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J Bacteriol. Nov 1981; 148(2): 734–735.
PMCID: PMC216264

Improved generalized transducing bacteriophage for Caulobacter crescentus.


Caulobacter phage phi Cr30T, a temperature-sensitive derivative of the lytic, generalized transducing phage phi Cr30, was isolated as a double temperature-sensitive recombinant in a cross between phi Cr30ts1 and phi Cr30ts2, phi Cr30T mediated generalized transduction of Caulobacter crescentus at frequencies comparable to those of phi Cr30 and eliminated the requirement for irradiation of transducing lysates to prevent killing of transductants on the plate.

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Selected References

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  • Contreras I, Bender RA, Mansour J, Henry S, Shapiro L. Caulobacter cresentus mutant defective in membrane phospholipid synthesis. J Bacteriol. 1979 Nov;140(2):612–619. [PMC free article] [PubMed]
  • Ely B, Amarasinghe AB, Bender RA. Ammonia assimilation and glutamate formation in Caulobacter crescentus. J Bacteriol. 1978 Jan;133(1):225–230. [PMC free article] [PubMed]
  • Ely B, Johnson RC. Generalized Transduction in CAULOBACTER CRESCENTUS. Genetics. 1977 Nov;87(3):391–399. [PMC free article] [PubMed]
  • George J, Devoret R, Radman M. Indirect ultraviolet-reactivation of phage lambda. Proc Natl Acad Sci U S A. 1974 Jan;71(1):144–147. [PMC free article] [PubMed]
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  • Poindexter JS. The caulobacters: ubiquitous unusual bacteria. Microbiol Rev. 1981 Mar;45(1):123–179. [PMC free article] [PubMed]
  • Scott JR. Genetic studies on bacteriophage P1. Virology. 1968 Dec;36(4):564–574. [PubMed]
  • Shapiro L. Differentiation in the Caulobacter cell cycle. Annu Rev Microbiol. 1976;30:377–407. [PubMed]

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