• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Oct 1983; 156(1): 81–88.
PMCID: PMC215053

2-Oxoacid dehydrogenase complexes of Escherichia coli: cellular amounts and patterns of synthesis.

Abstract

The oxidative decarboxylations of pyruvate and 2-oxoglutarate in Escherichia coli are carried out by two large, multienzyme complexes: pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase. The enzyme complexes each contain three subunits: two are unique to the individual complexes, the third is shared between them. Resolution of the polypeptide subunits on two-dimensional gels allowed quantitative analysis of their cellular levels and patterns of synthesis in growing cells. Cells growing in glucose-salts medium were found to contain roughly 85 to 136 pyruvate dehydrogenase complexes and 73 2-oxoglutarate complexes. Lipoamide dehydrogenase, the subunit shared by the two complexes, was found to be in significant excess of its stoichiometric demand in the two enzyme complexes under most growth conditions. The subunits unique to each of the complexes were coordinately regulated over a wide variety of growth conditions and a broad range of expression. The two complexes responded to different, but partially overlapping, regulatory signals. Most importantly, the shared subunit was actively regulated to accommodate its demand in both enzymes. These results are discussed with regard to possible mechanisms of regulation of the enzyme complexes in general and of the shared subunit specifically.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Amarasingham CR, Davis BD. Regulation of alpha-ketoglutarate dehydrogenase formation in Escherichia coli. J Biol Chem. 1965 Sep;240(9):3664–3668. [PubMed]
  • Bleile DM, Munk P, Oliver RM, Reed LJ. Subunit structure of dihydrolipoyl transacetylase component of pyruvate dehydrogenase complex from Escherichia coli. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4385–4389. [PMC free article] [PubMed]
  • Bloch PL, Phillips TA, Neidhardt FC. Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins. J Bacteriol. 1980 Mar;141(3):1409–1420. [PMC free article] [PubMed]
  • Blumenthal RM, Reeh S, Pedersen S. Regulation of transcription factor rho and the alpha subunit of RNA polymerase in Escherichia coli B/r. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2285–2288. [PMC free article] [PubMed]
  • Casadaban MJ, Cohen SN. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. [PMC free article] [PubMed]
  • Creaghan IT, Guest JR. Amber mutants of the -ketoglutarate dehydrogenase gene of Escherichia coli K12. J Gen Microbiol. 1972 Jul;71(2):207–220. [PubMed]
  • Derosier DJ, Oliver RM, Reed LJ. Crystallization and preliminary structural analysis of dihydrolipoyl transsuccinylase, the core of the 2-oxoglutarate dehydrogenase complex. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1135–1137. [PMC free article] [PubMed]
  • Dietrich J, Henning U. Regulation of pyruvate dehydrogenase complex synthesis in Escherichia coli K 12. Identification of the inducing metabolite. Eur J Biochem. 1970 Jun;14(2):258–269. [PubMed]
  • Gray CT, Wimpenny JW, Mossman MR. Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli. Biochim Biophys Acta. 1966 Mar 28;117(1):33–41. [PubMed]
  • Guest JR. Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: Chromosomal location of the lipoamide dehydrogenase gene. J Gen Microbiol. 1974 Feb;80(2):523–532. [PubMed]
  • Guest JR, Cole ST, Jeyaseelan K. Organization and expression of the pyruvate dehydrogenase complex genes of Escherichia coli K12. J Gen Microbiol. 1981 Nov;127(1):65–79. [PubMed]
  • Guest JR, Creaghan IT. Gene-protein relationships of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and characterization of lipoamide dehydrogenase mutants. J Gen Microbiol. 1973 Mar;75(1):197–210. [PubMed]
  • Langley D, Guest JR. Biochemical genetics of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: genetic characterization and regulatory properties of deletion mutants. J Gen Microbiol. 1978 May;106(1):103–117. [PubMed]
  • Guest JR, Stephens PE. Molecular cloning of the pyruvate dehydrogenase complex genes of Escherichia coli. J Gen Microbiol. 1980 Dec;121(2):277–292. [PubMed]
  • Hirshfield IN, Bloch PL, Van Bogelen RA, Neidhardt FC. Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli. J Bacteriol. 1981 Apr;146(1):345–351. [PMC free article] [PubMed]
  • Neidhardt FC, Bloch PL, Smith DF. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. [PMC free article] [PubMed]
  • O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed]
  • Pedersen S, Bloch PL, Reeh S, Neidhardt FC. Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell. 1978 May;14(1):179–190. [PubMed]
  • Phillips TA, Bloch PL, Neidhardt FC. Protein identifications on O'Farrell two-dimensional gels: locations of 55 additional Escherichia coli proteins. J Bacteriol. 1980 Dec;144(3):1024–1033. [PMC free article] [PubMed]
  • Smith MW, Neidhardt FC. Proteins induced by anaerobiosis in Escherichia coli. J Bacteriol. 1983 Apr;154(1):336–343. [PMC free article] [PubMed]
  • Smith MW, Neidhardt FC. Proteins induced by aerobiosis in Escherichia coli. J Bacteriol. 1983 Apr;154(1):344–350. [PMC free article] [PubMed]
  • Spencer ME, Guest JR. Molecular cloning of four tricarboxylic acid cyclic genes of Escherichia coli. J Bacteriol. 1982 Aug;151(2):542–552. [PMC free article] [PubMed]
  • Wanner BL, Kodaira R, Neidhardt FC. Physiological regulation of a decontrolled lac operon. J Bacteriol. 1977 Apr;130(1):212–222. [PMC free article] [PubMed]
  • Wanner BL, McSharry R. Phosphate-controlled gene expression in Escherichia coli K12 using Mudl-directed lacZ fusions. J Mol Biol. 1982 Jul 5;158(3):347–363. [PubMed]
  • Yamamoto I, Ishimoto M. Effect of nitrate reduction on the enzyme levels in carbon metabolism in Escherichia coli. J Biochem. 1975 Aug;78(2):307–315. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...