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J Exp Med. Nov 1, 1986; 164(5): 1785–1790.
PMCID: PMC2188470

Sequence of myosin-crossreactive epitopes of streptococcal M protein

Abstract

Group A streptococcal M proteins contain epitopes that crossreact with sarcolemmal membrane proteins of human myocardium and myosin. In the present study, synthetic peptide copies spanning the entire 197-residue pepsin extracted fragment of type 5 M protein were used to localize the myosin-crossreactive epitopes of the molecule. Peptide 84-116 inhibited by 75% the binding of myosin-crossreactive antibodies evoked by pep M5, as determined by ELISA. Immunoblot inhibition studies confirmed that peptide 84-116 almost totally inhibited the binding of pep M5 antibodies to the heavy chain of human cardiac myosin. None of the remaining synthetic peptides, including peptide 1-35, which contains protective epitopes, inhibited antibodies binding to myosin. Two of three rabbits immunized with peptide 84-116 developed low but significant levels of antibodies crossreactive with myosin. Identification of the primary structures containing tissue- crossreactive as opposed to protective epitopes should not only allow the development of safe and effective M protein vaccines, but may also provide insights into the pathogenesis of rheumatic heart disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • LANCEFIELD RC. Current knowledge of type-specific M antigens of group A streptococci. J Immunol. 1962 Sep;89:307–313. [PubMed]
  • Dale JB, Beachey EH. Protective antigenic determinant of streptococcal M protein shared with sarcolemmal membrane protein of human heart. J Exp Med. 1982 Oct 1;156(4):1165–1176. [PMC free article] [PubMed]
  • Dale JB, Beachey EH. Multiple, heart-cross-reactive epitopes of streptococcal M proteins. J Exp Med. 1985 Jan 1;161(1):113–122. [PMC free article] [PubMed]
  • Dale JB, Beachey EH. Epitopes of streptococcal M proteins shared with cardiac myosin. J Exp Med. 1985 Aug 1;162(2):583–591. [PMC free article] [PubMed]
  • Dale JB, Seyer JM, Beachey EH. Type-specific immunogenicity of a chemically synthesized peptide fragment of type 5 streptococcal M protein. J Exp Med. 1983 Nov 1;158(5):1727–1732. [PMC free article] [PubMed]
  • Beachey EH, Seyer JM. Protective and nonprotective epitopes of chemically synthesized peptides of the NH2-terminal region of type 6 streptococcal M protein. J Immunol. 1986 Mar 15;136(6):2287–2292. [PubMed]
  • Dale JB, Beachey EH. Localization of protective epitopes of the amino terminus of type 5 streptococcal M protein. J Exp Med. 1986 May 1;163(5):1191–1202. [PMC free article] [PubMed]
  • Beachey EH, Campbell GL, Ofek I. Peptic digestion of streptococcal M protein. II. Extraction of M antigen from group A streptococci with pepsin. Infect Immun. 1974 May;9(5):891–896. [PMC free article] [PubMed]
  • Beachey EH, Stollerman GH, Chiang EY, Chiang TM, Seyer JM, Kang AH. Purification and properties of M protein extracted from group A streptococci with pepsin: covalent structure of the amino terminal region of type 24 M antigen. J Exp Med. 1977 Jun 1;145(6):1469–1483. [PMC free article] [PubMed]
  • Beachey EH, Seyer JM, Kang AH. Repeating covalent structure of streptococcal M protein. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3163–3167. [PMC free article] [PubMed]
  • Manjula BN, Acharya AS, Mische SM, Fairwell T, Fischetti VA. The complete amino acid sequence of a biologically active 197-residue fragment of M protein isolated from type 5 group A streptococci. J Biol Chem. 1984 Mar 25;259(6):3686–3693. [PubMed]
  • Seyer JM, Kang AH, Beachey EH. Primary structural similarities between types 5 and 24 M proteins of Streptococcus pyogenes. Biochem Biophys Res Commun. 1980 Jan 29;92(2):546–553. [PubMed]
  • McLachlan AD, Karn J. Periodic features in the amino acid sequence of nematode myosin rod. J Mol Biol. 1983 Mar 15;164(4):605–626. [PubMed]
  • Parry DA. Structure of rabbit skeletal myosin. Analysis of the amino acid sequences of two fragments from the rod region. J Mol Biol. 1981 Dec 5;153(2):459–464. [PubMed]
  • Kavinsky CJ, Umeda PK, Levin JE, Sinha AM, Nigro JM, Jakovcic S, Rabinowitz M. Analysis of cloned mRNA sequences encoding subfragment 2 and part of subfragment 1 of alpha- and beta-myosin heavy chains of rabbit heart. J Biol Chem. 1984 Mar 10;259(5):2775–2781. [PubMed]
  • Pustell J, Kafatos FC. A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):643–655. [PMC free article] [PubMed]
  • Cunningham MW, Hall NK, Krisher KK, Spanier AM. A study of anti-group A streptococcal monoclonal antibodies cross-reactive with myosin. J Immunol. 1986 Jan;136(1):293–298. [PubMed]
  • Manjula BN, Trus BL, Fischetti VA. Presence of two distinct regions in the coiled-coil structure of the streptococcal Pep M5 protein: relationship to mammalian coiled-coil proteins and implications to its biological properties. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1064–1068. [PMC free article] [PubMed]

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