]
  • Werb Z. ECM and cell surface proteolysisregulating cellular ecology. Cell. 1997;91:439–442. [PubMed]
  • Yancey K.B., Kirtschig G., Yee C., Lazarova Z. Studies of patients with anti-epiligrin cicatricial pemphigoid. J. Dermatol. 1995;22:829–835. [PubMed]
  • Zhang K., Kramer R.H. Laminin 5 deposition promotes keratinocyte motility. Exp. Cell Res. 1996;227:309–322. [PubMed]

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