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J Bacteriol. Jul 1979; 139(1): 107–114.
PMCID: PMC216833

Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium.

Abstract

Generally nonchemotactic mutants of Escherichia coli and Salmonella typhimurium were analyzed by interspecies complementation tests to determine the functional correspondence between the che genes of these two organisms. The E. coli che region was introduced into Salmonella recipients by means of a series of F-prime elements. Wild-type che genes of E. coli F'420 complemented all che mutants of Salmonella except cheS, cheV, and a subclass of cheU. A series of tester episomes carrying E. coli che mutations were then used to determine which E. coli che function was responsible for the complementation of each Salmonella che defect. By this method, the following correspondences were determined: cheAE and chePS,cheWE, and cheWS, cheXE and cheRS, cheYE and cheQS, cheCE and cheUS, cheBE and cheXS, and cheZE and cheTS. (The subscripts E and S refer to E. coli and S. typhimurium genes, respectively.) In some tests, especially those involving the last two pairs of genes, poor complementation was observed between noncorresponding genes. A model explaining these observations in terms of subunit interactions is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adler J. Chemotaxis in bacteria. Annu Rev Biochem. 1975;44:341–356. [PubMed]
  • Armstrong JB, Adler J. Complementation of nonchemotactic mutants of Escherichia coli. Genetics. 1969 Jan;61(1):61–66. [PMC free article] [PubMed]
  • Aswad D, Koshland DE., Jr Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants. J Mol Biol. 1975 Sep 15;97(2):225–235. [PubMed]
  • Bachmann BJ, Low KB, Taylor AL. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. [PMC free article] [PubMed]
  • Collins AL, Stocker BA. Salmonella typhimurium mutants generally defective in chemotaxis. J Bacteriol. 1976 Dec;128(3):754–765. [PMC free article] [PubMed]
  • Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. [PubMed]
  • Khan S, Macnab RM, DeFranco AL, Koshland DE., Jr Inversion of a behavioral response in bacterial chemotaxis: explanation at the molecular level. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4150–4154. [PMC free article] [PubMed]
  • Kort EN, Goy MF, Larsen SH, Adler J. Methylation of a membrane protein involved in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3939–3943. [PMC free article] [PubMed]
  • Koshland DE., Jr A response regulator model in a simple sensory system. Science. 1977 Jun 3;196(4294):1055–1063. [PubMed]
  • Macnab RM. Bacterial motility and chemotaxis: the molecular biology of a behavioral system. CRC Crit Rev Biochem. 1978;5(4):291–341. [PubMed]
  • Parkinson JS. Data processing by the chemotaxis machinery of Escherichia coli. Nature. 1974 Nov 22;252(5481):317–319. [PubMed]
  • Parkinson JS. cheA, cheB, and cheC genes of Escherichia coli and their role in chemotaxis. J Bacteriol. 1976 May;126(2):758–770. [PMC free article] [PubMed]
  • Parkinson JS. Behavioral genetics in bacteria. Annu Rev Genet. 1977;11:397–414. [PubMed]
  • Parkinson JS. Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis. J Bacteriol. 1978 Jul;135(1):45–53. [PMC free article] [PubMed]
  • Parkinson JS, Parker SR. Interaction of the cheC and cheZ gene products is required for chemotactic behavior in Escherichia coli. Proc Natl Acad Sci U S A. 1979 May;76(5):2390–2394. [PMC free article] [PubMed]
  • Rubik BA, Koshland DE., Jr Potentiation, desensitization, and inversion of response in bacterial sensing of chemical stimuli. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2820–2824. [PMC free article] [PubMed]
  • Silverman M, Simon M. Genetic analysis of bacteriophage Mu-induced flagellar mutants in Escherichia coli. J Bacteriol. 1973 Oct;116(1):114–122. [PMC free article] [PubMed]
  • Silverman M, Simon M. Identification of polypeptides necessary for chemotaxis in Escherichia coli. J Bacteriol. 1977 Jun;130(3):1317–1325. [PMC free article] [PubMed]
  • Springer WR, Koshland DE., Jr Identification of a protein methyltransferase as the cheR gene product in the bacterial sensing system. Proc Natl Acad Sci U S A. 1977 Feb;74(2):533–537. [PMC free article] [PubMed]
  • Stock JB, Koshland DE., Jr A protein methylesterase involved in bacterial sensing. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3659–3663. [PMC free article] [PubMed]
  • VOGEL HJ, BONNER DM. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed]
  • Warrick HM, Taylor BL, Koshland DE., Jr Chemotactic mechanism of Salmonella typhimurium: preliminary mapping and characterization of mutants. J Bacteriol. 1977 Apr;130(1):223–231. [PMC free article] [PubMed]

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