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J Bacteriol. Apr 1984; 158(1): 362–364.
PMCID: PMC215426

Genetic screen for cloned release factor genes.

Abstract

Nonsense suppression reflects competition between a nonsense suppressor tRNA and a translational release factor. This provides a simple way to screen for release factor genes cloned into a multicopy plasmid. We have confirmed that the expected competition occurs with the gene for release factor 2, cloned by C.T. Caskey et al. (C.T. Caskey, W.C. Forrester, W. Tate, and C.D. Ward, J. Bacteriol. 158:365-368, 1984), and used it to clone the gene for release factor 1.

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Selected References

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  • Beaudet AL, Caskey CT. Release factor translation of RNA phage terminator codons. Nature. 1970 Jul 4;227(5253):38–40. [PubMed]
  • Capecchi MR, Klein HA. Characterization of three proteins involved in polypeptide chain termination. Cold Spring Harb Symp Quant Biol. 1969;34:469–477. [PubMed]
  • Caskey T, Scolnick E, Tompkins R, Goldstein J, Milman G. Peptide chain termination, codon, protein factor, and ribosomal requirements. Cold Spring Harb Symp Quant Biol. 1969;34:479–488. [PubMed]
  • Caskey CT, Forrester WC, Tate W, Ward CD. Cloning of the Escherichia coli release factor 2 gene. J Bacteriol. 1984 Apr;158(1):365–368. [PMC free article] [PubMed]
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  • HORWITZ JP, CHUA J, CURBY RJ, TOMSON AJ, DAROOGE MA, FISHER BE, MAURICIO J, KLUNDT I. SUBSTRATES FOR CYTOCHEMICAL DEMONSTRATION OF ENZYME ACTIVITY. I. SOME SUBSTITUTED 3-INDOLYL-BETA-D-GLYCOPYRANOSIDES. J Med Chem. 1964 Jul;7:574–575. [PubMed]
  • Sancar A, Hack AM, Rupp WD. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. [PMC free article] [PubMed]
  • Scolnick E, Tompkins R, Caskey T, Nirenberg M. Release factors differing in specificity for terminator codons. Proc Natl Acad Sci U S A. 1968 Oct;61(2):768–774. [PMC free article] [PubMed]
  • Struhl K, Stinchcomb DT, Scherer S, Davis RW. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035–1039. [PMC free article] [PubMed]

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