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J Cell Biol. Jun 29, 1998; 141(7): 1529–1537.
PMCID: PMC2133009

G Protein β Subunit–null Mutants Are Impaired in Phagocytosis and Chemotaxis Due to Inappropriate Regulation of the Actin Cytoskeleton

Abstract

Chemotaxis and phagocytosis are basically similar in cells of the immune system and in Dictyostelium amebae. Deletion of the unique G protein β subunit in D. discoideum impaired phagocytosis but had little effect on fluid-phase endocytosis, cytokinesis, or random motility. Constitutive expression of wild-type β subunit restored phagocytosis and normal development. Chemoattractants released by cells or bacteria trigger typical transient actin polymerization responses in wild-type cells. In β subunit–null cells, and in a series of β subunit point mutants, these responses were impaired to a degree that correlated with the defect in phagocytosis. Image analysis of green fluorescent protein–actin transfected cells showed that β subunit– null cells were defective in reshaping the actin network into a phagocytic cup, and eventually a phagosome, in response to particle attachment. Our results indicate that signaling through heterotrimeric G proteins is required for regulating the actin cytoskeleton during phagocytic uptake, as previously shown for chemotaxis. Inhibitors of phospholipase C and intracellular Ca2+ mobilization inhibited phagocytosis, suggesting the possible involvement of these effectors in the process.

In chemotaxis, ameboid cells, like leukocytes and Dictyostelium cells, respond directionally to chemical gradients; in phagocytosis, they bind and engulf foreign organisms or apoptotic cells (Devreotes and Zigmond, 1988; Rabinovitch, 1995). Chemotaxis and phagocytosis seem to be closely related, suggesting that the underlying signal transduction events and cytoskeletal responses have evolved in parallel (Metchnikoff, 1968). In the simple eukaryote Dictyostelium discoideum, and in ameboid cells of the immune systems of animals, chemotactic and phagocytic stimuli elicit a remarkably similar spectrum of behavioral events and biochemical reactions (Devreotes and Zigmond, 1988; Greenberg, 1995). Foremost among these is the polymerization of actin into filaments that support the extension of pseudopods and the formation of phagocytic cups (McRobbie and Newell, 1983; Greenberg, 1995; Zigmond, 1996).

Chemotaxis and phagocytosis involve both G protein– coupled and tyrosine kinase-linked signal transduction pathways. Many chemoattractants interact with serpentine receptors, such as cAMP receptors in Dictyostelium and chemokine receptors in leukocytes (Parent and Devreotes, 1996; Murphy, 1996). Agonists for receptor tyrosine kinases trigger actin polymerization and act as chemoattractants (Kundra et al., 1994). With regard to phagocytosis, bound particles activate protein tyrosine kinases, such as syk, leading to actin polymerization and rearrangement, possibly through involvement of the small G protein Rho (Greenberg et al., 1994, 1996; Indik et al., 1995; Hackam et al., 1997). Heterotrimeric G proteins have been involved in chemotactic activation of macrophages, which leads to phagocytosis (Thelen and Wirthmueller, 1994), and in phagosome-endosome fusion (Desjardins et al., 1994; Béron et al., 1995; Allen and Aderem, 1996), whereas no compelling evidence has been reported so far for a role of G proteins in phagocytic uptake.

D. discoideum amebae contain a single G protein β subunit; its deletion creates cells that lack functional G proteins (Lilly et al., 1993; Wu et al., 1995). These mutants are severely defective in chemotaxis, aggregation, and development. When plated on bacterial lawns, they form smooth plaques consisting of monolayers of undifferentiated cells. These plaques are much smaller than those of wild type (Wu et al., 1995). We report here that this slow growth reflects a severe defect in phagocytosis, which is primarily due to a failure in organizing the actin meshwork into a phagocytic cup.We have also used several inhibitors of G protein–linked effectors, such as protein kinase A (PKA),1 protein kinase C (PKC), and phospholipase C (PLC) tyrosine kinases, phosphoinositide 3-kinase (PI3) and phosphoinositide 4-kinase (PI4), as well as signal transduction mutants, to dissect the downstream components involved in phagocytosis. We suggest that a signaling pathway mediated by heterotrimeric G proteins, possibly involving PLC activation and mobilization of Ca2+ ions, is necessary to regulate the actin cytoskeleton during phagocytosis.

