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J Bacteriol. Aug 1983; 155(2): 761–767.
PMCID: PMC217748

Construction of a mobilizable Yersinia enterocolitica virulence plasmid.

Abstract

Virulence of Yersinia enterocolitica O:8 is associated with pO:8, a 42-megadalton plasmid. We constructed a mobilizable pO:8 derivative by successive in vitro and in vivo genetic manipulations. The in vitro constructed hybrid molecule pRK290B8-5 consisting of the mobilizable vector pRK290B and a 2.9-megadalton BamHI fragment of pO:8 was conjugally transferred to a Y. enterocolitica strain of serotype O:8 which harbored the virulence plasmid pO:8. From Yersinia transconjugants, a cointegrate was isolated which apparently formed by homologous recombination between the two component plasmids. The cointegrate was mobilized into plasmidless Y. enterocolitica strains of different serotypes. The transconjugants of serotype O:8 were found to express all four plasmid-associated phenotypes: (i) mouse lethality (Ml), (ii) conjunctivitis provocation in the guinea pig eye (Con), (iii) calcium requirement for growth at 37 degrees C (Mox), and (iv) agglutinogens (Ag8). The transconjugants of serotype O:3 expressed the phenotypes Con, Mox, and Ag8 but were nonlethal for mice (Ml-). The transconjugants of serotype O:5 remained avirulent for mice (Ml-) and for the guinea pig eye (Con-) but expressed the phenotypes Mox and Ag8. These data show that the virulence plasmid is probably not functionally interchangeable within different serotypes of Y. enterocolitica.

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Selected References

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  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Kay BA, Wachsmuth K, Gemski P. New virulence-associated plasmid in Yersinia enterocolitica. J Clin Microbiol. 1982 Jun;15(6):1161–1163. [PMC free article] [PubMed]
  • Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. [PMC free article] [PubMed]
  • Dagert M, Ehrlich SD. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. [PubMed]
  • Ditta G, Stanfield S, Corbin D, Helinski DR. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347–7351. [PMC free article] [PubMed]
  • Dugaiczyk A, Boyer HW, Goodman HM. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol. 1975 Jul 25;96(1):171–184. [PubMed]
  • Gemski P, Lazere JR, Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. [PMC free article] [PubMed]
  • Hoopes BC, McClure WR. Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Res. 1981 Oct 24;9(20):5493–5504. [PMC free article] [PubMed]
  • Keggins KM, Duvall EJ, Lovett PS. Recombination between compatible plasmids containing homologous segments requires the Bacillus subtilis recE gene product. J Bacteriol. 1978 May;134(2):514–520. [PMC free article] [PubMed]
  • Knapp W, Lysy J, Knapp C, Stille W, Goll U. Enterale Infektionen beim Menschen durch Yersinia enterocolitica und ihre Diagnose. Infection. 1973;1(2):113–125. [PubMed]
  • Lee WH, Smith RE, Damaré JM, Harris ME, Johnston RW. Evaluation of virulence test procedures for Yersinia enterocolitica recovered from foods. J Appl Bacteriol. 1981 Jun;50(3):529–539. [PubMed]
  • Maniatis T, Hardison RC, Lacy E, Lauer J, O'Connell C, Quon D, Sim GK, Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. [PubMed]
  • Peterson BC, Hashimoto H, Rownd RH. Cointegrate formation between homologous plasmids in Escherichia coli. J Bacteriol. 1982 Sep;151(3):1086–1094. [PMC free article] [PubMed]
  • Portnoy DA, Moseley SL, Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. [PMC free article] [PubMed]
  • Ratnam S, Mercer E, Picco B, Parsons S, Butler R. A nosocomial outbreak of diarrheal disease due to Yersinia enterocolitica serotype 0:5, biotype 1. J Infect Dis. 1982 Feb;145(2):242–247. [PubMed]
  • Robinson MK, Bennett PM, Falkow S, Dodd HM. Isolation of a temperature-sensitive derivative of RP1. Plasmid. 1980 May;3(3):343–347. [PubMed]
  • SERENY B. Experimental shigella keratoconjunctivitis; a preliminary report. Acta Microbiol Acad Sci Hung. 1955;2(3):293–296. [PubMed]
  • Zink DL, Feeley JC, Wells JG, Vanderzant C, Vickery JC, Roof WD, O'Donovan GA. Plasmid-mediated tissue invasiveness in Yersinia enterocolitica. Nature. 1980 Jan 10;283(5743):224–226. [PubMed]

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