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J Exp Med. Jun 1, 1974; 139(6): 1473–1487.
PMCID: PMC2139691

EVIDENCE OF SUPPRESSOR CELL ACTIVITY IN SPLEENS OF MICE BEARING PRIMARY TUMORS INDUCED BY MOLONEY SARCOMA VIRUS

Abstract

Spleens from Moloney sarcoma virus (MSV) tumor-bearing C57BL/6N mice contained four times the normal number of mononuclear cells and displayed a markedly elevated "spontaneous" (mitogen-independent) DNA synthesis on a per cell basis. The number of macrophages were increased three-fold while there was a slight reduction in the percentage of T lymphocytes. The phytohemagglutinin (PHA) response on a per cell basis of spleens from tumor-bearing mice was decreased about 90% when compared with normal control mice. The primary in vitro immune response to sheep red blood cells was also suppressed to levels of less than 10% of normals. The PHA response could be restored by purification of MSV spleen cells by rayon adherence columns and by removal of phagocytic cells by an iron/magnet technique. The activity of suppressor cells in MSV spleens was demonstrated in mixtures with syngeneic normal spleen cells where a marked impairment of the PHA response was observed. Spleen cells from tumor-free nude mice and normal spleen cells treated by anti-θ serum plus guinea pig complement (C'), both totally unreactive to PHA, had no such effect. The inhibitor cell in MSV spleens was shown to be insensitive to inactivation by anti-θ plus C', but could be removed by the adherence columns and the iron/magnet technique. These data suggest that this suppressor cell is a cell of the monocyte/macrophage series. Suggestive evidence was also presented that the suppressor cells belong to a proliferating population in MSV spleens. Similar suppressor cells have been previously demonstrated in spleens of mice during a variety of immune responses. Our data show, that a tumor, although stimulating the immune system, nevertheless may be suppressive on certain immune functions through the activation of suppressor cells.

