Logo of jexpmedHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Exp Med. Apr 1, 1988; 167(4): 1459–1471.
PMCID: PMC2188911

Role of hemolysin for the intracellular growth of Listeria monocytogenes

Abstract

Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 macrophage-like cell line, the CL.7 embryonic mouse fibroblast cell line, and the Henle 407 human epithelial cell line. The hly+ strain grew intracellularly in all of the tissue culture cells with a doubling time of approximately 60 min. In contrast, the hly- mutants failed to grow in the murine-derived tissue culture cells, but retained the ability to grow in the human tissue culture cells examined. Hemolytic-positive revertants were selected after passage of the hly- mutants through monolayers of J774 cells. In each case, the hemolytic revertants possessed the 58-kD polypeptide, were capable of intracellular growth in tissue culture monolayers and were virulent for mice.

Full Text

The Full Text of this article is available as a PDF (848K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Gray ML, Killinger AH. Listeria monocytogenes and listeric infections. Bacteriol Rev. 1966 Jun;30(2):309–382. [PMC free article] [PubMed]
  • Weis J, Seeliger HP. Incidence of Listeria monocytogenes in nature. Appl Microbiol. 1975 Jul;30(1):29–32. [PMC free article] [PubMed]
  • Welshimer HJ, Donker-Voet J. Listeria monocytogenes in nature. Appl Microbiol. 1971 Mar;21(3):516–519. [PMC free article] [PubMed]
  • Listeriosis outbreak associated with Mexican-style cheese--California. MMWR Morb Mortal Wkly Rep. 1985 Jun 21;34(24):357–359. [PubMed]
  • Fleming DW, Cochi SL, MacDonald KL, Brondum J, Hayes PS, Plikaytis BD, Holmes MB, Audurier A, Broome CV, Reingold AL. Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med. 1985 Feb 14;312(7):404–407. [PubMed]
  • Hayes PS, Feeley JC, Graves LM, Ajello GW, Fleming DW. Isolation of Listeria monocytogenes from raw milk. Appl Environ Microbiol. 1986 Feb;51(2):438–440. [PMC free article] [PubMed]
  • Hahn H, Kaufmann SH. The role of cell-mediated immunity in bacterial infections. Rev Infect Dis. 1981 Nov-Dec;3(6):1221–1250. [PubMed]
  • MACKANESS GB. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. [PMC free article] [PubMed]
  • North RJ. The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. J Exp Med. 1970 Sep 1;132(3):521–534. [PMC free article] [PubMed]
  • North RJ. Suppression of cell-mediated immunity to infection by an antimitotic drug. Further evidence that migrant macrophages express immunity. J Exp Med. 1970 Sep 1;132(3):535–545. [PMC free article] [PubMed]
  • Lepay DA, Steinman RM, Nathan CF, Murray HW, Cohn ZA. Liver macrophages in murine listeriosis. Cell-mediated immunity is correlated with an influx of macrophages capable of generating reactive oxygen intermediates. J Exp Med. 1985 Jun 1;161(6):1503–1512. [PMC free article] [PubMed]
  • Rácz P, Tenner K, Mérö E. Experimental Listeria enteritis. I. An electron microscopic study of the epithelial phase in experimental listeria infection. Lab Invest. 1972 Jun;26(6):694–700. [PubMed]
  • Havell EA. Synthesis and secretion of interferon by murine fibroblasts in response to intracellular Listeria monocytogenes. Infect Immun. 1986 Dec;54(3):787–792. [PMC free article] [PubMed]
  • JENKINS EM, NJOKU-OBI AN, ADAMS EW. PURIFICATION OF THE SOLUBLE HEMOLYSINS OF LISTERIA MONOCYTOGENES. J Bacteriol. 1964 Aug;88:418–424. [PMC free article] [PubMed]
  • NJOKU-OBI AN, JENKINS EM, NJOKU-OBI JC, ADAMS J, COVINGTON V. PRODUCTION AND NATURE OF LISTERIA MONOCYTOGENES HEMOLYSINS. J Bacteriol. 1963 Jul;86:1–8. [PMC free article] [PubMed]
  • Gaillard JL, Berche P, Sansonetti P. Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infect Immun. 1986 Apr;52(1):50–55. [PMC free article] [PubMed]
  • Kathariou S, Metz P, Hof H, Goebel W. Tn916-induced mutations in the hemolysin determinant affecting virulence of Listeria monocytogenes. J Bacteriol. 1987 Mar;169(3):1291–1297. [PMC free article] [PubMed]
  • Ralph P, Prichard J, Cohn M. Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity. J Immunol. 1975 Feb;114(2 Pt 2):898–905. [PubMed]
  • Celada A, Gray PW, Rinderknecht E, Schreiber RD. Evidence for a gamma-interferon receptor that regulates macrophage tumoricidal activity. J Exp Med. 1984 Jul 1;160(1):55–74. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Kingdon GC, Sword CP. Biochemical and Immunological Effects of Listeria monocytogenes Hemolysin. Infect Immun. 1970 Apr;1(4):363–372. [PMC free article] [PubMed]
