• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jun 1985; 162(3): 1322–1324.
PMCID: PMC215926

Regulation of nitrogen fixation in Rhodospirillum rubrum grown under dark, fermentative conditions.


Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenting cells was reactivated by an endogenous membrane-bound, Mn2+-dependent activating enzyme that was interchangeable with the activating enzyme isolated from photosynthetic membranes.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (508K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Carithers RP, Yoch DC, Arnon DI. Two forms of nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. J Bacteriol. 1979 Feb;137(2):779–789. [PMC free article] [PubMed]
  • Gotto JW, Yoch DC. Regulation of Rhodospirillum rubrum nitrogenase activity. Properties and interconversion of active and inactive Fe protein. J Biol Chem. 1982 Mar 25;257(6):2868–2873. [PubMed]
  • Gotto JW, Yoch DC. Purification and Mn2+ activation of Rhodospirillum rubrum nitrogenase activating enzyme. J Bacteriol. 1982 Nov;152(2):714–721. [PMC free article] [PubMed]
  • Gotto JW, Yoch DC. Regulation of nitrogenase activity by covalent modification in Chromatium vinosum. Arch Microbiol. 1985 Feb;141(1):40–43. [PubMed]
  • Kanemoto RH, Ludden PW. Effect of ammonia, darkness, and phenazine methosulfate on whole-cell nitrogenase activity and Fe protein modification in Rhodospirillum rubrum. J Bacteriol. 1984 May;158(2):713–720. [PMC free article] [PubMed]
  • Ludden PW, Burris RH. Activating factor for the iron protein of nitrogenase from Rhodospirillum rubrum. Science. 1976 Oct 22;194(4263):424–426. [PubMed]
  • Ludden PW, Burris RH. Removal of an adenine-like molecule during activation of dinitrogenase reductase from Rhodospirillum rubrum. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6201–6205. [PMC free article] [PubMed]
  • Madigan MT, Wall JD, Gest H. Dark anaerobic dinitrogen fixation by a photosynthetic microorganism. Science. 1979 Jun 29;204(4400):1429–1430. [PubMed]
  • Neilson AH, Nordlund S. Regulation of nitrogenase synthesis in intact cells of Rhodospirillum rubrum: inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine. J Gen Microbiol. 1975 Nov;91(1):53–62. [PubMed]
  • Nordlund S, Eriksson U. Nitrogenase from Rhodospirillum rubrum. Relation between 'switch-off' effect and the membrane component. Hydrogen production and acetylene reduction with different nitrogenase component ratios. Biochim Biophys Acta. 1979 Sep 11;547(3):429–437. [PubMed]
  • Nordlund S, Ludden PW. Incorporation of adenine into the modifying group of inactive iron protein of nitrogenase from Rhodospirillum rubrum. Biochem J. 1983 Mar 1;209(3):881–884. [PMC free article] [PubMed]
  • ORMEROD JG, ORMEROD KS, GEST H. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961 Sep;94:449–463. [PubMed]
  • Schick HJ. Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum. Arch Mikrobiol. 1971;75(2):89–101. [PubMed]
  • Schultz JE, Weaver PF. Fermentation and anaerobic respiration by Rhodospirillum rubrum and Rhodopseudomonas capsulata. J Bacteriol. 1982 Jan;149(1):181–190. [PMC free article] [PubMed]
  • Yoch DC. Manganese, an essential trace element for N2 fixation by Rhodospirillum rubrum and Rhodopseudomonas capsulata: role in nitrogenase regulation. J Bacteriol. 1979 Dec;140(3):987–995. [PMC free article] [PubMed]
  • Zumft WG, Castillo F. Regulatory properties of the nitrogenase from Rhodopseudomonas palustris. Arch Microbiol. 1978 Apr 27;117(1):53–60. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...