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Logo of bmcdbBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Developmental Biology
BMC Dev Biol. 2007; 7: 113.
Published online Oct 12, 2007. doi:  10.1186/1471-213X-7-113
PMCID: PMC2148063

Sex-specific DoublesexM expression in subsets of Drosophila somatic gonad cells

Abstract

Background

In Drosophila melanogaster, a pre-mRNA splicing hierarchy controls sexual identity and ultimately leads to sex-specific Doublesex (DSX) transcription factor isoforms. The male-specific DSXM represses genes involved in female development and activates genes involved in male development. Spatial and temporal control of dsx during embryogenesis is not well documented.

Results

Here we show that DSXM is specifically expressed in subsets of male somatic gonad cells during embryogenesis. Following testis formation, germ cells remain in contact with DSXM-expressing cells, including hub cells and premeiotic somatic cyst cells that surround germ cells during spermatogenesis in larval and adult testes.

Conclusion

We show that dsx is transcriptionally regulated in addition to being regulated at the pre-mRNA splicing level by the sex determination hierarchy. The dsx locus is spatially controlled by somatic gonad identity. The continuous expression of DSXM in cells contacting the germline suggests an ongoing short-range influence of the somatic sex determination pathway on germ cell development.

Background

A regulatory cascade directs all aspects of somatic sexual differentiation in Drosophila, including somatic gonad formation [1-3]. This hierarchy is composed of a series of alternative pre-mRNA processing regulators. Diploid flies with two X chromosomes are female (XX:AA) and those with one are male (X:AA). The Sex-lethal (SXL) protein is ubiquitously expressed in early XX:AA embryos and directs female splicing of later appearing Sxl and transformer (tra) pre-mRNA such that functional SXL and TRA proteins are produced only in females. Presence of TRA and the constitutive product of transformer-2 (tra2) in females lead to female-specific splicing of the doublesex (dsx) pre-mRNA, which then gives rise to DSXF protein. In X:AA flies, the absence of SXL, and thus TRA, results in male-specific splicing of dsx pre-mRNA. Male-specific dsx mRNA encodes DSXM protein. Both DSXF and DSXM are zinc-finger transcription factors of the DMRT family. Members of this family play important roles in sex determination in most animals that have been examined to date [4].

The Drosophila DSX proteins possess identical N-terminal DNA-binding domains but differ in their C-termini [5-7]. DSXM is thought to repress genes that are involved in female development and activate male differentiation genes while DSXF is thought to do the opposite [8-11]. Although there are a few aspects of sexual dimorphism that are not controlled by DSX, a plethora of phenotypes including elaboration of the abdominal pigmentation, development of the genitalia, sex combs and abdominal neuroblasts, as well as certain aspects of male courtship behavior depend on this important regulator of sexual dimorphism [8,12,13].

The dsx locus plays a critical role in both somatic gonad development [14] and specification of germline sexual identity [11,15]. Flies transformed from females to males by constitutive expression of DSXM have testes but very few germ cells. These germ cells can show evidence of either male or female development. However, dsx is not required within the germline cells, suggesting that the role of dsx in germline development is non-autonomous [16]. Thus, DSX expression is expected in somatic cells that communicate with the germline. Developmental northern blots have shown that there are multiple dsx transcripts in larvae and adults [9]. Despite the importance of dsx in both somatic gonad and germline development, very little is known about when and where DSX is expressed during gonadogenesis.

Gonad development in Drosophila is initiated in the embryo [17]. Germ cells form at the posterior pole of the embryo, divide, are carried into the embryo during gastrulation, migrate through the future gut, and coalesce. Recent work has shown that mesodermal cells from the abdominal region, as well as the germline cells, undergo a well-defined set of migrations to the presumptive gonad and then coalesce into the gonad [18-20]. Additional somatic cells, which express SOX100B, are recruited and maintained in the embryonic testis but not in the ovary; these cells are called male-specific somatic gonadal precursors [21]. Following gonad formation, male germline divisions, regulated by the JAK/STAT pathway, begin [22], whereas there are no divisions of female germ cells at this stage.