Materials and Methods

Cell Cultures

The following D. discoideum strains were used throughout: wild-type strains AX2 and AX3; β subunit–null strains LW6 and LW14, or β subunit point mutants S1, S2, S3, I1, I2; “rescue” mutants LW18 and LW20. Transformation, selection, and developmental phenotype of the mutants have been described by Lilly et al. (1993) and Wu et al. (1995).

For axenic growth, cells were cultured in AX2 medium (Watts and Ashwort, 1972) under shaking at 150 rpm and 23°C. G418 at a concentration of 20 μg/ml was added to cultures of LW14, point mutants, LW18, and LW20. For starvation, cells were washed twice in 0.017 M Na-K Soerensen phosphate buffer, pH 6.0, and then shaken in the same buffer at a concentration of 107 per ml.

Phagocytosis Microassays

Phagocytosis was measured by a modification of a previously described assay (Bozzaro et al., 1987), using cells starved for 30 min. Cells were vortexed and mixed with cold FITC-labeled Escherichia coli B/r, or in some cases Salmonella minnesota R595, at a final concentration of 4 × 106 cells and 5 × 109 bacteria per ml, respectively, in a final volume of 0.1 ml in 5-ml polystyrene tubes. The tubes were placed on a rack mounted on a vortex equipped with speed control (model Reax 2000; Heidolph, Kelheim, Germany), and shaken at 200 vibrations per minute. At various time points, cells were washed free of unbound bacteria by dilution with 5 ml of cold 0.050 M Na-phosphate buffer, pH 9.2, and centrifugation at 110 g for 3 min. After additional washing, the cell pellet was resuspended in 1 ml of Na-phosphate buffer containing 0.2% Triton X-100 and then lysed for 30 min. Fluorescence in cell lysates was measured in a spectrofluorimeter (model SFM25; Kontron, Schliereu, Switzerland) at an excitation wavelength of 470 nm and emission of 520 nm. To determine the number of bacteria, a calibration curve was made by serial dilutions of FITC-labeled bacteria lysed with 1% SDS for 2 min at 90°C (Vogel, 1987).

For measuring phagocytosis of latex beads, cells at a final concentration of 4 × 106 per ml were mixed with a suspension of 1-μm-diam standard Dow latex beads (Serva, Heidelberg, Germany) at a final OD of 1.5, in a total volume of 0.1 ml Soerensen phosphate buffer. At the end of incubation, cells were washed free of beads by dilution and washing as above. The cell pellet was resuspended in 1-ml Soerensen phosphate buffer containing Triton X-100, lysed, and then the OD of the suspension was measured. The percentage of engulfed beads was determined by reference to a serial dilution curve of latex beads.

Phagocytosis of yeasts was studied under similar conditions, by mixing 2 × 106 cells per ml with a fivefold excess of TRITC-labeled, heat-killed yeast particles. At the end of the incubation, 0.9 ml of cold Soerensen phosphate buffer was added to each sample, followed by the addition of 0.1 ml of trypan blue (2 mg/ml in 0.02 M citrate buffer containing 0.15 M NaCl) for quenching the fluorescence of noningested particles (Hed, 1986; Maniak et al., 1995). After 15 min of incubation under shaking, excess trypan blue was removed by centrifugation and washing, and then fluorescence determined at 544/577 nm in the spectrofluorimeter.

FITC bacteria were freshly prepared by incubating the bacteria at a final concentration of 1010/ml with 0.1 mg/ml FITC (Sigma Chemical Co., St. Louis, MO) as described (Vogel, 1987), except that the incubation time and temperature were 90 min and 23°C. TRITC yeasts were prepared by labeling 2 × 1010 heat-killed yeast particles in 20 ml of 0.05 M Na2HPO4, pH 9.2, containing 2 mg/ml TRITC (Sigma Chemical Co.) and incubating at 37°C for 30 min under shaking. After extensive washing, the TRITC yeasts were aliquoted in 0.017 M Na-K Soerensen phosphate buffer, pH 6.0, and stored at −20°C until use.

Growth Assays on Bacteria or Axenic Medium

For measuring growth on shaken bacteria, cells were inoculated at 5 × 104 per ml in a suspension of 1010 E. coli B/r per ml, and cell number was counted every 3 h for a total of 36 h. Colony growth on bacterial lawns was assessed by inoculating cells with a toothpick on a lawn of E.coli B/2 cultivated on nutrient agar and then measuring the diameter of the plaque every 12 h for a total of 48 h.