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Selected References

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  • BURNET M. Cancer: a biological approach. III. Viruses associated with neoplastic conditions. IV. Practical applications. Br Med J. 1957 Apr 13;1(5023):841–847. [PMC free article] [PubMed]
  • Klein G. Experimental studies in tumor imminology. Fed Proc. 1969 Nov-Dec;28(6):1739–1753. [PubMed]
  • Smith RT. Possibilities and problems of immunologic intervention in cancer. N Engl J Med. 1972 Aug 31;287(9):439–450. [PubMed]
  • Lavrin DH, Herberman RB, Nunn M, Soares N. In vitro cytotoxicity studies of murine sarcoma virus-induced immunity in mice. J Natl Cancer Inst. 1973 Nov;51(5):1497–1508. [PubMed]
  • Hersh EM, Oppenheim JJ. Impaired in vitro lymphocyte transformation in Hodgkin's disease. N Engl J Med. 1965 Nov 4;273(19):1006–1012. [PubMed]
  • Garrioch DB, Good RA, Gatti RA. Lymphocyte response to P.H.A. in patients with non-lymphoid tumours. Lancet. 1970 Mar 21;1(7647):618–618. [PubMed]
  • Sample WF, Gertner HR, Jr, Chretien PB. Inhibition of phytohemagglutinin-induced in vitro lymphocyte transformation by serum from patients with carcinoma. J Natl Cancer Inst. 1971 Jun;46(6):1291–1297. [PubMed]
  • Häyry P, Rago D, Defendi V. Inhibition of phytohemagglutinin- and alloantigen-induced lymphocyte stimulation by Rauscher leukemia virus. J Natl Cancer Inst. 1970 Jun;44(6):1311–1319. [PubMed]
  • McCoy JL, Fefer A, Glynn JP. Comparative studies on the induction of transplantation resistance in BALB-c and C57BL-6 mice in three murine leukemia systems. Cancer Res. 1967 Oct;27(10):1743–1748. [PubMed]
  • REIF AE, ALLEN JM. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. [PMC free article] [PubMed]
  • Rosenstreich DL, Nowotny A, Chused T, Mergenhagen SE. In vitro transformation of mouse bone-marrow-derived (B) lymphocytes induced by the lipid component of endotoxin. Infect Immun. 1973 Sep;8(3):406–411. [PMC free article] [PubMed]
  • Zoschke DC, Bach FH. Specificity of antigen recognition by human lymphocytes in vitro. Science. 1970 Dec 25;170(3965):1404–1406. [PubMed]
  • Mishell RI, Dutton RW. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. [PMC free article] [PubMed]
  • Cunningham AJ, Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed]
  • Risdall RJ, Aust JC, McKhann CF. Immune capacity and response to antigenic tumors. Cancer Res. 1973 Sep;33(9):2078–2085. [PubMed]
  • Allison AC, Denman AM, Barnes RD. Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet. 1971 Jul 17;2(7716):135–140. [PubMed]
  • Droege W. Amplifying and suppressive effect of thymus cells. Nature. 1971 Dec 31;234(5331):549–551. [PubMed]
  • Baker PJ, Stashak PW, Amsbaugh DF, Prescott B, Barth RF. Evidence for the existence of two functionally distinct types of cells which regulate the antibody response to type 3 pneumococcal polysaccharide. J Immunol. 1970 Dec;105(6):1581–1583. [PubMed]
  • Gershon RK, Cohen P, Hencin R, Liebhaber SA. Suppressor T cells. J Immunol. 1972 Mar;108(3):586–590. [PubMed]
  • Chused TM, Steinberg AD, Parker LM. Enhanced antibody response of mice to polyinosinic-polycytidylic acid by antithymocyte serum and its age-dependent loss in NZB-W mice. J Immunol. 1973 Jul;111(1):52–57. [PubMed]
  • Parkhouse RM, Dutton RW. Inhibition of spleen cell DNA synthesis by autologous macrophages. J Immunol. 1966 Nov;97(5):663–669. [PubMed]
  • Scott MT. Biological effects of the adjuvant Corynebacterium parvum. I. Inhibition of PHA, mixed lymphocyte and GVH reactivity. Cell Immunol. 1972 Nov;5(3):459–468. [PubMed]
  • Folch H, Yoshinaga M, Waksman BH. Regulation of lymphocyte responses in vitro. 3. Inhibition by adherent cells of the T-lymphocyte response to phytohemagglutinin. J Immunol. 1973 Mar;110(3):835–839. [PubMed]
  • Everson LK, Buell DN, Rogentine GN., Jr Separation of human lymphoid cells into G 1 , S, and G 2 cell cycle populations by use of a velocity sedimentation technique. J Exp Med. 1973 Feb 1;137(2):343–358. [PMC free article] [PubMed]
  • Leclerc JC, Gomard E, Plata F, Levy JP. Cell-mediated immune reaction against tumors induced by oncornaviruses. II. Nature of the effector cells in tumor-cell cytolysis. Int J Cancer. 1973 Mar 15;11(2):426–432. [PubMed]
  • Herberman RB, Nunn ME, Lavrin DH, Asofsky R. Effect of antibody to theta antigen on cell-mediated immunity induced in syngeneic mice by murine sarcoma virus. J Natl Cancer Inst. 1973 Nov;51(5):1509–1512. [PubMed]
  • Andersson J, Möller G, Sjöberg O. Selective induction of DNA synthesis in T and B lymphocytes. Cell Immunol. 1972 Aug;4(4):381–393. [PubMed]
  • Janossy G, Greaves MF. Lymphocyte activation. I. Response of T and B lymphocytes to phytomitogens. Clin Exp Immunol. 1971 Oct;9(4):483–498. [PMC free article] [PubMed]
  • Vann DC, Kettman JR. In vitro cooperation of cells of bone marrow and thymus origins in the generation of antibody-forming cells. J Immunol. 1972 Jan;108(1):73–80. [PubMed]
  • Rowland GF, Edwards AJ, Sumner MR, Hurd CM. Thymic dependency of tumor-induced immunodepression. J Natl Cancer Inst. 1973 May;50(5):1329–1336. [PubMed]
  • Sjöberg O. Effect of allogeneic cell interaction on the primary immune response in vitro. Cell types involved in suppression and stimulation of antibody synthesis. Clin Exp Immunol. 1972 Nov;12(3):365–375. [PMC free article] [PubMed]

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