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J Bacteriol. Apr 1986; 166(1): 338–340.
PMCID: PMC214599

Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.

Abstract

Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportional to the DNA concentration from 10 pg/ml to 100 ng/ml. T. thermophilus HB27 cells did not require chemical treatment to induce competence, although optimal transformation was obtained by the addition of a divalent cation (Ca2+ or Mg2+). Competence was maintained throughout the growth phase, with the highest transformation frequencies at pH 6 to 9 and at 70 degrees C. T. thermophilus HB27 and four other typical Thermus strains, T. thermophilus HB8, T. flavus AT62, T. caldophilus GK24, and T. aquaticus YT1, were also transformed to streptomycin resistance by DNA from their own spontaneous streptomycin-resistant mutants. A cryptic plasmid, pTT8, from T. thermophilus HB8 was introduced into T. thermophilus HB27 Pro- at a frequency of 10(-2).

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Selected References

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  • Brock TD, Freeze H. Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J Bacteriol. 1969 Apr;98(1):289–297. [PMC free article] [PubMed]
  • Freeze H, Brock TD. Thermostable aldolase from Thermus aquaticus. J Bacteriol. 1970 Feb;101(2):541–550. [PMC free article] [PubMed]
  • Fujita SC, Oshima T, Imahori K. Purification and properties of D-glyceraldehyde-3-phosphate dehydrogenase from an extreme thermophile, Thermus thermophilus strain HB 8. Eur J Biochem. 1976 Apr 15;64(1):57–68. [PubMed]
  • Grunstein M, Wallis J. Colony hybridization. Methods Enzymol. 1979;68:379–389. [PubMed]
  • Hishinuma F, Tanaka T, Sakaguchi K. Isolation of extrachromosomal deoxyribonucleic acids from extremely thermophilic bacteria. J Gen Microbiol. 1978 Feb;104(2):193–199. [PubMed]
  • Kagawa Y, Nojima H, Nukiwa N, Ishizuka M, Nakajima T, Yasuhara T, Tanaka T, Oshima T. High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus. J Biol Chem. 1984 Mar 10;259(5):2956–2960. [PubMed]
  • Nagahari K, Koshikawa T, Sakaguchi K. Cloning and expression of the leucine gene from Thermus thermophilus in Escherichia coli. Gene. 1980 Jul;10(2):137–145. [PubMed]
  • Nojima H, Oshima T, Noda H. Purification and properties of phosphoglycerate kinase from Thermus thermophilus strain HB8. J Biochem. 1979 Jun;85(6):1509–1517. [PubMed]
  • Piechowska M, Fox MS. Fate of transforming deoxyribonucleate in Bacillus subtilis. J Bacteriol. 1971 Nov;108(2):680–689. [PMC free article] [PubMed]
  • Pifer ML, Smith HO. Processing of donor DNA during Haemophilus influenzae transformation: analysis using a model plasmid system. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3731–3735. [PMC free article] [PubMed]
  • Ramaley RF, Hixson J. Isolation of a nonpigmented, thermophilic bacterium similar to Thermophilic bacterium similar to Thermus aquaticus. J Bacteriol. 1970 Aug;103(2):527–528. [PMC free article] [PubMed]
  • SAITO H, MIURA KI. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed]
  • Sakaki Y, Oshima T. Isolation and characterization of a bacteriophage infectious to an extreme thermophile, Thermus thermophilus HB8. J Virol. 1975 Jun;15(6):1449–1453. [PMC free article] [PubMed]
  • Taguchi H, Yamashita M, Matsuzawa H, Ohta T. Heat-stable and fructose 1,6-bisphosphate-activated L-lactate dehydrogenase from an extremely thermophilic bacterium. J Biochem. 1982 Apr;91(4):1343–1348. [PubMed]
  • Tanaka T, Kawano N, Oshima T. Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product. J Biochem. 1981 Feb;89(2):677–682. [PubMed]
  • Watanabe K, Oshima T, Iijima K, Yamaizumi Z, Nishimura S. Purification and thermal stability of several amino acid-specific tRNAs from an extreme thermophile, Thermus thermophilus HB8. J Biochem. 1980 Jan;87(1):1–13. [PubMed]

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