• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of prosciprotein sciencecshl presssubscriptionsetoc alertsthe protein societyjournal home
Protein Sci. Jan 1997; 6(1): 249–253.
PMCID: PMC2143507

SAM as a protein interaction domain involved in developmental regulation.


More than 60 previously undetected SAM domain-containing proteins have been identified using profile searching methods. Among these are over 40 EPH-related receptor tyrosine kinases (RPTK), Drosophila bicaudal-C, a p53 from Loligo forbesi, and diacyglycerol-kinase isoform delta. This extended dataset suggests that SAM is an evolutionary conserved protein binding domain that is involved in the regulation of numerous developmental processes among diverse eukaryotes. A conserved tyrosine in the SAM sequences of the EPH related RPTKs is likely to mediate cell-cell initiated signal transduction via the binding of SH2 containing proteins to phosphotyrosine.

Full Text

The Full Text of this article is available as a PDF (1.9M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Bardwell L, Cook JG, Inouye CJ, Thorner J. Signal propagation and regulation in the mating pheromone response pathway of the yeast Saccharomyces cerevisiae. Dev Biol. 1994 Dec;166(2):363–379. [PubMed]
  • Barr MM, Tu H, Van Aelst L, Wigler M. Identification of Ste4 as a potential regulator of Byr2 in the sexual response pathway of Schizosaccharomyces pombe. Mol Cell Biol. 1996 Oct;16(10):5597–5603. [PMC free article] [PubMed]
  • Bender L, Lo HS, Lee H, Kokojan V, Peterson V, Bender A. Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast. J Cell Biol. 1996 May;133(4):879–894. [PMC free article] [PubMed]
  • Birney E, Thompson JD, Gibson TJ. PairWise and SearchWise: finding the optimal alignment in a simultaneous comparison of a protein profile against all DNA translation frames. Nucleic Acids Res. 1996 Jul 15;24(14):2730–2739. [PMC free article] [PubMed]
  • Bork P, Gibson TJ. Applying motif and profile searches. Methods Enzymol. 1996;266:162–184. [PubMed]
  • Bornemann D, Miller E, Simon J. The Drosophila Polycomb group gene Sex comb on midleg (Scm) encodes a zinc finger protein with similarity to polyhomeotic protein. Development. 1996 May;122(5):1621–1630. [PubMed]
  • DeCamillis M, Cheng NS, Pierre D, Brock HW. The polyhomeotic gene of Drosophila encodes a chromatin protein that shares polytene chromosome-binding sites with Polycomb. Genes Dev. 1992 Feb;6(2):223–232. [PubMed]
  • van der Geer P, Hunter T, Lindberg RA. Receptor protein-tyrosine kinases and their signal transduction pathways. Annu Rev Cell Biol. 1994;10:251–337. [PubMed]
  • Gribskov M, McLachlan AD, Eisenberg D. Profile analysis: detection of distantly related proteins. Proc Natl Acad Sci U S A. 1987 Jul;84(13):4355–4358. [PMC free article] [PubMed]
  • Hirai H, Maru Y, Hagiwara K, Nishida J, Takaku F. A novel putative tyrosine kinase receptor encoded by the eph gene. Science. 1987 Dec 18;238(4834):1717–1720. [PubMed]
  • Hynes RO, Lander AD. Contact and adhesive specificities in the associations, migrations, and targeting of cells and axons. Cell. 1992 Jan 24;68(2):303–322. [PubMed]
  • Iwase T, Tanaka M, Suzuki M, Naito Y, Sugimura H, Kino I. Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer. Biochem Biophys Res Commun. 1993 Jul 30;194(2):698–705. [PubMed]
  • Jackman JK, Motto DG, Sun Q, Tanemoto M, Turck CW, Peltz GA, Koretzky GA, Findell PR. Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells. J Biol Chem. 1995 Mar 31;270(13):7029–7032. [PubMed]
  • Karin M. Signal transduction from cell surface to nucleus in development and disease. FASEB J. 1992 May;6(8):2581–2590. [PubMed]
  • Kiyokawa E, Takai S, Tanaka M, Iwase T, Suzuki M, Xiang YY, Naito Y, Yamada K, Sugimura H, Kino I. Overexpression of ERK, an EPH family receptor protein tyrosine kinase, in various human tumors. Cancer Res. 1994 Jul 15;54(14):3645–3650. [PubMed]
  • Mahone M, Saffman EE, Lasko PF. Localized Bicaudal-C RNA encodes a protein containing a KH domain, the RNA binding motif of FMR1. EMBO J. 1995 May 1;14(9):2043–2055. [PMC free article] [PubMed]
  • Marcus S, Polverino A, Barr M, Wigler M. Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7762–7766. [PMC free article] [PubMed]
  • Matsui Y, Matsui R, Akada R, Toh-e A. Yeast src homology region 3 domain-binding proteins involved in bud formation. J Cell Biol. 1996 May;133(4):865–878. [PMC free article] [PubMed]
  • Neiman AM, Stevenson BJ, Xu HP, Sprague GF, Jr, Herskowitz I, Wigler M, Marcus S. Functional homology of protein kinases required for sexual differentiation in Schizosaccharomyces pombe and Saccharomyces cerevisiae suggests a conserved signal transduction module in eukaryotic organisms. Mol Biol Cell. 1993 Jan;4(1):107–120. [PMC free article] [PubMed]
  • Pandey A, Duan H, Dixit VM. Characterization of a novel Src-like adapter protein that associates with the Eck receptor tyrosine kinase. J Biol Chem. 1995 Aug 18;270(33):19201–19204. [PubMed]
  • Ponting CP. SAM: a novel motif in yeast sterile and Drosophila polyhomeotic proteins. Protein Sci. 1995 Sep;4(9):1928–1930. [PMC free article] [PubMed]
  • Ponting CP, Phillips C. DHR domains in syntrophins, neuronal NO synthases and other intracellular proteins. Trends Biochem Sci. 1995 Mar;20(3):102–103. [PubMed]
  • Sakane F, Imai S, Kai M, Wada I, Kanoh H. Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases. J Biol Chem. 1996 Apr 5;271(14):8394–8401. [PubMed]
  • Serra-Pagès C, Kedersha NL, Fazikas L, Medley Q, Debant A, Streuli M. The LAR transmembrane protein tyrosine phosphatase and a coiled-coil LAR-interacting protein co-localize at focal adhesions. EMBO J. 1995 Jun 15;14(12):2827–2838. [PMC free article] [PubMed]
  • Stein E, Cerretti DP, Daniel TO. Ligand activation of ELK receptor tyrosine kinase promotes its association with Grb10 and Grb2 in vascular endothelial cells. J Biol Chem. 1996 Sep 20;271(38):23588–23593. [PubMed]
  • Tuzi NL, Gullick WJ. eph, the largest known family of putative growth factor receptors. Br J Cancer. 1994 Mar;69(3):417–421. [PMC free article] [PubMed]
  • Valenzuela DM, Rojas E, Griffiths JA, Compton DL, Gisser M, Ip NY, Goldfarb M, Yancopoulos GD. Identification of full-length and truncated forms of Ehk-3, a novel member of the Eph receptor tyrosine kinase family. Oncogene. 1995 Apr 20;10(8):1573–1580. [PubMed]
  • Xu Q, Alldus G, Macdonald R, Wilkinson DG, Holder N. Function of the Eph-related kinase rtk1 in patterning of the zebrafish forebrain. Nature. 1996 May 23;381(6580):319–322. [PubMed]

Articles from Protein Science : A Publication of the Protein Society are provided here courtesy of The Protein Society


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...