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J Exp Med. Jun 1, 1970; 131(6): 1190–1199.
PMCID: PMC2138848

AUSTRALIA ANTIGEN (A HEPATITIS-ASSOCIATED ANTIGEN)

PURIFICATION AND PHYSICAL PROPERTIES

Abstract

Australia antigen [Au(1)], a particle associated with viral hepatitis, was isolated from the plasma of a patient with chronic anicteric hepatitis and leukemia who had received radioactive phosphorus. We have found that the immunoreactivity and appearance of Au(1) in the electron microscope were not altered by treatment with enzymes including trypsin, pronase, lipase, phospholipase C, ribonuclease, deoxyribonuclease, amylase, and neuraminidase. In contrast, other serum constituents were degraded by these enzymes. Therefore, treatment of the patient's plasma with many enzymes was exploited as an initial step for the isolation of Au(1). Subsequently, Au(1) was purified from the enzyme-treated 32P-labeled plasma by gel filtration through Sephadex G-200 and centrifugation through sucrose and in cesium chloride gradients. There were no detectable human serum components in the purest fractions, as tested by immunoelectrophoresis and immunodiffusion. The density of the purified Au(1) was 1.21 in CsCl. The particle measured about 200 A in diameter, was predominantly spherical in shape and appeared to be composed of subunits. Nucleic acids were not detected by spectrophotometric, radiochemical, and chemical analyses. Immunoreactivity of purified Au(1) was destroyed by heating for 1 hr at 85°C but was stable at 56°C. Treatment with Carnoy's solution (3 parts ethanol:1 part glacial acetic acid) followed by pronase disrupted the particles as seen with the electron microscope. These findings, combined with other published information on Australia antigen and viral hepatitis, suggest that the bulk of Australia antigen in the blood of this patient is an incomplete virus or virus capsid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BLUMBERG BS. POLYMORPHISMS OF THE SERUM PROTEINS AND THE DEVELOPMENT OF ISO-PRECIPITINS IN TRANSFUSED PATIENTS. Bull N Y Acad Med. 1964 May;40:377–386. [PMC free article] [PubMed]
  • Blumberg BS, Sutnick AI, London WT. Hepatitis and leukemia: their relation to Australia antigen. Bull N Y Acad Med. 1968 Dec;44(12):1566–1586. [PMC free article] [PubMed]
  • Bayer ME, Blumberg BS, Werner B. Particles associated with Australia antigen in the sera of patients with leukaemia, Down's Syndrome and hepatitis. Nature. 1968 Jun 15;218(5146):1057–1059. [PubMed]
  • Millman I, Zavatone V, Gerstley BJ, Blumberg BS. Australia antigen detected in the nuclei of liver cells of patients with viral hepatitis by the fluorescent antibody technic. Nature. 1969 Apr 12;222(5189):181–184. [PubMed]
  • Blumberg BS, Gerstley BJ, Hungerford DA, London WT, Sutnick AI. A serum antigen (Australia antigen) in Down's syndrome, leukemia, and hepatitis. Ann Intern Med. 1967 May;66(5):924–931. [PubMed]
  • Sutnick AI, London WT, Gerstley BJ, Cronlund MM, Blumberg BS. Anicteric hepatitis associated with Australia antigen. Occurrence in patients with Down's syndrome. JAMA. 1968 Sep 2;205(10):670–674. [PubMed]
  • Okochi K, Murakami S. Observations on Australia antigen in Japanese. Vox Sang. 1968;15(5):374–385. [PubMed]
  • Prince AM. An antigen detected in the blood during the incubation period of serum hepatitis. Proc Natl Acad Sci U S A. 1968 Jul;60(3):814–821. [PMC free article] [PubMed]
  • Prince AM. Relation of Australia and SH antigens. Lancet. 1968 Aug 24;2(7565):462–463. [PubMed]
  • Hirschman RJ, Shulman NR, Barker LF, Smith KO. Virus-like particles in sera of patients with infectious and serum hepatitis. JAMA. 1969 Jun 2;208(9):1667–1670. [PubMed]
  • Gocke DJ, Kavey NB. Hepatitis antigen. Correlation with disease and infectivity of blood-donors. Lancet. 1969 May 31;1(7605):1055–1059. [PubMed]
  • London WT, Sutnick AI, Blumberg BS. Australia antigen and acute viral hepatitis. Ann Intern Med. 1969 Jan;70(1):55–59. [PubMed]
  • Alter HJ, Blumberg BS. Further studies on a "new" human isoprecipitin system (Australia antigen). Blood. 1966 Mar;27(3):297–309. [PubMed]
  • KISSANE JM, ROBINS E. The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J Biol Chem. 1958 Jul;233(1):184–188. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • MARTIN RG, AMES BN. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed]

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