In this report we show that the male-specific isoform of dsx mRNA is expressed in the embryo. We have developed an antibody that detected the DSXM isoform and show that, in contrast to SXL, which is expressed uniformly throughout the embryo [23,24], DSXM expression was restricted to the initial somatic cells that form the somatic gonad in male embryos. Furthermore, DSXM is expressed in the male-specific somatic gonadal precursors that are later recruited to the gonad and are maintained in male embryos. DSXM is not detected outside the gonad, in the germ cells, or in the late arriving somatic cells that surround the embryonic testis. Male germ cells are in direct contact with DSXM-expressing somatic cells through adulthood, as DSXM is specifically expressed in the two somatic cyst cells surrounding developing germline cysts during pre-meiotic spermatogenesis. Only differentiating sperm appear to be unaccompanied by DSXM-positive somatic cells.

Results

dsxm transcripts in male embryos

A systematic survey of embryonic expression patterns has shown that dsx transcripts are not maternally deposited and are specifically expressed in the somatic precursors of the gonads just prior to gonad coalescence [25]. However, this global survey did not determine the sex of the embryos or specify the dsx mRNA isoform. We sorted male and female embryos bearing a female-specific Sxl early promoter [24] attached to eGFP and performed RT-PCR experiments using mRNA from sexed 3–10 hour, 10–16 hour and 16–22 hour old embryos (Fig. 1A–C) to determine if dsxm mRNA is expressed in embryos. dsxm mRNA was not readily detected in 3–10 hour old embryos of either sex, but was easily detected in 10–16 hour and 16–22 hour old male embryos. Sequencing of the RT-PCR products verified that they derived from predicted dsxm transcripts. Thus, RT-PCR experiments showed that dsxmis expressed solely in male embryos.

Figure 1
dsxm and DSXM in embryos. (A) RT-PCR of dsxm from sex sorted embryos of ages 3–10 hours; 10–16 hours and 16–22 hours. (B) β3-tubulin amplification control. (C) Cartoon of dsx transcripts. The positions of the primers used ...

DSXM expression in the male embryonic gonad

To further dissect the DSXM expression pattern, we raised polyclonal antisera against a peptide from the male-specific C-terminus of DSXM. To determine antibody specificity we performed immuno-labeling experiments focusing on whether: 1) the cell-staining pattern matched the in situ hybridization pattern, 2) the signal was male-specific and 3) the signal was absent from male embryos mutant for dsx.

The anti-VASA antibody was used to detect germ cells, which served as a guide to follow somatic cells of the gonad. In wild-type male embryos, cells intermingled with VASA-positive germ cells were clearly stained with anti-DSXM during embryonic stage 13 (Fig (Fig1F).1F). Based on position, these were the somatic gonad precursor cells. The DSXM staining was nuclear based on coincident DAPI staining for DNA (additional file 1). Additionally, the pattern of anti-DSXM immunofluorescence coincided with the dsxm transcription pattern detected by in situ hybridization previously [25], and in this study (not shown). Finally, we did not observe anti-DSXM immunofluorescence in either female embryos (Fig 1D, E), or in embryos expressing a dsx mRNA truncated upstream of the region encoding the epitope used for antibody generation (genotype = Df(3R)dsx15/In(3R)dsx23) (Fig 1H, I).

To better determine the identity of DSXM-expressing cells, we performed co-immunofluorescence staining experiments with anti-DSXM and antibodies against several somatic gonad precursor markers. We focused on mid- to late embryogenesis when DSXM is strongly expressed and when gonad formation occurs (Fig (Fig22).

Figure 2
Gonad development. Foregut and hindgut (dark-gray); anterior and posterior midgut (light-gray); somatic gonadal precursors (purple); germ cells (yellow); male-specific somatic gonadal precursors (orange); somatic gonadal precursors of the hub (red); and ...

