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J Exp Med. May 1, 1990; 171(5): 1797–1802.
PMCID: PMC2187876

Neutrophil-activating properties of the melanoma growth-stimulatory activity

Abstract

Melanoma growth-stimulatory activity (MGSA), a peptide reported to be mitogenic for Hs294T human melanoma cells, has extensive sequence similarity to the neutrophil-activating peptide NAP-1/IL-8, suggesting functional similarities. To test this hypothesis, MGSA was chemically synthesized and tested for its effects on human neutrophils. It was found to induce chemotaxis, exocytosis of elastase, and changes in cytosolic-free calcium to an extent and at concentrations similar to NAP-1/IL-8. However, MGSA was considerably less potent than NAP-1/IL-8 in inducing the respiratory burst. Intradermal injections in rats of MGSA resulted in a massive accumulation of neutrophils. Our data demonstrate that, apart from its growth-stimulatory activity, MGSA is a potent inflammatory agonist with neutrophil-stimulating properties.

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Selected References

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  • Richmond A, Balentien E, Thomas HG, Flaggs G, Barton DE, Spiess J, Bordoni R, Francke U, Derynck R. Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to beta-thromboglobulin. EMBO J. 1988 Jul;7(7):2025–2033. [PMC free article] [PubMed]
  • Anisowicz A, Bardwell L, Sager R. Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7188–7192. [PMC free article] [PubMed]
  • Baggiolini M, Walz A, Kunkel SL. Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest. 1989 Oct;84(4):1045–1049. [PMC free article] [PubMed]
  • Westwick J, Li SW, Camp RD. Novel neutrophil-stimulating peptides. Immunol Today. 1989 May;10(5):146–147. [PubMed]
  • Colditz I, Zwahlen R, Dewald B, Baggiolini M. In vivo inflammatory activity of neutrophil-activating factor, a novel chemotactic peptide derived from human monocytes. Am J Pathol. 1989 Apr;134(4):755–760. [PMC free article] [PubMed]
  • Lindley I, Aschauer H, Seifert JM, Lam C, Brunowsky W, Kownatzki E, Thelen M, Peveri P, Dewald B, von Tscharner V, et al. Synthesis and expression in Escherichia coli of the gene encoding monocyte-derived neutrophil-activating factor: biological equivalence between natural and recombinant neutrophil-activating factor. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9199–9203. [PMC free article] [PubMed]
  • Peveri P, Walz A, Dewald B, Baggiolini M. A novel neutrophil-activating factor produced by human mononuclear phagocytes. J Exp Med. 1988 May 1;167(5):1547–1559. [PMC free article] [PubMed]
  • Clark-Lewis I, Aebersold R, Ziltener H, Schrader JW, Hood LE, Kent SB. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science. 1986 Jan 10;231(4734):134–139. [PubMed]
  • Clark-Lewis I, Hood LE, Kent SB. Role of disulfide bridges in determining the biological activity of interleukin 3. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7897–7901. [PMC free article] [PubMed]
  • Dewald B, Thelen M, Wymann MP, Baggiolini M. Staurosporine inhibits the respiratory burst and induces exocytosis in human neutrophils. Biochem J. 1989 Dec 15;264(3):879–884. [PMC free article] [PubMed]
  • Wymann MP, von Tscharner V, Deranleau DA, Baggiolini M. Chemiluminescence detection of H2O2 produced by human neutrophils during the respiratory burst. Anal Biochem. 1987 Sep;165(2):371–378. [PubMed]
  • Schröder JM, Mrowietz U, Christophers E. Purification and partial biologic characterization of a human lymphocyte-derived peptide with potent neutrophil-stimulating activity. J Immunol. 1988 May 15;140(10):3534–3540. [PubMed]
  • von Tscharner V, Deranleau DA, Baggiolini M. Calcium fluxes and calcium buffering in human neutrophils. J Biol Chem. 1986 Aug 5;261(22):10163–10168. [PubMed]
  • Clark-Lewis I, Lopez AF, To LB, Vadas MA, Schrader JW, Hood LE, Kent SB. Structure-function studies of human granulocyte-macrophage colony-stimulating factor. Identification of residues required for activity. J Immunol. 1988 Aug 1;141(3):881–889. [PubMed]
  • Anisowicz A, Zajchowski D, Stenman G, Sager R. Functional diversity of gro gene expression in human fibroblasts and mammary epithelial cells. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9645–9649. [PMC free article] [PubMed]
  • Wen DZ, Rowland A, Derynck R. Expression and secretion of gro/MGSA by stimulated human endothelial cells. EMBO J. 1989 Jun;8(6):1761–1766. [PMC free article] [PubMed]

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