Logo of jexpmedHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Exp Med. 1990 May 1; 171(5): 1779–1784.
PMCID: PMC2187877

Regulation of antigen presentation by acidic pH


The effect of pH on functional association of peptide antigens with APC membranes was investigated by using aldehyde-fixed B cells and class II- restricted T cell hybridomas to assess antigen/MHC complex formation. The results indicated that the rate and extent of functional peptide binding was markedly increased at pH 5.0 as compared with pH 7.3. The pH dependence of binding was preserved after pretreatment of fixed APC with pH 5.0 buffer, suggesting that pH had a direct effect on the interaction of peptide with the APC membrane. Similar results were obtained by using several peptides and I-Ad- and I-Ed-restricted T cells, indicating that pH may be of general importance in regulating the formation of functional antigen/class II MHC complexes.

Full Text

The Full Text of this article is available as a PDF (374K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Babbitt BP, Allen PM, Matsueda G, Haber E, Unanue ER. Binding of immunogenic peptides to Ia histocompatibility molecules. Nature. 317(6035):359–361. [PubMed]
  • Buus S, Sette A, Colon SM, Jenis DM, Grey HM. Isolation and characterization of antigen-Ia complexes involved in T cell recognition. Cell. 1986 Dec 26;47(6):1071–1077. [PubMed]
  • Sadegh-Nasseri S, McConnell HM. A kinetic intermediate in the reaction of an antigenic peptide and I-Ek. Nature. 1989 Jan 19;337(6204):274–276. [PubMed]
  • Roosnek E, Demotz S, Corradin G, Lanzavecchia A. Kinetics of MHC-antigen complex formation on antigen-presenting cells. J Immunol. 1988 Jun 15;140(12):4079–4082. [PubMed]
  • Ceppellini R, Frumento G, Ferrara GB, Tosi R, Chersi A, Pernis B. Binding of labelled influenza matrix peptide to HLA DR in living B lymphoid cells. Nature. 1989 Jun 1;339(6223):392–394. [PubMed]
  • Jensen PE. Stable association of processed antigen with antigen-presenting cell membranes. J Immunol. 1989 Jul 15;143(2):420–425. [PubMed]
  • Kim KJ, Kanellopoulos-Langevin C, Merwin RM, Sachs DH, Asofsky R. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J Immunol. 1979 Feb;122(2):549–554. [PubMed]
  • Ziegler HK, Unanue ER. Decrease in macrophage antigen catabolism caused by ammonia and chloroquine is associated with inhibition of antigen presentation to T cells. Proc Natl Acad Sci U S A. 1982 Jan;79(1):175–178. [PMC free article] [PubMed]
  • Watts TH. T cell activation by preformed, long-lived Ia-peptide complexes. Quantitative aspects. J Immunol. 1988 Dec 1;141(11):3708–3714. [PubMed]
  • Buus S, Sette A, Colon SM, Miles C, Grey HM. The relation between major histocompatibility complex (MHC) restriction and the capacity of Ia to bind immunogenic peptides. Science. 1987 Mar 13;235(4794):1353–1358. [PubMed]
  • Fraser JD. High-affinity binding of staphylococcal enterotoxins A and B to HLA-DR. Nature. 1989 May 18;339(6221):221–223. [PubMed]
  • Nairn R, Spengler ML, Hoffman MD, Solvay MJ, Thomas DW. Macrophage processing of peptide antigens: identification of an antigenic complex. J Immunol. 1984 Dec;133(6):3225–3234. [PubMed]
  • Lakey EK, Margoliash E, Pierce SK. Identification of a peptide binding protein that plays a role in antigen presentation. Proc Natl Acad Sci U S A. 1987 Mar;84(6):1659–1663. [PMC free article] [PubMed]
  • Mellman I, Fuchs R, Helenius A. Acidification of the endocytic and exocytic pathways. Annu Rev Biochem. 1986;55:663–700. [PubMed]
  • Germain RN, Ashwell JD, Lechler RI, Margulies DH, Nickerson KM, Suzuki G, Tou JY. "Exon-shuffling" maps control of antibody- and T-cell-recognition sites to the NH2-terminal domain of the class II major histocompatibility polypeptide A beta. Proc Natl Acad Sci U S A. 1985 May;82(9):2940–2944. [PMC free article] [PubMed]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...