We first ascertained the specific cell type/s that express DSXM by using anti-VASA to detect germ cells and an antibody against the transcription factor Traffic jam (TJ) to detect the somatic gonad precursors [26]. The double labeling with anti-VASA and anti-DSXM indicated that DSXM was not expressed in the germline. Anti-TJ and anti-DSXM staining indicated that DSXM was expressed in somatic cells intermingled with the germline in stage 13 embryos (Fig. 3A–H), stage 15 embryos (Fig. 3I–P), as well as in later embryonic stages.

Figure 3
DSXM and TJ expression in the male somatic gonad. (A-H) Stage 13 male embryo immunofluorescence using: (A) anti-DSXM, (B) anti-TJ, and (C) anti-VASA. (D) Merged images A-C. (E-H) Magnified view of the gonad in A-D. Somatic nuclei expressing DSXM but not ...

The overlap in TJ and DSXM staining was consistent with DSXM expression in all somatic gonad precursors in the embryonic testis. However, at the very posterior of the coalescing gonad we detected DSXM expression in cells that did not show TJ staining (Fig. 3E, H and 3M, P arrows), but were clearly assembled into the embryonic testis. TJ is reported to be expressed in all somatic gonad cell precursors [26], but our data suggest that TJ is not expressed in the posterior somatic precursors. Based on position and the confirming experiments outlined below, these DSXM-positive and TJ-negative cells were the male-specific somatic gonad cells. This indicates that TJ is not expressed in all somatic gonad cells.

To further investigate DSXM expression in the somatic gonad we used anti-Eyes absent (EYA). EYA is a transcription factor, which is also expressed in the somatic gonad [27]. EYA was found in the entire somatic gonad including the cluster of posterior TJ-negative somatic gonad cells. Staining for EYA and DSXM revealed co-expression at the cellular level throughout the entire somatic gonad of stage 13 and older embryos (Fig. 4A–D). These data suggest that a small population of somatic gonad cells expresses DSXM and EYA, but not TJ.

Figure 4
DSXM but not TJ is expressed in male-specific somatic gonadal precursors. (A-D) Stage 13 testis immunofluorescence using (A) anti-DSXM, (B) anti-EYA, and (C) anti-VASA. (D) Merged images A-C. A DSXM and EYA positive cluster of cell nuclei is located posterior ...

To ascertain the identity of the posterior most cells of the somatic gonad more directly, we applied anti-DSXM in combination with anti-SOX100B, because the male-specific somatic gonad precursor cells are reported to be the only gonad cells expressing SOX100B [28]. Anti-DSXM and anti-SOX100B co-immunofluorescence revealed DSXM expression in male-specific somatic gonad precursors (Fig. 4E–L). Absence of TJ in a subset of SOX100B-expressing cells was confirmed by counter-staining with anti-SOX100B (Fig. 4M–P). There were clearly cells expressing SOX100B, but not TJ. A few cells appeared to express both SOX100B and TJ. Taken together, these data indicate that DSXM is expressed in all somatic gonad cells expressing EYA and either TJ or SOX100B.

Interestingly, SOX100B was also expressed in another population of cells that wrapped around the gonad in stage 17 embryos (Fig. 5A–D). These might have been the precursors of the testis sheath [17], but we did not investigate the fate or function of these cells in this study. We did not observe DSXM expression in these SOX100B-positive cells surrounding the embryonic testis (Fig. 5A–D).

Figure 5
DSXM is not expressed in all somatic testis cells. (A-D) stage 17 testis immunofluorescence using (A) anti-DSXM, (B) anti-SOX100B, and (C) anti-VASA. (D) Merged images A-C. (E-H) stage 17 testis immunofluorescence (imaged in a focal plane with the hub) ...

The testis hub is part of the niche and required to maintain male germline stem cell identity. The somatic hub cells of the male gonad can be identified in stage 17 embryonic testes as an anterior cluster of somatic cells expressing the cell adhesion molecule Fasciclin III (FAS III) [29]. In anti-DSXM and anti-FAS III co-immunofluorescence experiments, we observed strong DSXM expression in hub cells that were outlined by anti-FAS III labeling (Fig. 5E–H). Taken together, our data indicate that DSXM is expressed in all the somatic cells of the embryonic testis identified with somatic gonad markers, except the SOX100B-positive cells that surround the embryonic testis at stage 17.