For growth in axenic medium, growing cells were diluted to 105 per ml in fresh axenic medium and counted every 8 h for a total of 56 h.

Fluid-Phase Endocytosis Assay

Fluid-phase endocytosis was measured as described by Aubry et al. (1993) using FITC-labeled dextran (70,000; Sigma Chemical Co.) as a marker, except that the final volume was 0.1 ml per time point.

Actin Polymerization Assay

Chemoattractant-induced F-actin formation was measured as described (Hall et al., 1988). Briefly, cells were shaken at 2 × 107/ml in Soerensen phosphate buffer. At various time points after addition of 10−6 M cAMP or supernatant from bacterial growth medium, 0.1-ml cell suspension was transferred to 0.9-ml stop solution containing 3.7% formaldehyde, 0.1% Triton X-100, 0.25 μM TRITC-phalloidin in 20 mM K-PO4, 10 mM Pipes, 5 mM EGTA, 2 mM MgCl2, pH 6.8. After staining for 1 h, samples were centrifuged for 5 min in a microfuge (model Biofuge A; Heraeus, Hanau, Germany), pellets were extracted with 1 ml methanol for 20 h, and then fluorescence (540/575 nm) was read in a spectrofluorimeter. Bacteria-conditioned medium was prepared by clarification of a stationary phase culture of Klebsiella aerogenes grown at 22°C. Medium was used as a stimulus at a dilution of 1:100.

Transfection with Green Fluorescent Protein–Actin

Fusion of the coding region of the D. discoideum actin with the red-shifted green fluorescent protein (GFP) S65T mutant has been described (Westphal et al., 1997). Two vectors for expression of the GFP–actin fusion were constructed, one in which the fusion product was inserted in the pDEX H vector containing the resistance to G418 (Faix et al., 1992), and a second based on the pBsr2 vector containing the resistance to blasticidin (Sutoh, 1993). The first vector was used for transforming AX2, AX3, and LW6, and the second vector for LW20, which is already resistant to G418. Transformation was done by electroporation and transformants were selected on plates in nutrient medium containing 20 μg/ml G418 or 10 μg/ml blasticidin. Individual clones were used in the experiments.

Light and Fluorescence Microscopy of Living and Fixed Cells

Phagocytosis of yeast particles by single cells incubated on Petriperm dishes (no. 26136906; Heraeus) was followed for 2 h by time-lapse videomicroscopy using an Axiovert microscope (model 35; Carl Zeiss, Inc., Oberkochen, Germany) equipped with a 100× Neofluar objective and a Zeiss charge-coupled device videocamera (model ZVS-47DE) connected to a Panasonic videorecorder (model 6050; Osaka, Japan). Images were recorded at an interval of 0.5 s.

Cells incubated with TRITC-labeled yeast particles on glass coverslips were fixed with picric acid and formaldehyde and postfixed with 70% ethanol as described by Humbel and Biegelmann (1992). The fixed cells were labeled with 0.5 μg/ml FITC-phalloidin (Sigma Chemical Co.) for 30 min at room temperature, and confocal sections taken on an inverted Zeiss microscope (model LSM 410) with a 100× Neofluar 1.3 oil-immersion objective. For excitation, the 488-nm band of an argon-ion laser line was used and its emission collected with a 510–525-nm bandpass filter for FITC and a longpass filter of 570 nm for TRITC.

Confocal serial images of living cells expressing GFP–actin and incubated with TRITC-labeled yeast particles on glass coverslips were obtained by scanning at intervals of 34 s, using a 100× Plan-Neofluar objective. To avoid damaging the cells upon intense light exposure, the laser intensity was attenuated to 1/100 of its maximal power. Since the yeast particles were strongly labeled, the small fraction of their emission obtained by excitation as 448 nm was sufficient to record their fluorescence together with that of GFP. The two contributions to the emission signal could be separated by using a bandpass filter of 510–525 nm for GFP and a longpass filter of 570 nm for TRITC. Phase contrast was recorded simultaneously. The images from the green channel in Fig. Fig.77 were pseudocolored with a color code ranging from cyan to yellow, and then superimposed to the red channel using Applied Visualization Systems (Waltham, PA) software.

Figure 7
Phagocytosis and actin dynamics in GFP–actin-transfected wild-type (A and B) and β-null (C–F) cells. Each panel (A–F) shows a series of images recorded at intervals of 34 s. Phase-contrast and corresponding GFP–actin ...

