• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jexpmedHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Exp Med. Feb 28, 1925; 41(3): 399–411.
PMCID: PMC2130947

THE RELATIVE REACTION WITHIN LIVING MAMMALIAN-TISSUES

II. ON THE MOBILIZATION OF ACID MATERIAL WITHIN CELLS, AND THE REACTION AS INFLUENCED BY THE CELL STATE.

Abstract

The acidity of the macrophage granules in which litmus comes to be stored during life is considerable. It has proved possible to stain these granules in vivo with some of the phthalein indicators and the results, had they been obtained under controlled conditions, would indicate a pH of 3.0 or less. The amount of acid material which may accumulate within the cells of animals stained with litmus is great, sufficient in the case of the elements of a peritoneal exudate for the acid reaction to prevail when they are gathered together and crushed. The material is derived, not from the dye, but from living elements responding characteristically to a stimulus far from unique. Such responses may well play a rôle in normal physiological activities and in the cellular defense against microorganisms. Vital staining with litmus demonstrates anew that the intracellular reaction during life is independent of that of the body fluids. By means of color changes in the stored indicator one can distinguish sick as well as dead cells of certain sorts and follow their distribution and fate within the organism. There are data to suggest that with the aid of the indicator the normal period of survival of certain elements at least can be determined. By the indicator method, of which the foregoing observations afford a crude illustration, much should be learnt in the future about body processes. The present paper is the second of a series upon the theme.

Full Text

The Full Text of this article is available as a PDF (652K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Rous P, Jones FS. THE PROTECTION OF PATHOGENIC MICROORGANISMS BY LIVING TISSUE CELLS. J Exp Med. 1916 May 1;23(5):601–612. [PMC free article] [PubMed]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...