DSXM is expressed during spermatogenesis in cyst cells prior to meiosis

In contrast to many other cell types, the germline stem cells continue to divide in adults in order to produce gametes. If germline sexual identity is irreversibly determined during embryogenesis, there may be no need for post-embryonic somatic DSXM expression. However, if sex determination or maintenance of sexual identity is an ongoing process, then germline sexual identity might require continued expression of DSX. We readily detected dsxm transcripts by RT-PCR with RNA from adult testes (not shown). To explore the cellular expression pattern of DSXM in testes of larvae and adult flies, we performed whole-mount antibody immunofluorescence staining experiments.

Male germline stem cells in close contact with the hub undergo asymmetric divisions to regenerate the stem cell population and produce the cells that develop into sperm [30]. Cells remaining at the hub are the stem cells. We observed DSXM expression in the somatic hub cells of larval (Fig. 6A–D) and adult testes (Fig. 6E–H), where it also co-localized with TJ. These data suggest that male germline stem cells are always in contact with DSXM-expressing cells.

Figure 6
DSXM in hub cells. (A-D) Larval (3rd instar) testis immunofluorescence using (A) anti-DSXM, (B) anti-TJ, and (C) anti-FAS III. (D) Merged images A-C. (E-F) Adult testis immunofluorescence using (E) anti-DSXM, (F) anti-TJ, and (G) anti-FAS III. (H) Merged ...

Germline cells leaving the niche divide four times to produce 16-cell cysts. Germline cysts are enveloped by two somatic cyst cells, which become flattened as the germline cysts enlarge 20-fold in volume in preparation for meiosis. These engorged cells are highly transcriptionally active. Sperm differentiation occurs postmeiotically under translational control [30]. The transcription factor TJ is expressed in the cyst progenitor cells at the apex and in early cyst cells enveloping the dividing germ cells and then fades in later cysts [26]. EYA expression becomes stronger during this progression, such that the two patterns are partially complementary [31]. Co-immunofluorescence with anti-DSXM, anti-TJ and anti-EYA revealed that there was overlapping expression of the three proteins in these somatic cyst cells (Fig. (Fig.7).7). Anti-DSXM staining of larval and adult testes revealed expression in early cyst cells as evidenced by position within the gonad and the overlap with TJ. When TJ expression faded, DSXM expression persisted in late cyst cells and co-localized with EYA (Fig. 7A–D and 7E–H). Interestingly, anti-DSXM staining was never observed in cyst cells surrounding transcriptionally quiescent post-meiotic stages (not shown). These data are consistent with the idea that the determination or maintenance of germ cell sexual identity requires ongoing DSXM expression until the end of spermatogenic transcription at meiosis, but is no required once germline transcription ceases.

Figure 7
DSXM expression during spermatogenesis. (A-D) Larval testis immunofluorescence using (A) anti-DSXM, (B) anti-TJ, and (C) anti-EYA. (D) Merged images A-C. (E-H) Higher magnification view of A-D. (I-L) Adult apical testis immunofluorescence using (I) anti-DSX ...

DSXM expression does not require a germline, EYA, or TJ

Testis formation depends on the collaboration of germ cells and distinct somatic cell types. However, somatic gonad formation is independent of germ cells [17,32]. Therefore, we predicted that functional DSXM expression should not be dependent on a germline. To investigate whether germ cells were necessary for DSXM expression in somatic gonadal precursor cells, we examined embryos lacking germ cells due to the maternal grandchildless mutation gs(l)N26 [33]. Unsurprisingly, anti-DSXM staining was readily detectable in germlineless embryonic testes (Fig. 8A–D) indicating that the germline is not required to induce or maintain DSXM expression.