Nuclei of cells growing in axenic medium were labeled with 4′,6-diamidino-2-phenylindole (Sigma Chemical Co.) after cell fixation and postfixation as above. The number of nuclei per cell was determined on a Leitz fluorescence microscope (model Laborlox D; Wetzlar, Germany) using a LH102Z filter.

Results

Phagocytosis and Growth on Bacteria Are Impaired in β Subunit–null cells and Restored in Rescue Mutants

Phagocytosis was assessed in wild-type and in β subunit– null () cells by measuring the uptake rate of fluorescently-labeled E. coli B/r or latex beads. Shaken wild-type cells engulfed ~300 bacteria per hour under our conditions, a value in the range of previously published data (Gerisch, 1959; Vogel et al., 1980; Bozzaro et al., 1987). In cells lacking the G protein β subunit, the initial uptake rate of bacteria or latex beads was decreased to 20 or 40–60% of the wild-type rate, respectively (Fig. (Fig.1,1, A and B). Constitutive expression of wild-type β subunit cDNA in cells restored nearly normal rates of phagocytosis (Fig. (Fig.1,1, A and B). Replacing E. coli B/r with Salmonella minnesota R595, which is known to adhere stronger to the surface of Dictyostelium cells (Malchow et al., 1967; Bozzaro and Gerisch, 1978; Niewöhner et al., 1997), did not significantly alter the uptake rates of β subunit–null mutants, rescue mutants, or wild-type cells (data not shown). It is open to what extent uptake of different types of bacteria, or of inert particles, such as latex beads, is mediated by different receptors in Dictyostelium, though some genetic evidence favors the hypothesis that both specific, lectin-type receptors and nonspecific receptors contribute to phagocytosis (Vogel et al., 1980; Bozzaro and Ponte, 1995; Chia, 1996). The defect in phagocytosis of cells seems, however, to be independent of particle binding to a particular receptor, since the uptake of E.coli, S. minnesota R595 as well as latex beads was inhibited in the mutant and restored in rescue cells.

Figure 1Figure 1
G protein β subunit stimulates phagocytosis, but not pinocytosis. Phagocytosis of (A) FITC-labeled E. coli B/r or (B) 1-μm-diam latex beads were assayed by incubating cells under shaking with a 1,000- or a 200-fold excess of bacteria ...

We measured growth of and rescue cells both in shaken suspension or on a lawn of E. coli. Consistent with the phagocytosis defect, the doubling time on shaken suspensions was increased from 3 to 10 h in cells and the rate of plaque expansion on bacterial lawns was decreased from 0.6 to 0.1 mm/h. Normal rates of growth under both conditions were found for the rescue cells (Table (TableI).I).

Table I
Correlation between Chemoattractant-induced F Actin Assembly, Phagocytosis, and Growth on Bacteria

We also examined individual clones expressing different randomly mutagenized β subunit cDNAs. None of the mutant proteins rescued the developmental defects of the cells; the individual clones all formed smooth plaques. Rates of phagocytosis and doubling times in suspensions of E. coli closely correlated with their plaque sizes (Table (TableI),I), presumably depending on the severity of the mutation. The diameters of these plaques were small like those of the cells, intermediate, or large like those of rescued mutants (Table (TableII).

Pinocytosis, Cytokinesis, and Motility Are Normal in β Subunit–null Cells

Although the cells were severely defective in chemotaxis and phagocytosis, they were competent in cytokinesis, pinocytosis, and random motility. Pinocytosis was directly monitored by measuring uptake of FITC-conjugated dextran. The initial rates of uptake were essentially identical in mutant, wild-type, and rescued cells (Fig. (Fig.11 C). Doubling times and cytokinesis of and wild-type cells in liquid media were also similar (Table (TableI).I). We also monitored the random motility of undifferentiated cells on hydrophilic Petriperm surfaces and found that it was 3.9 (±1.6 SD) and 3.6 (±1.8 SD) μm/min for wild-type and cells, respectively. Finally, no differences in cell adhesion to polystyrene surface were detected between undifferentiated and control cells (data not shown). Taken together, these results strongly indicate that the small plaque size of cells on bacterial lawns is solely due to a defect in phagocytosis and not to additional defects in cytokinesis or motility.

