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J Bacteriol. Mar 1985; 161(3): 904–908.
PMCID: PMC214982

Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli.

Abstract

The nucleotide sequence of a 2220-base-pair fragment containing the btuB gene of Escherichia coli was determined. There was a single open reading frame which was translated into a 614-amino-acid polypeptide, the first 20 amino acids of which comprised a typical leader sequence. The putative mature or processed form had a molecular weight (66,400) and a composition in close agreement with that determined for the purified receptor. The distribution of amino acids in the receptor protein was similar to that of other outer membrane proteins, showing a fairly even distribution of charged residues and the absence of extensive hydrophobic stretches. The btuB451 mutation appears to alter the receptor to eliminate its ability to function in vitamin B12 uptake without affecting its ligand binding properties. The sequence of the DNA from this mutant was determined and revealed a leucine-to-proline (C-to-T transition) change in the eighth amino acid of the mature form.

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Selected References

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  • Bassford PJ, Jr, kadner RJ. Genetic analysis of components involved in vitamin B12 uptake in Escherichia coli. J Bacteriol. 1977 Dec;132(3):796–805. [PMC free article] [PubMed]
  • Biggin MD, Gibson TJ, Hong GF. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. [PMC free article] [PubMed]
  • Chai T, Wu V, Foulds J. Colicin A receptor: role of two Escherichia coli outer membrane proteins (OmpF protein and btuB gene product) and lipopolysaccharide. J Bacteriol. 1982 Aug;151(2):983–988. [PMC free article] [PubMed]
  • Chou PY, Fasman GD. Empirical predictions of protein conformation. Annu Rev Biochem. 1978;47:251–276. [PubMed]
  • Clément JM, Hofnung M. Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12. Cell. 1981 Dec;27(3 Pt 2):507–514. [PubMed]
  • Di Masi DR, White JC, Schnaitman CA, Bradbeer C. Transport of vitamin B12 in Escherichia coli: common receptor sites for vitamin B12 and the E colicins on the outer membrane of the cell envelope. J Bacteriol. 1973 Aug;115(2):506–513. [PMC free article] [PubMed]
  • Eisenberg D. Three-dimensional structure of membrane and surface proteins. Annu Rev Biochem. 1984;53:595–623. [PubMed]
  • Garavito RM, Jenkins JA, Neuhaus JM, Pugsley AP, Rosenbusch JP. Structural investigations of outer membrane proteins from Escherichia coli. Ann Microbiol (Paris) 1982 Jan;133A(1):37–41. [PubMed]
  • Grosjean H, Fiers W. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene. 1982 Jun;18(3):199–209. [PubMed]
  • Heller K, Mann BJ, Kadner RJ. Cloning and expression of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli. J Bacteriol. 1985 Mar;161(3):896–903. [PMC free article] [PubMed]
  • Imajoh S, Ohno-Iwashita Y, Imahori K. The receptor for colicin E3. Isolation and some properties. J Biol Chem. 1982 Jun 10;257(11):6481–6487. [PubMed]
  • Inokuchi K, Mutoh N, Matsuyama S, Mizushima S. Primary structure of the ompF gene that codes for a major outer membrane protein of Escherichia coli K-12. Nucleic Acids Res. 1982 Nov 11;10(21):6957–6968. [PMC free article] [PubMed]
  • Kadner RJ. Repression of synthesis of the vitamin B12 receptor in Escherichia coli. J Bacteriol. 1978 Dec;136(3):1050–1057. [PMC free article] [PubMed]
  • Kadner RJ, Liggins GL. Transport of vitamin B12 in Escherichia coli: genetic studies. J Bacteriol. 1973 Aug;115(2):514–521. [PMC free article] [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Messing J, Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. [PubMed]
  • Michaelis S, Beckwith J. Mechanism of incorporation of cell envelope proteins in Escherichia coli. Annu Rev Microbiol. 1982;36:435–465. [PubMed]
  • Mizuno T, Chou MY, Inouye M. A comparative study on the genes for three porins of the Escherichia coli outer membrane. DNA sequence of the osmoregulated ompC gene. J Biol Chem. 1983 Jun 10;258(11):6932–6940. [PubMed]
  • Neilands JB. Microbial envelope proteins related to iron. Annu Rev Microbiol. 1982;36:285–309. [PubMed]
  • Nikaido H, Wu HC. Amino acid sequence homology among the major outer membrane proteins of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1048–1052. [PMC free article] [PubMed]
  • Overbeeke N, Bergmans H, van Mansfeld F, Lugtenberg B. Complete nucleotide sequence of phoE, the structural gene for the phosphate limitation inducible outer membrane pore protein of Escherichia coli K12. J Mol Biol. 1983 Feb 5;163(4):513–532. [PubMed]
  • Reynolds PR, Mottur GP, Bradbeer C. Transport of vitamin B12 in Escherichia coli. Some observations on the roles of the gene products of BtuC and TonB. J Biol Chem. 1980 May 10;255(9):4313–4319. [PubMed]
  • Rosenberg M, Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. [PubMed]
  • Sabet SF, Schnaitman CA. Purification and properties of the colicin E3 receptor of Escherichia coli. J Biol Chem. 1973 Mar 10;248(5):1797–1806. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Shine J, Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. [PMC free article] [PubMed]
  • Staden R. A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Res. 1980 Aug 25;8(16):3673–3694. [PMC free article] [PubMed]
  • Stormo GD, Schneider TD, Gold LM. Characterization of translational initiation sites in E. coli. Nucleic Acids Res. 1982 May 11;10(9):2971–2996. [PMC free article] [PubMed]

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