Figure 8
DSXM expression does not require germ cells nor EYA or TJ. (A-D) Agametic testis of stage 15 males from gs(1)N26 mothers. Immunofluorescence using (A) anti-DSXM, (B) anti-TJ, and (C) anti-VASA. (D) Merged images A-C. (E-H) Isolated somatic gonadal precursors ...

The eya gene was a candidate regulator of dsx expression as EYA was always expressed in DSXM-positive gonad cells, and preceded DSXM expression temporally in the embryo (EYA was strongly expressed in somatic gonadal precursor cells of stage 12 embryos, when we did not readily detect DSXM expression). We therefore determined whether DSXM expression required EYA function. This experiment was complicated by the fact that EYA is essential for gonadogenesis. In late embryonic stages, eya mutant embryos have germ cells that are scattered throughout posterior regions and only a few somatic gonad precursor cells develop. The few somatic gonad precursors do not coalesce into a gonad, rather they form clumps with associated germ cells [27]. In homozygous eya mutant male embryos, we were able to detect DSXM expression in the sporadically formed somatic gonad precursor cells (Fig. 8E–H). These sporadically formed cells also expressed TJ. Expression of DSXM and TJ in these rarely formed somatic gonad precursor cells suggests that eya is not obligatory for expression of TJ or DSXM in the embryonic testis. However, it should be noted that there are very few TJ- and DSXM-positive cells in eya mutants. Those somatic gonad cells that escape may not be representative.

TJ was expressed in many DSXM-positive somatic cells and was therefore a potential regulator of dsx. We examined tjeo2 male embryos to determine if DSXM expression depends upon TJ. The tjeo2 allele [26] encodes a truncated protein lacking the two DNA-binding domains, a putative bipartite nuclear localization signal, and the leucine-zipper domain. The allele behaves as a genetic null. In gonads of tj mutant embryos, germline divisions during late embryogenesis are hindered. Additionally, somatic gonad cells do not intermingle with germ cells and remain at the gonad periphery [26]. In homozygous tj mutant male embryos, we detected DSXM expression in all the somatic gonad cells (Fig. 8I–L). Thus, DSXM expression in somatic gonad cells is not dependent upon the activity of TJ.

Discussion

Spatial and sexual regulation of DSXM

The pre-mRNA splicing cascade regulating somatic sexual development has been well studied (Fig (Fig9A).9A). The X-chromosome number is read by the uniform expression of X chromosome transcription factors, which uniformly and transiently activate Sxl expression in XX:AA flies prior to general activation of the zygotic genome [3]. This transient expression of the SXL splicing factor results in an autoregulatory loop when Sxl pre-mRNA is produced from a ubiquitous promoter active later in development. In X:AA flies, SXL is not present and dosage compensation complexes form in all X:AA cells to increase X chromosome gene expression [34]. Addressing chromosome dose imbalance should be important for all cells. The uniform and early expression of SXL to prevent over-expression of X chromosome genes, via inhibition of male-specific-lethal-2 (MSL2) in females, is therefore quite logical. The absence of SXL also ultimately results in DSXM production. As many aspects of sexual dimorphism in the soma require DSX function [8,14,35-38], there is no a priori requirement for tissue-specific expression of dsx.

Figure 9
DSXM regulation. DSXM is regulated by the intersection of the sex-determination alternative pre-mRNA splicing hierarchy and spatial/temporal regulation (A). Positive (green arrows) and negative interactions (red) are indicated. See text for details. Cellular ...

Here we show that DSXM expression in male embryos is restricted to somatic gonadal cells that form several hours after SXL expression is initiated. Consequently, in addition to the sex determination hierarchy, it is likely that DSXM expression depends on additional and yet unknown activators expressed in embryonic somatic gonadal precursor cells or repressors in non-gonadal somatic cells. It is also likely that DSXF is expressed in the corresponding somatic gonad in females, but we have not been able to generate useful antibodies against the predicted female isoform, or the region common to DSXM and DSXF. Expression of dsx in subsets of the nervous system [39] raises the possibility that transcriptional deployment of dsx pre-mRNAs in female or male pre-mRNA splicing environments is a common theme.