The Phagocytosis Defect Is Correlated with Impaired F-actin Formation in Response to Chemoattractants

Chemoattractants induce rapid, transient increases in cellular levels of filamentous actin (McRobbie and Newell, 1983; Hall et al., 1988; Tardif et al., 1995). As previously reported, cells are not attracted to folic acid, cAMP, or bacteria-conditioned medium containing a broad range of chemoattractants. Overexpression of the surface receptor cAR1 does not restore sensitivity to cAMP (Lilly et al., 1993). Consistent with these results, the actin polymerization responses triggered by these compounds are undetectable in cells (Fig. (Fig.2).2). We also examined the series of β subunit point mutants for actin polymerization elicited by bacteria-conditioned medium or cAMP. The magnitudes of these responses correlated with the rates of phagocytosis as well as growth rates on shaken suspensions or lawns of bacteria (Table (TableI;I; Fig. Fig.33 and data not shown).

Figure 2
G protein β subunit is required for chemoattractant-induced actin polymerization. F-actin formation in suspended cell cultures following addition of: (A) supernatants from bacterial growth medium or (B) 10−6 M cAMP. Closed triangles ...
Figure 3
Phagocytosis correlates with rates of F-actin formation in response to chemoattractants. The data reported in Table TableI,I, columns 6 and 7 for cells, point mutants, rescue cells, and wild-type were plotted to determine ...

The β Subunit–null Cells Are Defective in Reshaping the Actin Cytoskeleton into a Phagocytic Cup

The correlation between actin polymerization and bacteria uptake prompted us to examine actin localization during phagocytosis in cells. Labeling with phalloidin or anti-actin mAbs failed to reveal major differences in actin distribution between , rescue, or wild-type cells incubated with bacteria (data not shown). Consistent with the reduced uptake rates of particles, cells contained fewer bacteria in their cytoplasm, some of which were surrounded by actin, probably representing freshly ingested phagosomes.

To recognize details, we replaced bacteria by the larger heat-killed yeasts. Like uptake of bacteria, engulfment of suspended yeast was strongly inhibited in the mutant (Fig. (Fig.4).4). Yeast uptake by cells incubated on a hydrophilic Petriperm surface was recorded. In parallel, cells were fixed at different times and labeled with FITC-phalloidin. A serial image sequence of individual wild-type or cells incubated with yeast particles is shown in Fig. Fig.5.5. Both cell types extended leading edges, and bound yeast particles, which they came in contact with, without detectable differences. Binding either led to formation of a phagocytic cup around a particle or was followed by detachment. Similarly, when a phagocytic cup was formed, the cup could progress up to engulfment of the particle or a new leading front was formed, with subsequent cell detachment from the yeast particle. This behavior is consistent with a zipper model of phagocytosis (Swanson and Baer, 1995), which has been shown to apply to Dictyostelium (Maniak et al., 1995). All these events occurred in wild-type and cells, but with significantly different probabilities. As shown in Table TableII,II, the number of yeast–cell attachment events leading to successful phagocytic cup formation and engulfment was strongly reduced in cells. The mutant was defective in both steps, phagocytic cup, and phagosome formation, with an inhibition of 35 and 80%, respectively, compared with wild-type cells. However, no qualitative differences in phalloidin labeling between wild-type and mutant cells could be detected in fixed parallel samples; the cell cortex of both control and cells was decorated with actin, as were phagocytic cups and freshly ingested phagosomes (Fig. (Fig.6).6).

Figure 4
G protein β subunit mutants are impaired in yeast particle uptake. Phagocytosis of TRITC-labeled heat-killed yeast particles by AX2 (closed circle) or LW6 (open circle) was measured by incubating cells with a fivefold excess of particles ...
Figure 5
Dynamics of yeast particle uptake by wild type and β subunit–null cells. Serial light microscopy images of AX2 or LW6 cells incubated on a solid substratum with yeast particles. A total of 106 WT (AX2) or (LW6) cells ...
Table II
Quantitation of Phagocytic Cup and Phagosome Formation in Wild-Type and gβ Cells Incubated with Yeast Particles
Figure 6
Actin localization in wild-type and β subunit–null cells during phagocytosis of yeast particles. WT (AX2), rescue (LW20) and (LW6) cells incubated with TRITC-labeled yeast particles on glass coverslip. Phase-contrast ...