  • Heesemann J, Laufs R. Double immunofluorescence microscopic technique for accurate differentiation of extracellularly and intracellularly located bacteria in cell culture. J Clin Microbiol. 1985 Aug;22(2):168–175. [PMC free article] [PubMed]
  • Hale TL, Bonventre PF. Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infect Immun. 1979 Jun;24(3):879–886. [PMC free article] [PubMed]
  • Hof H. Virulence of different strains of Listeria monocytogenes serovar 1/2a. Med Microbiol Immunol. 1984;173(4):207–218. [PubMed]
  • Berche P, Gaillard JL, Sansonetti PJ. Intracellular growth of Listeria monocytogenes as a prerequisite for in vivo induction of T cell-mediated immunity. J Immunol. 1987 Apr 1;138(7):2266–2271. [PubMed]
  • Weiss AA, Hewlett EL, Myers GA, Falkow S. Tn5-induced mutations affecting virulence factors of Bordetella pertussis. Infect Immun. 1983 Oct;42(1):33–41. [PMC free article] [PubMed]
  • Kingdon GC, Sword CP. Effects of Listeria monocytogenes Hemolysin on Phagocytic Cells and Lysosomes. Infect Immun. 1970 Apr;1(4):356–362. [PMC free article] [PubMed]
  • Geoffroy C, Gaillard JL, Alouf JE, Berche P. Purification, characterization, and toxicity of the sulfhydryl-activated hemolysin listeriolysin O from Listeria monocytogenes. Infect Immun. 1987 Jul;55(7):1641–1646. [PMC free article] [PubMed]
  • Sword CP. Mechanisms of pathogenesis in Listeria monocytogenes infection. I. Influence of iron. J Bacteriol. 1966 Sep;92(3):536–542. [PMC free article] [PubMed]
  • Zachar Z, Savage DC. Microbial interference and colonization of the murine gastrointestinal tract by Listeria monocytogenes. Infect Immun. 1979 Jan;23(1):168–174. [PMC free article] [PubMed]
  • MacDonald TT, Carter PB. Cell-mediated immunity to intestinal infection. Infect Immun. 1980 May;28(2):516–523. [PMC free article] [PubMed]
  • Czuprynski CJ, Henson PM, Campbell PA. Killing of Listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and nonimmune mice. J Leukoc Biol. 1984 Feb;35(2):193–208. [PubMed]
  • Adams DO, Hamilton TA. The cell biology of macrophage activation. Annu Rev Immunol. 1984;2:283–318. [PubMed]
  • Lane FC, Unanue ER. Requirement of thymus (T) lymphocytes for resistance to listeriosis. J Exp Med. 1972 May 1;135(5):1104–1112. [PMC free article] [PubMed]
  • North RJ. Importance of thymus-derived lymphocytes in cell-mediated immunity to infection. Cell Immunol. 1973 Apr;7(1):166–176. [PubMed]
  • Kaufmann SH, Simon MM, Hahn H. Specific Lyt 123 cells are involved in protection against Listeria monocytogenes and in delayed-type hypersensitivity to listerial antigens. J Exp Med. 1979 Oct 1;150(4):1033–1038. [PMC free article] [PubMed]
  • Cheers C, Sandrin MS. Restriction in adoptive transfer of resistance to Listeria monocytogenes. II. Use of congenic and mutant mice show transfer to be H-2K restricted. Cell Immunol. 1983 Jun;78(2):199–205. [PubMed]
  • Zinkernagel RM, Althage A, Adler B, Blanden RV, Davidson WF, Kees U, Dunlop MB, Shreffler DC. H-2 restriction of cell-mediated immunity to an intracellular bacterium: effector T cells are specific for Listeria antigen in association with H-21 region-coded self-markers. J Exp Med. 1977 May 1;145(5):1353–1367. [PMC free article] [PubMed]
  • Kaufmann SH, Hug E, De Libero G. Listeria monocytogenes-reactive T lymphocyte clones with cytolytic activity against infected target cells. J Exp Med. 1986 Jul 1;164(1):363–368. [PMC free article] [PubMed]
  • Emmerling P, Finger H, Hof H. Cell-mediated resistance to infection with Listeria monocytogenes in nude mice. Infect Immun. 1977 Feb;15(2):382–385. [PMC free article] [PubMed]
  • Bancroft GJ, Bosma MJ, Bosma GC, Unanue ER. Regulation of macrophage Ia expression in mice with severe combined immunodeficiency: induction of Ia expression by a T cell-independent mechanism. J Immunol. 1986 Jul 1;137(1):4–9. [PubMed]
  • von Koenig CH, Finger H, Hof H. Failure of killed Listeria monocytogenes vaccine to produce protective immunity. Nature. 1982 May 20;297(5863):233–234. [PubMed]
  • Gaillard JL, Berche P, Mounier J, Richard S, Sansonetti P. In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun. 1987 Nov;55(11):2822–2829. [PMC free article] [PubMed]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...