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J Bacteriol. May 1983; 154(2): 1002–1004.
PMCID: PMC217559

Cloning of genes that complement yeast hexokinase and glucokinase mutants.


Genes complementing the glucose-negative fructose-negative Saccharomyces cerevisiae triple mutant strain (hxkl hxk2 glk1), which lacks hexokinase PI, hexokinase PII, and glucokinase, were obtained from a pool of yeast DNA in the multicopy plasmid YEp13.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bisson LF, Fraenkel DG. Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1730–1734. [PMC free article] [PubMed]
  • Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed]
  • Clifton D, Weinstock SB, Fraenkel DG. Glycolysis mutants in Saccharomyces cerevisiae. Genetics. 1978 Jan;88(1):1–11. [PMC free article] [PubMed]
  • Entian KD, Mecke D. Genetic evidence for a role of hexokinase isozyme PII in carbon catabolite repression in Saccharomyces cerevisiae. J Biol Chem. 1982 Jan 25;257(2):870–874. [PubMed]
  • Gancedo JM, Clifton D, Fraenkel DG. Yeast hexokinase mutants. J Biol Chem. 1977 Jul 10;252(13):4443–4444. [PubMed]
  • Kawasaki G, Fraenkel DG. Cloning of yeast glycolysis genes by complementation. Biochem Biophys Res Commun. 1982 Oct 15;108(3):1107–1122. [PubMed]
  • Lobo Z, Maitra PK. Genetics of yeast hexokinase. Genetics. 1977 Aug;86(4):727–744. [PMC free article] [PubMed]
  • Lobo Z, Maitra PK. Resistance to 2-deoxyglucose in yeast: a direct selection of mutants lacking glucose-phosphorylating enzymes. Mol Gen Genet. 1977 Dec 9;157(3):297–300. [PubMed]
  • Lobo Z, Maitra PK. Physiological role of glucose-phosphorylating enzymes in Saccharomyces cerevisiae. Arch Biochem Biophys. 1977 Aug;182(2):639–645. [PubMed]

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