To follow the dynamics of actin assembly during phagocytosis in individual cells, actin was tagged with GFP (Westphal et al., 1997). Serial image analysis of GFP–actin-producing cells showed that cells, similarly to the other cell lines (AX2, AX3, and LW20), formed leading edges, in which actin was enriched, and often switched from one front to another, to which actin was newly recruited (Fig. (Fig.7).7). Thus, β subunit–null cells are not defective in rapid, spontaneous accumulation of actin at leading edges or other sites of the membrane. We monitored several events of cell–yeast particle adhesion to determine whether adhesion resulted in local actin recruitment and whether the local actin meshwork was converted into a phagocytic cup with the same probabilities in wild-type and cells. As shown in Table III, actin was found to accumulate at sites of cell–yeast particle adhesion in ~50% of the cases in both wild-type and cells. These data are consistent with the hypothesis that particle binding does not automatically trigger actin recruitment, though the low number of cases observed might have obscured differences between wild-type and mutant cells. However, independently of whether or not binding stimulates actin recruitment, the ratio of particle engulfment versus events in which actin was enriched at sites of cell–yeast adhesion was found to be significantly reduced in the mutant (Table III). In most cases no phagocytic cup, and in a few cases only a half cup was formed, followed by actin dissociation from the adhesion site after 30–90 s (Fig. (Fig.7,7, D and E). It is worth mentioning that this was usually a sufficient time interval for successful phagosome formation, both in the wild-type and in the mutant. Fig. Fig.77 E further shows that particles can attach to the surface of mutant cells for a much longer time period. Thus, cells seem to be impaired in reshaping the actin cytoskeleton at adhesion sites into a phagocytic cup and eventually a phagosome. We never observed, neither in wild-type nor in the mutant, successful uptake in the absence of locally enriched actin.

Table III
Actin Assembly and Phagocytosis in WT (AX2) and LW6 Transfected with GFP–Actin

Downstream Transduction Pathways Regulating Phagocytosis

In a preliminary attempt to dissect the cascade of signal transduction that leads from the G protein to phagocytic uptake, we tested the effects of several inhibitors on phagocytosis. Most of the drugs interfere with main effectors linked to G protein, such as PKA, PLC, and PKC, or with influx or intracellular mobilization of Ca2+ ions. Table TableIVIV summarizes the results obtained with these drugs as well as phospholipase A2 (PLA2), tyrosine kinase, and PI3/PI4 kinase inhibitors. Phagocytosis was strongly affected by PLC inhibitors, such as U73122 and manoalide, and by the intracellular Ca2+ chelator BAPTA-AM. Interestingly, U73122 interferes with G protein–dependent activation of PLC (Smith et al., 1990) and inhibits IP3-induced mobilization of Ca2+ (Willems et al., 1994; Schaloske et al., 1995). Manoalide inhibits primarily PLA2, but several other PLA2 inhibitors, active in Dictyostelium (Schaloske and Malchow, 1997), were ineffective, suggesting that PLC is the target of the observed inhibitory effect. Mobilization of intracellular Ca2+, but not Ca2+ influx, seems to also be required for efficient phagocytosis, not however via activation of PKC. Neither genistein nor wortmannin or LY294002 affected phagocytosis, which question the involvement of protein tyrosine or PI3 kinases in the process. Consistent with results obtained with the PKA inhibitors, mutants defective in activation of adenylyl cyclase or PKA, or mutants expressing constitutively PKA, showed normal rates of phagocytosis (not shown). It is worth mentioning that none of the drugs at the maximal concentration tested interfered with random cell motility or with stream formation during aggregation (data not shown). In contrast, inhibitors of phosphotyrosine phosphatases, such as phenylarsine oxide or benzylphosphonic acid-(AM)2, inhibited phagocytosis at concentrations that correlated with rounding up of the cells (data not shown).

