Logo of jexpmedHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Exp Med. 1984 Aug 1; 160(2): 420–434.
PMCID: PMC2187452

Anti-threonyl-tRNA synthetase, a second myositis-related autoantibody


An autoantibody known as PL-7 was found in the serum of four patients with myositis and one with a systemic lupus erythematosus-like syndrome. The PL-7 antigen is an 80,000 dalton polypeptide that coprecipitates with transfer RNA. In aminoacylation reactions, PL-7 IgG inhibited the charging of tRNA with threonine but had little or no effect on charging with other amino acids. Experimental antibodies raised against purified threonyl-tRNA synthetase recognized the same 80,000 dalton polypeptide, but tRNA was not coprecipitated. We conclude that PL-7 antibody is directed at threonyl-tRNA synthetase, and that different antigenic sites are recognized by the human and experimental autoantibodies. Our findings emphasize the link between myositis and autoimmunity to tRNA-related structures.

Full Text

The Full Text of this article is available as a PDF (2.0M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Tan EM. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982;33:167–240. [PubMed]
  • Harris EN, Gharavi AE, Boey ML, Patel BM, Mackworth-Young CG, Loizou S, Hughes GR. Anticardiolipin antibodies: detection by radioimmunoassay and association with thrombosis in systemic lupus erythematosus. Lancet. 1983 Nov 26;2(8361):1211–1214. [PubMed]
  • Mathews MB, Bernstein RM. Myositis autoantibody inhibits histidyl-tRNA synthetase: a model for autoimmunity. Nature. 1983 Jul 14;304(5922):177–179. [PubMed]
  • Nishikai M, Reichlin M. Heterogeneity of precipitating antibodies in polymyositis and dermatomyositis. Characterization of the Jo-1 antibody system. Arthritis Rheum. 1980 Aug;23(8):881–888. [PubMed]
  • Yoshida S, Akizuki M, Mimori T, Yamagata H, Inada S, Homma M. The precipitating antibody to an acidic nuclear protein antigen, the Jo-1, in connective tissue diseases. A marker for a subset of polymyositis with interstitial pulmonary fibrosis. Arthritis Rheum. 1983 May;26(5):604–611. [PubMed]
  • Bernstein RM, Bunn CC, Hughes GR. Identification of antibodies to acidic antigens by counterimmunoelectrophoresis. Ann Rheum Dis. 1982 Oct;41(5):554–555. [PMC free article] [PubMed]
  • Francoeur AM, Mathews MB. Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6772–6776. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Bonner WM, Laskey RA. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. [PubMed]
  • Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed]
  • Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed]
  • Mathews M, Korner A. Mammalian cell-free protein synthesis directed by viral ribonucleic acid. Eur J Biochem. 1970 Dec;17(2):328–338. [PubMed]
  • Mathews MB, Bernstein RM, Franza BR, Jr, Garrels JI. Identity of the proliferating cell nuclear antigen and cyclin. Nature. 1984 May 24;309(5966):374–376. [PubMed]
  • Gantt JS, Bennett CA, Arfin SM. Increased levels of threonyl-tRNA synthetase in a borrelidin-resistant Chinese hamster ovary cell line. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5367–5370. [PMC free article] [PubMed]
  • Dignam JD, Rhodes DG, Deutscher MP. Purification and structural characterization of rat liver threonyl transfer ribonucleic acid synthetase. Biochemistry. 1980 Oct 28;19(22):4978–4984. [PubMed]
  • Hardin JA, Rahn DR, Shen C, Lerner MR, Wolin SL, Rosa MD, Steitz JA. Antibodies from patients with connective tissue diseases bind specific subsets of cellular RNA-protein particles. J Clin Invest. 1982 Jul;70(1):141–147. [PMC free article] [PubMed]
  • Epstein P, Lidsky M, Reddy R, Tan E, Busch H. Identification of three different anti-4S RNA sera associated with autoimmune disease. Biochem Biophys Res Commun. 1982 Nov 30;109(2):548–555. [PubMed]
  • Matter L, Schopfer K, Wilhelm JA, Nyffenegger T, Parisot RF, De Robertis EM. Molecular characterization of ribonucleoprotein antigens bound by antinuclear antibodies. A diagnostic evaluation. Arthritis Rheum. 1982 Nov;25(11):1278–1283. [PubMed]
  • Nishikai M, Homma M. Circulating autoantibody against human myoglobin in polymyositis. JAMA. 1977 Apr 25;237(17):1842–1844. [PubMed]
  • Lerner MR, Steitz JA. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495–5499. [PMC free article] [PubMed]
  • Bernstein R, Mathews MB. From virus infection to autoantibody formation. Lancet. 1984 Jan 7;1(8367):42–42. [PubMed]
  • Plotz PH. Autoantibodies are anti-idiotype antibodies to antiviral antibodies. Lancet. 1983 Oct 8;2(8354):824–826. [PubMed]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...