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J Bacteriol. 1984 January; 157(1): 202–210. | PMCID: PMC215153 |
Genetic analysis of a streptomycin-resistant oligosporogenous Bacillus subtilis mutant. T M Henkin and G H Chambliss Abstract Strain SRB15T+, a streptomycin-resistant, oligosporogenous mutant of Bacillus subtilis, contains two mutations, fun and strR. These mutations were mapped by PBS-1 mediated transduction and by transformation to two different sites in the cysA-linked region of the B. subtilis chromosome. The fun mutation mapped very close to rpsLl, a classic strA mutation, whereas strR mapped to a site distal to rpsE. The effects of these mutations on growth, sporulation, and streptomycin resistance in vivo and in vitro were determined. The fun mutation gave a different phenotype than did the rpsLl mutation and caused altered migration of a ribosomal protein which was identified as S12, the protein encoded by rpsL. It therefore appears that fun is an allele of the rpsL gene. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Adoutte-Panvier A, Davies JE, Gritz LR, Littlewood BS. Studies of ribosomal proteins of yeast species and their hybrids: gel electrophoresis and immunochemical cross-reactions. Mol Gen Genet. 1980;179(2):273–282. [PubMed]
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