Defining subsets of somatic testis cells

Two main cell types compose the embryonic Drosophila gonad: germ cells and somatic gonad cells [19]. Our results suggest that each somatic gonadal cell of a male stage 13 embryo has a male identity before overt morphological testis development is evident. By stage 17, embryonic gonads are clearly sexually dimorphic (Fig (Fig9B).9B). The hub, a cluster of specialized somatic cells required for germline stem cell maintenance, forms anteriorly in stage 17 testes [29]; these hub cells express DSXM. We have also seen a monolayer of cells around the testis during stage 17, possibly the testis sheath precursors. These late additions to the embryonic testis do not express DSXM but do express SOX100B.

Somatic DSXM and the germline

Although DSXM is required to promote male development and to repress female development in several somatic tissues, the role of DSXM activity in embryonic testis morphogenesis is unclear as dsx mutants are strikingly similar to wildtype males [21]. However, it is clear that DSXM regulates the expression of the STAT92E transcription factor in male embryonic germ cells [22]. Additionally, DSXM results in male-specific splicing of Sxl pre-mRNA in adult germ cells [15]. Perhaps the major role of somatic DSXM in the embryo is regulation of the germline gene expression program. The non-autonomous role of DSXM is not restricted to germline development, as DSXM also non-autonomously regulates the post-embryonic recruitment of mesodermal cells to the male genital disc [40].

DSXM-expressing cells are in intimate contact with the germline, which may be important to enable the somatic sex determination pathway to influence germ cell development in the embryonic gonad. Somatic gonadal precursors undergo striking shape changes as the gonad coalesces, producing thin cellular extensions that surround the round germ cells at stage 13 [41]. DSXM expression becomes apparent at this stage.

There is also contact between germline and DSXM-positive somatic cells in post-embryonic stages. We found that DSXM is expressed in testes of larvae and adult flies in the somatic cyst cells enveloping all premeiotic germ cell cysts as well as in the hub. DSXM expression was never observed in cyst cells enclosing postmeiotic germ cells, which have completed the transcriptional program required for sperm differentiation [42,43]. Thus, DSXM appears to be expressed in all the somatic cells that are closely associated with the germ cells from gonad formation until the transcriptional program of spermatogenesis is complete.

Methods

Flies

All flies were raised and maintained on standard cornmeal media (Tucson Drosophila stock center, Tucson, AZ) at 25°C. A fly strain bearing a construct containing the early Sex lethal promoter, SxlPE, [24] upstream of the coding sequence for eGFP on chromosome 3 (Sxl-GFP-3) was used for sexing embryos [44]. Only female embryos of this line express eGFP. Dechorionated embryos were sex sorted by distinguishing fluorescent versus non-fluorescent embryos using the Copas Select sorter (UNION Biometrica, Holliston, MA). 60 embryos from each collection were set aside and allowed to mature to score the sexing efficiency. Only collections showing 100% sexing fidelity were used in experiments. The following mutant alleles were used for analyses: gs(l)N26 [45]; Df(3R)dsx15 and In(3R)dsx23 [46]; eyacli-IIEand eyacli-DI [47] and tjeo2 [26].

RT-PCR

Total RNA was prepared using TRIzol reagent (Invitrogen, Carlsbad, CA) from 3–10, 10–16, 16–22 hour old, sex sorted embryos as well as from testes of male adult flies. Preparation of polyA+-mRNA from all samples was done using the Oligotex mRNA Mini Kit (Qiagen, Valencia, CA). RT-PCR was performed using the OneStep RT-PCR Kit (Qiagen, Valencia, CA). A pair of primers (RT-sense 5'-CGCGCACCACGTCCACATGGCAGCTG-3'; male-antisense 5'-CTCTGGAGTCGGTGGACAAATCTGTGTG-3') flanking two introns was used to amplify an 801 bp cDNA fragment from the dsxm transcript. The amplicons were sequenced for verification. For loading control a separate RT-PCR of each mRNA sample was carried out in parallel using a primer pair (β3 sense 5'-ATCATTTCCGAGGAGCACGGC-3'; β3 antisense 5'-GCCCAGCGAGTGCGTCAATTG-3') for the ubiquitously expressed gene β3-tubulin [48], which amplifies a 397 bp fragment from the transcript.

