• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jexpmedHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Exp Med. Jul 1, 1977; 146(1): 218–229.
PMCID: PMC2180735

Dengue viruses and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supporting in vitro infection

Abstract

Studies were made on the identity of human and monkey mononuclear leukocytes permissive to antibody-enhanced dengue 2 virus (D2V) infection. In cultures of peripheral blood leukocytes (PBL) inoculated immediately after separation, it was concluded that only mononuclear phagocytes support dengue infection. This is based upon observations that D2V-permissive cells were resistant to 1,200 rads, were both plastic adherent and nonadherent, were removed when passed through nylon wool columns in 10 percent fetal bovine serum or 100 percent autologous serum, and were destroyed by incubation with 100 μg/ml particulate silica. On direct immunofluorescence staining, perinuclear dengue antigen was visualized at 24 h, becoming maximal at 60 h. Antigen-containing cells had ample cytoplasm, ruffled cytoplasmic membrane, and 73 percent were actively phagocytic. As further evidence of the infection of mononuclear phagocytes, antibody-enhanced D2V replication was observed in bone marrow cultures from five of five rhesus monkeys, but not in cell cultures of spleen, thymus, or lymph nodes prepared from the same animals. It is hypothesized that dengue virus complexed with non-neutralizing antibody is internalized by immune phagocytosis in a mononuclear phagocyte with a defective virus-destroying mechanism. Dengue permissiveness may depend upon cellular immaturity since bone marrow leukocytes could be infected even when held for 4 days before infection while PBL held for this time decreased in permissiveness. In vitro antibody-dependent infection of mononuclear phagocytes should prove useful as a model for study of immunopathologic mechanisms in human dengue.

Full Text

The Full Text of this article is available as a PDF (796K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Halstead SB, O'Rourke EJ. Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody. J Exp Med. 1977 Jul 1;146(1):201–217. [PMC free article] [PubMed]
  • MacLennan IC, Loewi G, Harding B. The role of immunoglobulins in lymphocyte-mediated cell damage, in vitro. I. Comparison of the effects of target cell specific antibody and normal serum factors on cellular damage by immune and non-immune lymphocytes. Immunology. 1970 Mar;18(3):397–404. [PMC free article] [PubMed]
  • Dickler HB. Studies of the human lymphocyte receptor for heat-aggregated or antigen-complexed immunoglobulin. J Exp Med. 1974 Aug 1;140(2):508–522. [PMC free article] [PubMed]
  • Koller CA, King GW, Hurtubise PE, Sagone AL, LoBuglio AF. Characterization of glass adherent human mononuclear cells. J Immunol. 1973 Nov;111(5):1610–1612. [PubMed]
  • Halstead SB, Chow JS, Marchette NJ. Immunological enhancement of dengue virus replication. Nat New Biol. 1973 May 2;243(122):24–26. [PubMed]
  • Sung JS, Diwan AR, Falkler WA, Jr, Yang HY, Halstead SB. Dengue carrier culture and antigen production in human lymphoblastoid lines. Intervirology. 1975;5(3-4):137–149. [PubMed]
  • Theofilopoulos AN, Brandt WE, Russell PK, Dixon FT. Replication of dengue-2 virus in cultured human lymphoblastoid cells and subpopulations of human peripheral leukocytes. J Immunol. 1976 Sep;117(3):953–961. [PubMed]
  • Horwitz DA. The development of macrophages from large mononuclear cells in the blood of patients with inflammatory disease. J Clin Invest. 1972 Apr;51(4):760–768. [PMC free article] [PubMed]
  • VOLKMAN A, GOWANS JL. THE ORIGIN OF MACROPHAGES FROM BONE MARROW IN THE RAT. Br J Exp Pathol. 1965 Feb;46:62–70. [PMC free article] [PubMed]
  • van Furth R, Cohn ZA. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. [PMC free article] [PubMed]
  • Goud TJ, Schotte C, van Furth R. Identification and characterization of the monoblast in mononuclear phagocyte colonies grown in vitro. J Exp Med. 1975 Nov 1;142(5):1180–1199. [PMC free article] [PubMed]
  • van Furth R, Diesselhoff-Den Dulk MM. The kinetics of promonocytes and monocytes in the bone marrow. J Exp Med. 1970 Oct 1;132(4):813–828. [PMC free article] [PubMed]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...