• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Sep 1986; 167(3): 981–985.
PMCID: PMC215968

Characteristics of sterol uptake in Saccharomyces cerevisiae.

Abstract

A Saccharomyces cerevisiae sterol auxotroph, FY3 (alpha hem1 erg7 ura), was used to probe the characteristics of sterol uptake in S. cerevisiae. The steady-state cellular concentration of free sterol at the late exponential phase of growth could be adjusted within a 10-fold range by varying the concentration of exogenously supplied sterol. When cultured on 1 microgram of sterol ml-1, the cells contained a minimal cellular free-cholesterol concentration of 0.85 nmol/mg (dry weight) and were termed sterol depleted. When cultured on 11 micrograms of sterol ml-1 or more, the cells contained a maximal cellular free-cholesterol concentration of 6.8 nmol/mg (dry weight) and were termed free sterol saturated. Cells with free-sterol concentrations below the maximal level were capable of accumulating free sterol from the medium. The capacity of the cells for cholesterol uptake was inversely proportional to the initial intracellular concentration. The uptake of sterol was shown to be a nonactive process that is independent of cellular energy sources or viability. The intracellular transport of sterol for esterification is not sensitive to anti-microtubule agents.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • ANDREASEN AA, STIER TJB. Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. J Cell Physiol. 1953 Feb;41(1):23–36. [PubMed]
  • Bottema CD, Rodriguez RJ, Parks LW. Influence of sterol structure on yeast plasma membrane properties. Biochim Biophys Acta. 1985 Mar 14;813(2):313–320. [PubMed]
  • Chambaut-Guérin AM, Muller P, Rossignol B. Microtubules and protein secretion in rat lacrimal glands. Relationship between colchicine binding and its inhibitory effect on the intracellular transport of proteins. J Biol Chem. 1978 Jun 10;253(11):3870–3876. [PubMed]
  • Downing JF, Burrows LS, Bard M. The isolation of two mutants of Saccharomyces cerevisiae which demonstrate increased activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Biochem Biophys Res Commun. 1980 Jun 16;94(3):974–979. [PubMed]
  • Gollub EG, Liu KP, Dayan J, Adlersberg M, Sprinson DB. Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene. J Biol Chem. 1977 May 10;252(9):2846–2854. [PubMed]
  • Hata S, Nishino T, Komori M, Katsuki H. Involvement of cytochrome P-450 in delta 22-desaturation in ergosterol biosynthesis of yeast. Biochem Biophys Res Commun. 1981 Nov 16;103(1):272–277. [PubMed]
  • Lewis TA, Taylor FR, Parks LW. Involvement of heme biosynthesis in control of sterol uptake by Saccharomyces cerevisiae. J Bacteriol. 1985 Jul;163(1):199–207. [PMC free article] [PubMed]
  • Low C, Rodriguez RJ, Parks LW. Modulation of yeast plasma membrane composition of a yeast sterol auxotroph as a function of exogenous sterol. Arch Biochem Biophys. 1985 Aug 1;240(2):530–538. [PubMed]
  • Molzahn SW, Woods RA. Polyene resistance and the isolation of sterol mutants in Saccharomyces cerevisiae. J Gen Microbiol. 1972 Sep;72(2):339–348. [PubMed]
  • Nes WR, Dhanuka IC, Pinto WJ. Evidence for facilitated transport in the absorption of sterols by Saccharomyces cerevisiae. Lipids. 1986 Jan;21(1):102–106. [PubMed]
  • Nes WR, Sekula BC, Nes WD, Adler JH. The functional importance of structural features of ergosterol in yeast. J Biol Chem. 1978 Sep 10;253(17):6218–6225. [PubMed]
  • Parks LW, Bottema CD, Rodriguez RJ, Lewis TA. Yeast sterols: yeast mutants as tools for the study of sterol metabolism. Methods Enzymol. 1985;111:333–346. [PubMed]
  • Parks LW, McLean-Bowen C, Taylor FR, Hough S. Sterols in yeast subcellular fractions. Lipids. 1978 Oct;13(10):730–735. [PubMed]
  • Patzelt C, Brown D, Jeanrenaud B. Inhibitory effect of colchicine on amylase secretion by rat parotid glands. Possible localization in the Golgi area. J Cell Biol. 1977 Jun;73(3):578–593. [PMC free article] [PubMed]
  • Pinto WJ, Lozano R, Nes WR. Inhibition of sterol biosynthesis by ergosterol and cholesterol in Saccharomyces cerevisiae. Biochim Biophys Acta. 1985 Aug 22;836(1):89–95. [PubMed]
  • Pinto WJ, Nes WR. Stereochemical specificity for sterols in Saccharomyces cerevisiae. J Biol Chem. 1983 Apr 10;258(7):4472–4476. [PubMed]
  • Quinlan RA, Pogson CI, Gull K. The influence of the microtubule inhibitor, methyl benzimidazol-2-yl-carbamate (MBC) on nuclear division and the cell cycle in Saccharomyces cerevisiae. J Cell Sci. 1980 Dec;46:341–352. [PubMed]
  • Rodriguez RJ, Low C, Bottema CD, Parks LW. Multiple functions for sterols in Saccharomyces cerevisiae. Biochim Biophys Acta. 1985 Dec 4;837(3):336–343. [PubMed]
  • Rodriguez RJ, Parks LW. Structural and physiological features of sterols necessary to satisfy bulk membrane and sparking requirements in yeast sterol auxotrophs. Arch Biochem Biophys. 1983 Sep;225(2):861–871. [PubMed]
  • Skipski VP, Smolowe AF, Sullivan RC, Barclay M. Separation of lipid classes by thin-layer chromatography. Biochim Biophys Acta. 1965 Oct 4;106(2):386–396. [PubMed]
  • Taketani S, Nishino T, Katsuki H. Characterization of sterol-ester synthetase in Saccharomyces cerevisiae. Biochim Biophys Acta. 1979 Oct 26;575(1):148–155. [PubMed]
  • Taylor FR, Parks LW. Metabolic interconversion of free sterols and steryl esters in Saccharomyces cerevisiae. J Bacteriol. 1978 Nov;136(2):531–537. [PMC free article] [PubMed]
  • Taylor FR, Parks LW. Adaptation of Saccharomyces cerevisiae to growth on cholesterol: selection of mutants defective in the formation of lanosterol. Biochem Biophys Res Commun. 1980 Aug 29;95(4):1437–1445. [PubMed]
  • Taylor FR, Parks LW. An assessment of the specificity of sterol uptake and esterification in Saccharomyces cerevisiae. J Biol Chem. 1981 Dec 25;256(24):13048–13054. [PubMed]
  • Trocha PJ, Sprinson DB. Location and regulation of early enzymes of sterol biosynthesis in yeast. Arch Biochem Biophys. 1976 May;174(1):45–51. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links