• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jexpmedHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Exp Med. Aug 1, 1984; 160(2): 612–617.
PMCID: PMC2187446

Expression of Tac antigen on activated normal human B cells

Abstract

Two-color fluorescence analysis revealed that Tac antigen, which was previously reported to be restricted to T cells, was expressed on a proportion of normal B cells activated by Staphylococcus aureus Cowan I (SAC). Immunoaffinity-purified interleukin 2 (IL-2) induced the proliferation of SAC-activated B cells, and the proliferation was completely inhibited by anti-Tac antibody, which blocked the membrane binding and action of IL-2. These results suggest that an IL-2 receptor system is directly involved in the B cell immune response.

Full Text

The Full Text of this article is available as a PDF (457K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Uchiyama T, Broder S, Waldmann TA. A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. J Immunol. 1981 Apr;126(4):1393–1397. [PubMed]
  • Leonard WJ, Depper JM, Uchiyama T, Smith KA, Waldmann TA, Greene WC. A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptor. Nature. 1982 Nov 18;300(5889):267–269. [PubMed]
  • Robb RJ, Greene WC. Direct demonstration of the identity of T cell growth factor binding protein and the Tac antigen. J Exp Med. 1983 Oct 1;158(4):1332–1337. [PMC free article] [PubMed]
  • Robb RJ, Munck A, Smith KA. T cell growth factor receptors. Quantitation, specificity, and biological relevance. J Exp Med. 1981 Nov 1;154(5):1455–1474. [PMC free article] [PubMed]
  • Korsmeyer SJ, Greene WC, Cossman J, Hsu SM, Jensen JP, Neckers LM, Marshall SL, Bakhshi A, Depper JM, Leonard WJ, et al. Rearrangement and expression of immunoglobulin genes and expression of Tac antigen in hairy cell leukemia. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4522–4526. [PMC free article] [PubMed]
  • Muraguchi A, Fauci AS. Proliferative responses of normal human B lymphocytes. Development of an assay system for human B cell growth factor (BCGF). J Immunol. 1982 Sep;129(3):1104–1108. [PubMed]
  • Oi VT, Glazer AN, Stryer L. Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J Cell Biol. 1982 Jun;93(3):981–986. [PMC free article] [PubMed]
  • Smith KA, Favata MF, Oroszlan S. Production and characterization of monoclonal antibodies to human interleukin 2: strategy and tactics. J Immunol. 1983 Oct;131(4):1808–1815. [PubMed]
  • Parker DC. Separable helper factors support B cell proliferation and maturation to Ig secretion. J Immunol. 1982 Aug;129(2):469–474. [PubMed]
  • Liebson HJ, Marrack P, Kappler J. B cell helper factors. II. Synergy among three helper factors in the response of T cell- and macrophage-depleted B cells. J Immunol. 1982 Oct;129(4):1398–1402. [PubMed]
  • Howard M, Matis L, Malek TR, Shevach E, Kell W, Cohen D, Nakanishi K, Paul WE. Interleukin 2 induces antigen-reactive T cell lines to secrete BCGF-I. J Exp Med. 1983 Dec 1;158(6):2024–2039. [PMC free article] [PubMed]
  • Depper JM, Leonard WJ, Robb RJ, Waldmann TA, Greene WC. Blockade of the interleukin-2 receptor by anti-Tac antibody: inhibition of human lymphocyte activation. J Immunol. 1983 Aug;131(2):690–696. [PubMed]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...