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J Bacteriol. 1986 Dec; 168(3): 1466–1467.
PMCID: PMC213663

Determination of bacterial cell volume with the Coulter Counter.


Two methods were used to determine mean volumes of cells of Escherichia coli B/rA in both stationary- and exponential-phase cultures, i.e., electronic measurement with a Coulter Counter-Analyzer system and biophysical measurement of the total volume and number of cells in sedimented cell pellets. Within experimental errors, the methods gave the same mean cell volumes.

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Selected References

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  • Kubitschek HE. Constancy of the ratio of DNA to cell volume in steady-state cultures of Escherichia coli B-r. Biophys J. 1974 Feb;14(2):119–123. [PMC free article] [PubMed]
  • Kubitschek HE. Increase in cell mass during the division cycle of Escherichia coli B/rA. J Bacteriol. 1986 Nov;168(2):613–618. [PMC free article] [PubMed]
  • Kubitschek HE, Bendigkeit HE, Loken MR. Onset of DNA synthesis during the cell cycle in chemostat cultures. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1611–1617. [PMC free article] [PubMed]
  • Stock JB, Rauch B, Roseman S. Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem. 1977 Nov 10;252(21):7850–7861. [PubMed]

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