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J Bacteriol. Apr 1979; 138(1): 109–117.
PMCID: PMC218245

Change from Homo- to Heterolactic Fermentation by Streptococcus lactis Resulting from Glucose Limitation in Anaerobic Chemostat Cultures

Abstract

Lactic streptococci, classically regarded as homolactic fermenters of glucose and lactose, became heterolactic when grown with limiting carbohydrate concentrations in a chemostat. At high dilution rates (D) with excess glucose present, about 95% of the fermented sugar was converted to l-lactate. However, as D was lowered and glucose became limiting, five of the six strains tested changed to a heterolactic fermentation such that at D = 0.1 h−1 as little as 1% of the glucose was converted to l-lactate. The products formed after this phenotypic change in fermentation pattern were formate, acetate, and ethanol. The level of lactate dehydrogenase, which is dependent upon ketohexose diphosphate for activity, decreased as fermentation became heterolactic with Streptococcus lactis ML3. Transfer of heterolactic cells from the chemostat to buffer containing glucose resulted in the nongrowing cells converting nearly 80% of the glucose to l-lactate, indicating that fine control of enzyme activity is an important factor in the fermentation change. These nongrowing cells metabolizing glucose had elevated (ca. twofold) intracellular fructose 1,6-diphosphate concentrations ([FDP]in) compared with those in the glucose-limited heterolactic cells in the chemostat. [FDP]in was monitored during the change in fermentation pattern observed in the chemostat when glucose became limiting. Cells converting 95 and 1% of the glucose to l-lactate contained 25 and 10 mM [FDP]in, respectively. It is suggested that factors involved in the change to heterolactic fermentation include both [FDP]in and the level of lactate dehydrogenase.

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Selected References

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  • Bissett DL, Anderson RL. Lactose and D-galactose metabolism in group N streptococci: presence of enzymes for both the D-galactose 1-phosphate and D-tagatose 6-phosphate pathways. J Bacteriol. 1974 Jan;117(1):318–320. [PMC free article] [PubMed]
  • Brown WV, Collins EB. End products and fermentation balances for lactic streptococci grown aerobically on low concentrations of glucose. Appl Environ Microbiol. 1977 Jan;33(1):38–42. [PMC free article] [PubMed]
  • Brown AT, Wittenberger CL. Fructose-1,6-diphosphate-dependent lactate dehydrogenase from a cariogenic streptococcus: purification and regulatory properties. J Bacteriol. 1972 May;110(2):604–615. [PMC free article] [PubMed]
  • Collins LB, Thomas TD. Pyruvate kinase of Streptococcus lactis. J Bacteriol. 1974 Oct;120(1):52–58. [PMC free article] [PubMed]
  • Crow VL, Pritchard GG. Fructose 1,6-diphosphate-activated L-lactate dehydrogenase from Streptococcus lactis: kinetic properties and factors affecting activation. J Bacteriol. 1977 Jul;131(1):82–91. [PMC free article] [PubMed]
  • Demko GM, Blanton SJ, Benoit RE. Heterofermentative carbohydrate metabolism of lactose-impaired mutants of Streptococcus lactis. J Bacteriol. 1972 Dec;112(3):1335–1345. [PMC free article] [PubMed]
  • de Vries W, Kapteijn WM, van der Beek EG, Stouthamer AH. Molar growth yields and fermentation balances of Lactobacillus casei L3 in batch cultures and in continuous cultures. J Gen Microbiol. 1970 Nov;63(3):333–345. [PubMed]
  • Ellwood DC, Hunter JR, Longyear VM. Growth of Streptococcus mutans in a chemostat. Arch Oral Biol. 1974 Aug;19(8):659–664. [PubMed]
  • Ellwood DC, Tempest DW. Control of teichoic acid and teichuronic acid biosyntheses in chemostat cultures of Bacillus subtilis var. niger. Biochem J. 1969 Jan;111(1):1–5. [PMC free article] [PubMed]
  • Harrison DE, Maitra PK. Control of respiration and metabolism in growing Klebsiella aerogenes. The role of adenine nucleotides. Biochem J. 1969 May;112(5):647–656. [PMC free article] [PubMed]
  • HARVEY RJ, COLLINS EB. ROLES OF CITRATE AND ACETOIN IN THE METABOLISM OF STREPTOCOCCUS DIACETILACTIS. J Bacteriol. 1963 Dec;86:1301–1307. [PMC free article] [PubMed]
  • Herbert D, Kornberg HL. Glucose transport as rate-limiting step in the growth of Escherichia coli on glucose. Biochem J. 1976 May 15;156(2):477–480. [PMC free article] [PubMed]
  • Herbert D, Phipps PJ, Tempest DW. The chemostat: design and instrumentation. Lab Pract. 1965 Oct;14(10):1150–1161. [PubMed]
  • Jonas HA, Anders RF, Jago GR. Factors affecting the activity of the lactate dehydrognease of Streptococcus cremoris. J Bacteriol. 1972 Aug;111(2):397–403. [PMC free article] [PubMed]
  • Lindmark DG, Paolella P, Wood NP. The pyruvate formate-lyase system of Streptococcus faecalis. I. Purification and properties of the formate-pyruvate exchange enzyme. J Biol Chem. 1969 Jul 10;244(13):3605–3612. [PubMed]
  • McDonald IJ. Occurence of lactose-negative mutants in chemostat cultures of lactic streptococci. Can J Microbiol. 1975 Mar;21(3):245–251. [PubMed]
  • McKay LL, Baldwin KA. Altered metabolism in a Streptococcus lactis C2 mutant deficient in lactic dehydrogenase. J Dairy Sci. 1974 Feb;57(2):181–186. [PubMed]
  • Niven DF, Collins PA, Knowles CJ. Adenylate energy charge during batch culture of Beneckea natriegens. J Gen Microbiol. 1977 Jan;98(1):95–108. [PubMed]
  • PLATT TB, FOSTER EM. Products of glucose metabolism by homofermentative streptococci under anaerobic conditions. J Bacteriol. 1958 Apr;75(4):453–459. [PMC free article] [PubMed]
  • Sanwal BD. Allosteric controls of amphilbolic pathways in bacteria. Bacteriol Rev. 1970 Mar;34(1):20–39. [PMC free article] [PubMed]
  • Thomas TD. Tagatose-1, 6-diphosphate activation of lactate dehydrogenase from Streptococcus cremoris. Biochem Biophys Res Commun. 1975 Apr 21;63(4):1035–1042. [PubMed]
  • Thomas TD. Regulation of lactose fermentation in group N streptococci. Appl Environ Microbiol. 1976 Oct;32(4):474–478. [PMC free article] [PubMed]
  • Thompson J. In vivo regulation of glycolysis and characterization of sugar: phosphotransferase systems in Streptococcus lactis. J Bacteriol. 1978 Nov;136(2):465–476. [PMC free article] [PubMed]
  • Thompson J, Thomas TD. Phosphoenolpyruvate and 2-phosphoglycerate: endogenous energy source(s) for sugar accumulation by starved cells of Streptococcus lactis. J Bacteriol. 1977 May;130(2):583–595. [PMC free article] [PubMed]
  • Yamada T, Carlsson J. Regulation of lactate dehydrogenase and change of fermentation products in streptococci. J Bacteriol. 1975 Oct;124(1):55–61. [PMC free article] [PubMed]

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