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J Bacteriol. May 1983; 154(2): 793–802.
PMCID: PMC217531

Regulation of alternate peripheral pathways of glucose catabolism during aerobic and anaerobic growth of Pseudomonas aeruginosa.

Abstract

Glucose may be converted to 6-phosphogluconate by alternate pathways in Pseudomonas aeruginosa. Glucose is phosphorylated to glucose-6-phosphate, which is oxidized to 6-phosphogluconate during anaerobic growth when nitrate is used as respiratory electron acceptor. Mutant cells lacking glucose-6-phosphate dehydrogenase are unable to catabolize glucose under these conditions. The mutant cells utilize glucose as effectively as do wild-type cells in the presence of oxygen; under these conditions, glucose is utilized via direct oxidation to gluconate, which is converted to 6-phosphogluconate. The membrane-associated glucose dehydrogenase activity was not formed during anaerobic growth with glucose. Gluconate, the product of the enzyme, appeared to be the inducer of the gluconate transport system, gluconokinase, and membrane-associated gluconate dehydrogenase. 6-Phosphogluconate is probably the physiological inducer of glucokinase, glucose-6-phosphate dehydrogenase, and the dehydratase and aldolase of the Entner-Doudoroff pathway. Nitrate-linked respiration is required for the anaerobic uptake of glucose and gluconate by independently regulated transport systems in cells grown under denitrifying conditions.

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Selected References

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  • Blevins WT, Feary TW, Phibbs PV., Jr 6-Phosphogluconate dehydratase deficiency in pleiotropic carbohydrate-negative mutant strains of Pseudomonas aeruginosa. J Bacteriol. 1975 Mar;121(3):942–949. [PMC free article] [PubMed]
  • Guymon LF, Eagon RG. Transport of glucose, gluconate, and methyl alpha-D-glucoside by Pseudomonas aeruginosa. J Bacteriol. 1974 Mar;117(3):1261–1269. [PMC free article] [PubMed]
  • Hederstedt L, Holmgren E, Rutberg L. Characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of Bacillus subtilis with the nonionic detergent Triton X-100. J Bacteriol. 1979 May;138(2):370–376. [PMC free article] [PubMed]
  • Holmgren E, Hederstedt L, Rutberg L. Role of heme in synthesis and membrane binding of succinic dehydrogenase in Bacillus subtilis. J Bacteriol. 1979 May;138(2):377–382. [PMC free article] [PubMed]
  • Hunt JC, Phibbs PV., Jr Failure of Pseudomonas aeruginosa to form membrane-associated glucose dehydrogenase activity during anaerobic growth with nitrate. Biochem Biophys Res Commun. 1981 Oct 30;102(4):1393–1399. [PubMed]
  • Hylemon PB, Krieg NR, Phibbs PV., Jr Transport and catabolism of D-fructose by Spirillum itersomii. J Bacteriol. 1974 Jan;117(1):144–150. [PMC free article] [PubMed]
  • Hylemon PB, Phibbs PV., Jr Independent regulation of hexose catabolizing enzymes and glucose transport activity in Pseudomonas aeruginosa. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1041–1048. [PubMed]
  • Lessie TG, Berka T, Zamanigian S. Pseudomonas cepacia mutants blocked in the direct oxidative pathway of glucose degradation. J Bacteriol. 1979 Jul;139(1):323–325. [PMC free article] [PubMed]
  • Lessie T, Neidhardt FC. Adenosine triphosphate-linked control of Pseudomonas aeruginosa glucose-6-phosphate dehydrogenase. J Bacteriol. 1967 Apr;93(4):1337–1345. [PMC free article] [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • MacGregor CH. Anaerobic cytochrome b1 in Escherichia coli: association with and regulation of nitrate reductase. J Bacteriol. 1975 Mar;121(3):1111–1116. [PMC free article] [PubMed]
  • MacGregor CH. Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli. J Bacteriol. 1975 Mar;121(3):1117–1121. [PMC free article] [PubMed]
  • Midgley M, Dawes EA. The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa. Biochem J. 1973 Feb;132(2):141–154. [PMC free article] [PubMed]
  • Mitchell CG, Dawes EA. The role of oxygen in the regulation of glucose metabolism, transport and the tricarboxylic acid cycle in Pseudomonas aeruginosa. J Gen Microbiol. 1982 Jan;128(1):49–59. [PubMed]
  • Phibbs PV, Jr, Eagon RG. Transport and phosphorylation of glucose, fructose, and mannitol by Pseudomonas aeruginosa. Arch Biochem Biophys. 1970 Jun;138(2):470–482. [PubMed]
  • Phibbs PV, Jr, Feary TW, Blevins WT. Pyruvate carboxylase deficiency in pleiotropic carbohydrate-negative mutant strains of Pseudomonas aeruginosa. J Bacteriol. 1974 Jun;118(3):999–1009. [PMC free article] [PubMed]
  • Phibbs PV, Jr, McCowen SM, Feary TW, Blevins WT. Mannitol and fructose catabolic pathways of Pseudomonas aeruginosa carbohydrate-negative mutants and pleiotropic effects of certain enzyme deficiencies. J Bacteriol. 1978 Feb;133(2):717–728. [PMC free article] [PubMed]
  • Roberts BK, Midgley M, Dawes EA. The metabolism of 2-oxogluconate by Pseudomonas aeruginosa. J Gen Microbiol. 1973 Oct;78(2):319–329. [PubMed]
  • Showe MK, DeMoss JA. Localization and regulation of synthesis of nitrate reductase in Escherichia coli. J Bacteriol. 1968 Apr;95(4):1305–1313. [PMC free article] [PubMed]
  • Sinclair PR, White DC. Effect of nitrate, fumarate, and oxygen on the formation of the membrane-bound electron transport system of Haemophilus parainfluenzae. J Bacteriol. 1970 Feb;101(2):365–372. [PMC free article] [PubMed]
  • Spangler WJ, Gilmour CM. Biochemistry of nitrate respiration in Pseudomonas stutzeri. I. Aerobic and nitrate respiration routes of carbohydrate catabolism. J Bacteriol. 1966 Jan;91(1):245–250. [PMC free article] [PubMed]
  • STOKES FN, CAMPBELL JJR. The oxidation of glucose and gluconic acid by dried cells of Pseudomonas aeruginosa. Arch Biochem. 1951 Jan;30(1):121–125. [PubMed]
  • Tiwari NP, Campbell JJ. Enzymatic control of the metabolic activity of Pseudomonas aeruginosa grown in glucose or succinate media. Biochim Biophys Acta. 1969 Dec 30;192(3):395–401. [PubMed]
  • Vicente M, Cánovas JL. Regulation of the glucolytic enzymes in Pseudomonas putida. Arch Mikrobiol. 1973 Oct 4;93(1):53–64. [PubMed]
  • Whiting PH, Midgley M, Dawes EA. The regulation of transport of glucose, gluconate and 2-oxogluconate and of glucose catabolism in Pseudomonas aeruginosa. Biochem J. 1976 Mar 15;154(3):659–668. [PMC free article] [PubMed]
  • Whiting PH, Midgley M, Dawes EA. The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in Pseudomonas aeruginosa. J Gen Microbiol. 1976 Feb;92(2):304–310. [PubMed]
  • Williams DR, Rowe JJ, Romero P, Eagon RG. Denitrifying Pseudomonas aeruginosa: some parameters of growth and active transport. Appl Environ Microbiol. 1978 Aug;36(2):257–263. [PMC free article] [PubMed]

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