Table IV
Summary of Drug Effects on Phagocytosis

Discussion

G Protein Controls an Early Step in Phagocytic Uptake

Deletion of the unique G protein β subunit in Dictyostelium generates a cell that lacks functional heterotrimeric G proteins. This leads to defects in multiple chemoattractant-induced responses, and, as reported here, in phagocytosis. The β subunit mutation affects an early step of the phagocytic uptake, as evidenced by the strongly reduced efficiency of individual cells in forming phagocytic cups and phagosomes around yeast particles, and by the finding that the initial rate of phagocytosis in shaken suspension is reduced. These results indicate an involvement of the heterotrimeric G protein in particle uptake, which is distinct from its potential role in intracellular phagosome–endosome fusion suggested for macrophages (Desjardins et al., 1994; Béron et al., 1995). The phenotype of the mutant differs also in many respects from Dictyostelium phagocytosis mutants previously described by us and others. Mutants defective in particle adhesion (Vogel et al., 1980; Ceccarelli and Bozzaro, 1992; Cohen et al., 1994) or talin-null mutants (Niewöhner et al., 1997) fail to phagocytose in shaken cultures but do so on bacterial lawns. In contrast, cells are impaired both under shaking conditions and on a solid substratum. Unlike phagocytosis mutants with defects in F-actin cross-linking proteins (Rivero et al., 1996; Cox et al., 1996), the mutant does not display their pleiotropic defects in cell–substratum adhesion, locomotion, or cytokinesis. This also distinguishes the β subunit deficiency from elimination of coronin, which has been suggested to lay downstream of heterotrimeric G protein and to act as an integrator of incoming signals to the cytoskeleton (Gerisch et al., 1995; Maniak et al., 1995).

G Protein Regulates the Actin Cytoskeleton during Phagocytosis

We have failed to detect differences between wild-type and cells in cell adhesion to a substrate surface, in motility or in spontaneous actin accumulation to leading edges or other sites of the membrane. This indicates that rapid reorganization of the actin cytoskeleton is not impaired in β subunit–null cells.

The cells, and to a varying degree the β subunit point mutants, fail to undergo rapid, transient actin assembly upon stimulation with cAMP or bacterial chemoattractants. This impaired actin response is correlated with the phagocytosis defect, suggesting that the G protein is involved in regulating the actin cytoskeleton during phagocytosis. The results with GFP-actin are interesting, in this context, with respect to three questions: (a) they show that spontaneous actin accumulation at leading edges or other membrane extensions is not defective in the mutant, and this is consistent with the absence of a general defect in adhesion to substrate, fluid-phase endocytosis, or cytokinesis; (b) they further show that cell contact with a yeast particle does not automatically trigger local actin assembly, neither in wild-type nor in the mutant. Actin accumulation occurs only in ~50% of the adhesion events with no differences between wild-type and mutant; and (c) they finally show that the cells, even when actin is enriched at cell– yeast adhesion sites, are strongly inhibited in their ability to reshape the actin meshwork into a phagocytic cup, and eventually a phagosome.

We thus propose that a G protein–linked process regulates an appropriate actin assembly beneath the plasma membrane leading to phagocytic cup and phagosome formation. Some evidence suggests that phagosome formation requires a different cytoskeletal organization as, for example, cell spreading (Cannon and Swanson, 1992). G protein–linked signal transduction may be required for this reorganization as occurs for actin assembly in chemotactically-induced pseudopods.

Cross-Talk between Chemotactic and Phagocytic Stimuli

There are multiple chemoattractant receptors and Gα subunits in Dictyostelium that are responsible for processing a variety of chemotactic stimuli (Parent and Devreotes, 1996). Double deletion of the chemoattractant receptors, cAR1 and cAR3, or of the α subunit Gα2 linked to these receptors, blocked responses to the chemoattractant cAMP, and deletion of the Gα4 subunit blocked responses to folic acid, but none of these mutations eliminated the chemotactic responses to bacteria-conditioned medium nor did they affect phagocytosis (data not shown). We have also not observed smaller plaques that would suggest a defect in phagocytosis in single null mutants of any of the α subunits (Gα6 has not been examined), nor did we find reduced phagocytosis rates in Gα2 and Gα4 null mutants. It is possible that the α subunits act redundantly in transducing these responses, which would make it difficult to recognize potential receptors required for phagocytosis.

Nevertheless, our results indicate that regulation of the actin cytoskeleton is a common feature in processing chemotactic and phagocytic stimuli, which are both transduced by the G protein β subunit. This is consistent with the finding that phagocytic and chemotactic stimuli compete in recruiting coronin and actin-binding proteins (Maniak et al.,1995). In contrast to chemotaxis, however, which is completely blocked in cells, phagocytosis is only partially inhibited in these cells. This may suggest either the existence of a G protein–independent pathway mediating phagocytosis or that the G protein acts at different levels in regulating the actin cytoskeleton in chemotaxis or phagocytosis.

