Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jun 1983; 154(3): 1145–1152.
PMCID: PMC217585

Spontaneous deletions and flanking regions of the chromosomally inherited hemolysin determinant of an Escherichia coli O6 strain.


The hemolytic Escherichia coli strain 536 (O6) propagates spontaneous hemolysin-negative mutants at relatively high rates (10(-3) to 10(-4)). One type of mutant (type I) lacks both secreted (external) and periplasmic (internal) hemolysin activity (Hlyex-/Hlyin-) and in addition shows no mannose-resistant hemagglutination (Mrh-), whereas the other type (type II) is Hlyex-/Hlyin+ and Mrh+. The genetic determinants for hemolysin production (hly) and for mannose-resistant hemagglutination (mrh) of this strain are located on the chromosome. Hybridization experiments with DNA probes specific for various parts of the hly determinant reveal that mutants of type I have lost the total hly determinant, whereas those of type II lack only part of the hlyB that is essential for transport of hemolysin across the outer membrane. Using a probe that contains the end sequence of the plasmid pHly152-encoded hly determinant (adjacent to hlyB), we determined that a related sequence flanks also the hlyB-distal end of the chromosomal hly determinant of E. coli 536. In addition several other similar or even identical sequences are found in the vicinity of the hlyC- and the hlyB-distal ends of both the chromosomal and the plasmid hly determinants.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Berger H, Hacker J, Juarez A, Hughes C, Goebel W. Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli. J Bacteriol. 1982 Dec;152(3):1241–1247. [PMC free article] [PubMed]
  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Cohen SN, Chang AC, Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. [PMC free article] [PubMed]
  • de la Cruz F, Müller D, Ortiz JM, Goebel W. Hemolysis determinant common to Escherichia coli hemolytic plasmids of different incompatibility groups. J Bacteriol. 1980 Aug;143(2):825–833. [PMC free article] [PubMed]
  • Evans DJ, Jr, Evans DG, Höhne C, Noble MA, Haldane EV, Lior H, Young LS. Hemolysin and K antigens in relation to serotype and hemagglutination type of Escherichia coli isolated from extraintestinal infections. J Clin Microbiol. 1981 Jan;13(1):171–178. [PMC free article] [PubMed]
  • Evans DJ, Jr, Evans DG, Young LS, Pitt J. Hemagglutination typing of Escherichia coli: definition of seven hemagglutination types. J Clin Microbiol. 1980 Aug;12(2):235–242. [PMC free article] [PubMed]
  • Goebel W, Hedgpeth J. Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli. J Bacteriol. 1982 Sep;151(3):1290–1298. [PMC free article] [PubMed]
  • Goebel W, Schrempf H. Isolation and characterization of supercoiled circular deoxyribonucleic acid from beta-hemolytic strains of Escherichia coli. J Bacteriol. 1971 May;106(2):311–317. [PMC free article] [PubMed]
  • Green CP, Thomas VL. Hemagglutination of human type O erythrocytes, hemolysin production, and serogrouping of Escherichia coli isolates from patients with acute pyelonephritis, cystitis, and asymptomatic bacteriuria. Infect Immun. 1981 Jan;31(1):309–315. [PMC free article] [PubMed]
  • Grinsted J, Bennett PM, Higginson S, Richmond MH. Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives. Mol Gen Genet. 1978 Nov 9;166(3):313–320. [PubMed]
  • Hagberg L, Jodal U, Korhonen TK, Lidin-Janson G, Lindberg U, Svanborg Edén C. Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections. Infect Immun. 1981 Feb;31(2):564–570. [PMC free article] [PubMed]
  • Kleckner N. Transposable elements in prokaryotes. Annu Rev Genet. 1981;15:341–404. [PubMed]
  • Lederberg EM, Cohen SN. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974 Sep;119(3):1072–1074. [PMC free article] [PubMed]
  • Minshew BH, Jorgensen J, Counts GW, Falkow S. Association of hemolysin production, hemagglutination of human erythrocytes, and virulence for chicken embryos of extraintestinal Escherichia coli isolates. Infect Immun. 1978 Apr;20(1):50–54. [PMC free article] [PubMed]
  • Noegel A, Rdest U, Goebel W. Determination of the functions of hemolytic plasmid pHly152 of Escherichia coli. J Bacteriol. 1981 Jan;145(1):233–247. [PMC free article] [PubMed]
  • Noegel A, Rdest U, Springer W, Goebel W. Plasmid cistrons controlling synthesis and excretion of the exotoxin alpha-haemolysin of Escherichia coli. Mol Gen Genet. 1979 Oct 1;175(3):343–350. [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Smith HW, Halls S. The transmissible nature of the genetic factor in Escherichia coli that controls haemolysin production. J Gen Microbiol. 1967 Apr;47(1):153–161. [PubMed]
  • Springer W, Goebel W. Synthesis and secretion of hemolysin by Escherichia coli. J Bacteriol. 1980 Oct;144(1):53–59. [PMC free article] [PubMed]
  • So M, Heffron F, McCarthy BJ. The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature. 1979 Feb 8;277(5696):453–456. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Starlinger P, Saedler H. IS-elements in microorganisms. Curr Top Microbiol Immunol. 1976;75:111–152. [PubMed]
  • Eden CS, Eriksson B, Hanson LA. Adhesion of Escherichia coli to human uroepithelial cells in vitro. Infect Immun. 1977 Dec;18(3):767–774. [PMC free article] [PubMed]
  • Welch RA, Dellinger EP, Minshew B, Falkow S. Haemolysin contributes to virulence of extra-intestinal E. coli infections. Nature. 1981 Dec 17;294(5842):665–667. [PubMed]
  • van den Bosch JF, Postma P, de Graaff J, MacLaren DM. Haemolysis by urinary Escherichia coli and virulence in mice. J Med Microbiol. 1981 Aug;14(3):321–331. [PubMed]
  • Yamamoto T, Yokota T. Escherichia coli heat-labile enterotoxin genes are flanked by repeated deoxyribonucleic acid sequences. J Bacteriol. 1981 Feb;145(2):850–860. [PMC free article] [PubMed]
  • Zabala JC, de la Cruz F, Ortiz JM. Several copies of the same insertion sequence are present in alpha-hemolytic plasmids belonging to four different incompatibility groups. J Bacteriol. 1982 Jul;151(1):472–476. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...