• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Dec 1986; 168(3): 1155–1158.
PMCID: PMC213616

Heat shock regulatory gene rpoH mRNA level increases after heat shock in Escherichia coli.

Abstract

The Escherichia coli rpoH gene product sigma 32 is essential for the increase in heat shock gene transcription found after exposure of the bacteria to a sudden temperature increase. It is not known how the concentration of active sigma 32 is modulated. We showed that rpoH transcript levels increased after heat shock and that the magnitude of the increase in the level of mRNA was correlated with the magnitude of the temperature shift. The increase in the level of rpoH mRNA was still found in rpoH mutants so the mechanism of induction differed from that of the set of previously identified heat shock genes. The increased concentration of rpoH mRNA should result in a higher level of sigma 32, which is likely to be important for increasing heat shock gene transcription.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ang D, Chandrasekhar GN, Zylicz M, Georgopoulos C. Escherichia coli grpE gene codes for heat shock protein B25.3, essential for both lambda DNA replication at all temperatures and host growth at high temperature. J Bacteriol. 1986 Jul;167(1):25–29. [PMC free article] [PubMed]
  • Bardwell JC, Craig EA. Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous. Proc Natl Acad Sci U S A. 1984 Feb;81(3):848–852. [PMC free article] [PubMed]
  • Bardwell JC, Tilly K, Craig E, King J, Zylicz M, Georgopoulos C. The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein. J Biol Chem. 1986 Feb 5;261(4):1782–1785. [PubMed]
  • Barry G, Squires C, Squires CL. Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3331–3335. [PMC free article] [PubMed]
  • Bloom M, Skelly S, VanBogelen R, Neidhardt F, Brot N, Weissbach H. In vitro effect of the Escherichia coli heat shock regulatory protein on expression of heat shock genes. J Bacteriol. 1986 May;166(2):380–384. [PMC free article] [PubMed]
  • Collins JJ, Roberts GP, Brill WJ. Posttranscriptional control of Klebsiella pneumoniae nif mRNA stability by the nifL product. J Bacteriol. 1986 Oct;168(1):173–178. [PMC free article] [PubMed]
  • Cooper S, Ruettinger T. A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12. Mol Gen Genet. 1975 Aug 5;139(2):167–176. [PubMed]
  • Cowing DW, Bardwell JC, Craig EA, Woolford C, Hendrix RW, Gross CA. Consensus sequence for Escherichia coli heat shock gene promoters. Proc Natl Acad Sci U S A. 1985 May;82(9):2679–2683. [PMC free article] [PubMed]
  • Craig EA. The heat shock response. CRC Crit Rev Biochem. 1985;18(3):239–280. [PubMed]
  • Drahos DJ, Hendrix RW. Effect of bacteriophage lambda infection on synthesis of groE protein and other Escherichia coli proteins. J Bacteriol. 1982 Mar;149(3):1050–1063. [PMC free article] [PubMed]
  • Georgopoulos CP. Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2977–2981. [PMC free article] [PubMed]
  • Gorski K, Roch JM, Prentki P, Krisch HM. The stability of bacteriophage T4 gene 32 mRNA: a 5' leader sequence that can stabilize mRNA transcripts. Cell. 1985 Dec;43(2 Pt 1):461–469. [PubMed]
  • Gross CA, Grossman AD, Liebke H, Walter W, Burgess RR. Effects of the mutant sigma allele rpoD800 on the synthesis of specific macromolecular components of the Escherichia coli K12 cell. J Mol Biol. 1984 Jan 25;172(3):283–300. [PubMed]
  • Grossman AD, Erickson JW, Gross CA. The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell. 1984 Sep;38(2):383–390. [PubMed]
  • Herendeen SL, VanBogelen RA, Neidhardt FC. Levels of major proteins of Escherichia coli during growth at different temperatures. J Bacteriol. 1979 Jul;139(1):185–194. [PMC free article] [PubMed]
  • Jinks-Robertson S, Gourse RL, Nomura M. Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons. Cell. 1983 Jul;33(3):865–876. [PubMed]
  • Kochan J, Murialdo H. Stimulation of groE synthesis in Escherichia coli by bacteriophage lambda infection. J Bacteriol. 1982 Mar;149(3):1166–1170. [PMC free article] [PubMed]
  • Krueger JH, Walker GC. groEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR+-dependent fashion. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1499–1503. [PMC free article] [PubMed]
  • Landick R, Vaughn V, Lau ET, VanBogelen RA, Erickson JW, Neidhardt FC. Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor. Cell. 1984 Aug;38(1):175–182. [PubMed]
  • Lemaux PG, Herendeen SL, Bloch PL, Neidhardt FC. Transient rates of synthesis of individual polypeptides in E. coli following temperature shifts. Cell. 1978 Mar;13(3):427–434. [PubMed]
  • Neidhardt FC, VanBogelen RA. Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem Biophys Res Commun. 1981 May 29;100(2):894–900. [PubMed]
  • Neidhardt FC, VanBogelen RA, Lau ET. Molecular cloning and expression of a gene that controls the high-temperature regulon of Escherichia coli. J Bacteriol. 1983 Feb;153(2):597–603. [PMC free article] [PubMed]
  • Neidhardt FC, VanBogelen RA, Vaughn V. The genetics and regulation of heat-shock proteins. Annu Rev Genet. 1984;18:295–329. [PubMed]
  • Osawa T, Yura T. Effects of reduced amount of RNA polymerase sigma factor on gene expression and growth of Escherichia coli: studies of the rpoD450 (amber) mutation. Mol Gen Genet. 1981;184(2):166–173. [PubMed]
  • Taylor WE, Straus DB, Grossman AD, Burton ZF, Gross CA, Burgess RR. Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase. Cell. 1984 Sep;38(2):371–381. [PubMed]
  • Tilly K, McKittrick N, Zylicz M, Georgopoulos C. The dnaK protein modulates the heat-shock response of Escherichia coli. Cell. 1983 Sep;34(2):641–646. [PubMed]
  • Tobe T, Ito K, Yura T. Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli. Mol Gen Genet. 1984;195(1-2):10–16. [PubMed]
  • Yamamori T, Yura T. Temperature-induced synthesis of specific proteins in Escherichia coli: evidence for transcriptional control. J Bacteriol. 1980 Jun;142(3):843–851. [PMC free article] [PubMed]
  • Yamamori T, Yura T. Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1982 Feb;79(3):860–864. [PMC free article] [PubMed]
  • Yura T, Tobe T, Ito K, Osawa T. Heat shock regulatory gene (htpR) of Escherichia coli is required for growth at high temperature but is dispensable at low temperature. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6803–6807. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...