Antibodies and immunofluorescence staining

The polyclonal DSXM antibody was made using standard methods (Covance, Princeton, NJ). A peptide containing the C-terminal amino acids SSNGAYHHGHHL was used for immunization of two rats, both of which gave useful antibodies of good titer and were used here.

For cell staining experiments, pre-stage 17 embryos were fixed, devitellinized, and immunostained as described [49]. Because the presence of the cuticle greatly hinders antibody staining, we used a different protocol for stage 17. Fixed stage 17 embryos were rehydrated, washed twice in 1 × PBS + 0.1% Tween 20 (PBT), sonicated in 500 μl PBT (2 pulses of 3 sec. using a Misonix Sonicator 3000 at output setting 1), rewashed twice in PBT and immunostained [49]. Gonads of larvae and adult flies were dissected, fixed and immunostained as described [50]. All stained samples were mounted on slides in Fluoromount-G (Southern Biotech, Birmingham, AL).

Antibodies were as follows: mouse anti-FASIII and mouse anti-EYA10H6 (Developmental Studies Hybridoma Bank, Iowa City, IA) at 1:50, rabbit anti-SOX100B [28] at 1:1000, guinea pig anti-TJ [26] at 1:3000, rabbit anti-VASA (R. Lehmann) at 1:5000, chicken anti-VASA [51] at 1:1000. For the rat anti-DSXM at 1:500 in general and the rabbit anti-SOX100B at 1:1000 (in Fig Fig4N)4N) the TSA (Tyramide Signal Amplification) Cyanine 3 technology (Perkin Elmer, Waltham, MA) in combination with the ABC Kit (Vector Laboratories, Burlingham, CA) was used as the detection system.

The following secondary antibodies were used, all at 1:500: Cy5 goat anti-rabbit, biotin-coupled goat anti-rat, and biotin-coupled goat anti-rabbit (Jackson Immuo-research, West Grove, PA), Alexa 647 goat anti-guinea pig, Alexa 488 goat anti-guinea pig, Alexa 488 goat anti-rabbit, Alexa 647 goat anti-mouse, Alexa 488 goat anti-mouse, Alexa 647 goat anti-chicken (Invitrogen Molecular Probes, Carlsbad, CA). Images were acquired using a Zeiss LSM 510 Meta Confocal Microscope (Zeiss, Thornwood, NY) and processed using Photoshop 7.0 (Adobe, San Jose, CA). All figures were annotated using Illustrator 10.0 (Adobe, San Jose, CA). All images in figures are single optical sections as indicated.

DAPI (4',6-diamidino-2-phenylindole, dihydrochloride) staining was used to visualize the nuclei.

Supplementary Material

Additional file 1:

DSXM localizes to the nucleus. (A-D) Stage 13 testis immunofluorescence using (A) anti- DSXM, (B) anti-VASA, and (C) DAPI. (D) Merged images A-C. (E-H) Stage 15 male testis immunofluorescence using (E) anti- DSXM, (F) anti-VASA and (G) DAPI. (H) Merged images E-G. The scale bars = 10 mm. Anterior is to the left.

Acknowledgements

We thank S. DiNardo, M. van Doren, D. Godt, P. Graham, K. Howard, S. Kobayashi, R. Lehmann, S. Russell, P. Schedl, the Developmental Studies Hybridoma Bank and the Bloomington Stock Center for flies and reagents. We are grateful to members of the Oliver and van Doren labs as well as members of the LCDB for discussion. We also thank the anonymous referees for valuable suggestion on the manuscript. This research was supported by the Intramural Research Program of the NIH, NIDDK.

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