Chemotaxis and phagocytosis in Dictyostelium exhibit a major distinct feature that also points to a different regulation of actin polymerization, or of cytoskeletal reorganization, by the β subunit. Local chemotactic stimuli trigger pseudopod formation quite automatically and lead to chemotactic locomotion, as long as the stimulus persists. In contrast, cell contact with a yeast particle does not always induce local membrane extensions to form a phagocytic cup. In addition, despite prolonged attachment of a yeast particle to the cell surface, or even formation of a phagocytic cup, the phagocytic process remains reversible, as shown by Maniak et al. (1995), who have provided evidence in support of a zipper mechanism (Swanson and Baer, 1995) for phagocytosis in Dictyostelium. The present results further confirm these observations. It is, however, possible that yeast particle uptake might differ in some important aspects from bacterial uptake, thus some caution is required in extending these conclusions to phagocytosis of bacteria.

The preliminary finding that both the intracellular Ca2+ chelator BAPTA-AM and U73122, a specific inhibitor of receptor-stimulated PLC in neutrophils (Smith et al., 1990) blocked phagocytosis suggests a role for IP3 and Ca2+ in this process, and raises the possibility that the defect in the β subunit–null cells could be linked to the inability to activate PLC. To confirm this hypothesis it will be necessary to determine which step in phagocytosis is blocked upon cell treatment with these drugs. We cannot exclude that these inhibitors affect the phagocytosis rate indirectly, by interfering with intracellular processes, such as phagosome–endosome fusion or receptor recycling, whereas we have shown that the β subunit–null cells are blocked in actin reorganization in the phagocytic cup. Interestingly, the same inhibitors did not significantly influence chemotactic motility during aggregation (data not shown), suggesting that downstream pathways leading to phagocytosis and chemotaxis might be partially different.

Activation Mechanisms of the Gβ Subunit

A G protein–dependent step in particle engulfment raises a question: what are the signals for G protein–linked actin reorganization during phagocytic cup formation? The correlation found between chemoattractant-stimulated F-actin formation and phagocytosis points to a common role for the G protein in regulating the actin cytoskeleton in chemotaxis and phagocytosis, but is no evidence that chemotactic stimuli are the triggering signals for phagocytosis. With regard to latex beads, whose uptake is decreased in cells and rescued by expression of the β subunit, the possibility of chemoattractants that may be released from bacteria is excluded. The impaired uptake of heat-killed yeast particles by cells also makes this hypothesis unlikely.

There are in principle three possibilities: (a) autocrine chemokines may exist in Dictyostelium, which are secreted upon particle binding and stimulate G protein–mediated actin reorganization; (b) the G protein, or its β subunit, could be activated in response to particle binding to yet unknown receptors; and (c) the β subunit might have an integrator function in intracellular propagation of signals arising from the site of initial particle attachment, and this function might be independent of any interactions of G proteins with specific cell-surface receptors. Activation could occur through a clustering effect resulting, for example, from the local geometry of adhesion between cell and particle, as proposed for syk tyrosine kinase involvement in phagocytosis (Greenberg et al., 1996). The correlation found between phagocytosis and chemoattractant-stimulated actin assembly, as well as the mutual competition between phagocytic cups and leading edges described by Maniak et al. (1995), support the notion that the β subunit of the heterotrimeric G protein converts signals originating from different processes into activities of the cytoskeleton.

Acknowledgments

We thank A. Noegel (Institute of Biochemistry, University of Cologne, Cologne, Germany) for anti-actin mAb, R. Albrecht and J. Murphy (both from Max Planck Institute for Biochemistry, Martinsried, Germany) for help with image processing, and S. Zigmond (University of Pennsylvania, Philadelphia, PA), S. Van Es, and N. Zhang (both from Johns Hopkins School of Medicine, Baltimore, MD) for stimulating discussions.

Abbreviations used in this paper

Gβ-null mutants
GFP–actin
green fluorescent protein–actin fusion protein
PLA2
phospholipase A2
PLC
phospholipase C
PKA
protein kinase A
PKC
protein kinase C
PI3 kinase
phosphoinositide 3-kinase
PI4 kinase
phosphoinositide 4-kinase

Footnotes

This paper was supported by grants of the European Union and Ministero Universita E Ricerca Scientifica to S. Bozzaro (ERBCHRXCT930250), the National Institutes of Health to P.N. Devreotes (GM 28007), and Deutsche Forschungsgeheinschaft to G. Gerisch (SFB266/C6).

Address all correspondence to Salvatore Bozzaro, Dip. Scienze Cliniche e Biologiche, Università di Torino, Ospedale S. Luigi, 10043 Orbassano (TO), Italy. Tel.: (39) 11-9038661. Fax: (39) 11-9038639. E-mail: sbozzaro @polito.it

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