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J Exp Med. Sep 1, 1983; 158(3): 718–730.
PMCID: PMC2187101

Idiotypic cross-reactions of monoclonal human lupus autoantibodies

Abstract

Idiotypic cross-reactions were evaluated in 60 polynucleotide-binding monoclonal lupus autoantibodies produced by human-human hybridomas that were derived from seven unrelated patients with SLE. Three antiidiotype reagents were prepared by immunization of rabbits or a mouse with monoclonal autoantibodies from two patients. Binding of the three reagents to their corresponding idiotypes was inhibited by one or more polynucleotides, an indication that the antiidiotypes reacted with the variable regions of the autoantibodies. Each antiidiotype appeared to detect a different idiotypic determinant. Of the 60 monoclonal autoantibodies tested, 40 reacted in one or more competitive immunoassays; 15 reacted with one antiidiotype, 10 reacted with two antiidiotypes and 15 reacted with three antiidiotypes. A monoclonal antiidiotype reagent cross-reacted with autoantibodies from six of the seven patients. The idiotypic cross-reactions of immunoglobulins from unrelated patients suggest that the autoantibodies are derived from related families of germ line genes that are expressed by patients with SLE.

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Selected References

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  • Andrzejewski C, Jr, Stollar BD, Lalor TM, Schwartz RS. Hybridoma autoantibodies to DNA. J Immunol. 1980 Mar;124(3):1499–1502. [PubMed]
  • Hahn BH, Ebling F, Freeman S, Clevinger B, Davie J. Production of monoclonal murine antibodies to DNA by somatic cell hybrids. Arthritis Rheum. 1980 Aug;23(8):942–945. [PubMed]
  • Tron F, Charron D, Bach JF, Talal N. Establishment and characterization of a murine hybridoma secreting monoclonal anti-DNA autoantibody. J Immunol. 1980 Dec;125(6):2805–2809. [PubMed]
  • Eilat D, Asofsky R, Laskov R. A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA. J Immunol. 1980 Feb;124(2):766–768. [PubMed]
  • Andrzejewski C, Jr, Rauch J, Lafer E, Stollar BD, Schwartz RS. Antigen-binding diversity and idiotypic cross-reactions among hybridoma autoantibodies to DNA. J Immunol. 1981 Jan;126(1):226–231. [PubMed]
  • Pisetsky DS, Caster SA. Binding specificity of a monoclonal anti-DNA antibody. Mol Immunol. 1982 May;19(5):645–650. [PubMed]
  • Koike T, Nagasawa R, Nagata N, Shirai T. Specificity of mouse hybridoma antibodies to DNA. Immunol Lett. 1982 Feb;4(2):93–97. [PubMed]
  • Shoenfeld Y, Rauch J, Massicotte H, Datta SK, André-Schwartz J, Stollar BD, Schwartz RS. Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas. N Engl J Med. 1983 Feb 24;308(8):414–420. [PubMed]
  • Lafer EM, Rauch J, Andrzejewski C, Jr, Mudd D, Furie B, Furie B, Schwartz RS, Stollar BD. Polyspecific monoclonal lupus autoantibodies reactive with both polynucleotides and phospholipids. J Exp Med. 1981 Apr 1;153(4):897–909. [PMC free article] [PubMed]
  • Koike T, Tomioka H, Kumagai A. Antibodies cross-reactive with DNA and cardiolipin in patients with systemic lupus erythematosus. Clin Exp Immunol. 1982 Nov;50(2):298–302. [PMC free article] [PubMed]
  • Shoenfeld Y, Hsu-Lin SC, Gabriels JE, Silberstein LE, Furie BC, Furie B, Stollar BD, Schwartz RS. Production of autoantibodies by human-human hybridomas. J Clin Invest. 1982 Jul;70(1):205–208. [PMC free article] [PubMed]
  • Solomon G, Schiffenbauer J, Keiser HD, Diamond B. Use of monoclonal antibodies to identify shared idiotypes on human antibodies to native DNA from patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1983 Feb;80(3):850–854. [PMC free article] [PubMed]
  • Lamoyi E, Estess P, Capra JD, Nisonoff A. Heterogeneity of an intrastrain cross-reactive idiotype associated with anti-p-azophenylarsonate antibodies of A/J mice. J Immunol. 1980 Jun;124(6):2834–2840. [PubMed]
  • Marion TN, Lawton AR, 3rd, Kearney JF, Briles DE. Anti-DNA autoantibodies in (NZB X NZW)F1 mice are clonally heterogeneous, but the majority share a common idiotype. J Immunol. 1982 Feb;128(2):668–674. [PubMed]
  • Tron F, Le Guern C, Cazenave PA, Bach JF. Intrastrain recurrent idiotypes among anti-DNA antibodies of (NZB x NZW)F1 hybrid mice. Eur J Immunol. 1982 Sep;12(9):761–766. [PubMed]
  • Rauch J, Murphy E, Roths JB, Stollar BD, Schwartz RS. A high frequency idiotypic marker of anti-DNA autoantibodies in MRL-Ipr/Ipr mice. J Immunol. 1982 Jul;129(1):236–241. [PubMed]
  • Datta SK, Stollar BD, Schwartz RS. Normal mice express idiotypes related to autoantibody idiotypes of lupus mice. Proc Natl Acad Sci U S A. 1983 May;80(9):2723–2727. [PMC free article] [PubMed]
  • Willims RC, Jr, Kunkel HG, Capra JD. Antigenic specificities related to the cold agglutinin activity of gamma M globulins. Science. 1968 Jul 26;161(3839):379–381. [PubMed]
  • Kunkel HG, Agnello V, Joslin FG, Winchester RJ, Capra JD. Cross-idiotypic specificity among monoclonal IgM proteins with anti- -globulin activity. J Exp Med. 1973 Feb 1;137(2):331–342. [PMC free article] [PubMed]
  • Geltner D, Franklin EC, Frangione B. Antiidiotypic activity in the IgM fractions of mixed cryoglobulins. J Immunol. 1980 Oct;125(4):1530–1535. [PubMed]
  • Lefvert AK. Anti-idiotypic antibodies against the receptor antibodies in myasthenia gravis. Scand J Immunol. 1981;13(5):493–497. [PubMed]
  • Tachovsky TG, Sandberg-Wollheim M, Baird LG. Rabbit anti-human CSF IgG. I. Characterization of anti-idiotype antibodies produced against MS CSF and detection of cross-reactive idiotypes in several MS CSF. J Immunol. 1982 Aug;129(2):764–770. [PubMed]
  • Kennedy RC, Dreesman GR. Common idiotypic determinant associated with human antibodies to hepatitis B surface antigen. J Immunol. 1983 Jan;130(1):385–389. [PubMed]

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