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J Bacteriol. Oct 1981; 148(1): 265–273.
PMCID: PMC216189

Role of sulA and sulB in filamentation by lon mutants of Escherichia coli K-12.

Abstract

Cells containing the pleiotropic Escherichia coli mutation lon filament extensively and die after exposure to ultraviolet light. Outside suppressors of the ultraviolet sensitivity, called sul, have previously been described at two loci; these mutations reverse the ultraviolet sensitivity of lon strains but do not affect the mucoidal or degradation defect of these strains. An isogenic set of strains carrying combinations of lon, sulA, and sulI was constructed, and their behavior during normal growth and after ultraviolet treatment was studied. sulA mutations had no detectable phenotype in lon+ cells; the lon sulA strains filamented transiently after ultraviolet irradiation, as did lon+ sul+ cells. We found that the sulB mutation, which alters cell morphology and slows recovery from transient filamentation after ultraviolet treatment, was epistatic to both lon and sulA. Whereas sulA mutations were recessive to the wild-type allele, sulB was partially dominant. The simplest model to account for our observations is that sulA and lon participate in a pathway of filamentation independent of that which produces transient filamentation in wild-type strains; sulB product may be the target of sulA action and may play a role in normal cell division.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Apte BN, Rhodes H, Zipser D. Mutation blocking the specific degradation of reinitiation polypeptides in E. coli. Nature. 1975 Sep 25;257(5524):329–331. [PubMed]
  • Bukhari AI, Zipser D. Mutants of Escherichia coli with a defect in the degradation of nonsense fragments. Nat New Biol. 1973 Jun 20;243(129):238–241. [PubMed]
  • Falkinham JO., 3rd Gene lon and plasmid inheritance in Escherichia coli K-12. J Bacteriol. 1979 Sep;139(3):1054–1057. [PMC free article] [PubMed]
  • Feiss M, Adyha S, Court DL. Isolation of plaque-forming, galactose-transducing strains of phage lambda. Genetics. 1972 Jun;71(2):189–206. [PMC free article] [PubMed]
  • Gayda RC, Yamamoto LT, Markovitz A. Second-site mutations in capR (lon) strains of Escherichia coli K-12 that prevent radiation sensitivity and allow bacteriophage lambda to lysogenize. J Bacteriol. 1976 Sep;127(3):1208–1216. [PMC free article] [PubMed]
  • George J, Castellazzi M, Buttin G. Prophage induction and cell division in E. coli. III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif. Mol Gen Genet. 1975 Oct 22;140(4):309–332. [PubMed]
  • Gottesman S, Gottesman M, Shaw JE, Pearson ML. Protein degradation in E. coli: the lon mutation and bacteriophage lambda N and cII protein stability. Cell. 1981 Apr;24(1):225–233. [PubMed]
  • Gottesman S, Zipser D. Deg phenotype of Escherichia coli lon mutants. J Bacteriol. 1978 Feb;133(2):844–851. [PMC free article] [PubMed]
  • HOWARD-FLANDERS P, SIMSON E, THERIOT L. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12. Genetics. 1964 Feb;49:237–246. [PMC free article] [PubMed]
  • Huisman O, D'Ari R. An inducible DNA replication-cell division coupling mechanism in E. coli. Nature. 1981 Apr 30;290(5809):797–799. [PubMed]
  • Huisman O, D'Ari R, George J. Inducible sfi dependent division inhibition in Escherichia coli. Mol Gen Genet. 1980;177(4):629–636. [PubMed]
  • Huisman O, D'Ari R, George J. Further characterization of sfiA and sfiB mutations in Escherichia coli. J Bacteriol. 1980 Oct;144(1):185–191. [PMC free article] [PubMed]
  • Johnson BF. Fine structure mapping and properties of mutations suppressing the lon mutation in Escherichia coli K-12 and B strains. Genet Res. 1977 Dec;30(3):273–286. [PubMed]
  • Johnson BF, Greenberg J. Mapping of sul, the suppressor of lon in Escherichia coli. J Bacteriol. 1975 May;122(2):570–574. [PMC free article] [PubMed]
  • Shineberg B, Zipser D. The ion gene and degradation of beta-galactosidase nonsense fragments. J Bacteriol. 1973 Dec;116(3):1469–1471. [PMC free article] [PubMed]
  • Takano T. Bacterial mutants defective in plasmid formation: requirement for the lon + allele. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1469–1473. [PMC free article] [PubMed]
  • Walker JR, Ussery CL, Allen JS. Bacterial cell division regulation: lysogenization of conditional cell division lon - mutants of Escherichia coli by bacteriophage. J Bacteriol. 1973 Mar;113(3):1326–1332. [PMC free article] [PubMed]
  • Witkin EM. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. [PMC free article] [PubMed]

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