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J Bacteriol. 1987 Aug; 169(8): 3853–3856.
PMCID: PMC212482

Decreased chromate uptake in Pseudomonas fluorescens carrying a chromate resistance plasmid.


CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more resistant to CrO4(2-) than those grown with 0.15 mM djenkolic acid, which derepressed the 35SrO4(2-) uptake system. Kinetics of 51CrO4(2-) uptake by P. fluorescens with and without the plasmid showed that the Vmax for 51CrO4(2-) uptake with the resistant strain was 2.2 times less than the Vmax for the sensitive strain, whereas the Km remained constant.

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Selected References

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  • Bopp LH, Chakrabarty AM, Ehrlich HL. Chromate resistance plasmid in Pseudomonas fluorescens. J Bacteriol. 1983 Sep;155(3):1105–1109. [PMC free article] [PubMed]
  • Durham DR, Phibbs PV., Jr Fractionation and characterization of the phosphoenolpyruvate: fructose 1-phosphotransferase system from Pseudomonas aeruginosa. J Bacteriol. 1982 Feb;149(2):534–541. [PMC free article] [PubMed]
  • Lebedeva EV, Lialikova NN. Vosstanovlenie krokoita kul'turoi Pseudomonas chromatophila sp. nov. Mikrobiologiia. 1979 May-Jun;48(3):517–522. [PubMed]
  • Mobley HL, Rosen BP. Energetics of plasmid-mediated arsenate resistance in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6119–6122. [PMC free article] [PubMed]
  • Ota N, Galsworthy PR, Pardee AB. Genetics of sulfate transport by Salmonella typhimurium. J Bacteriol. 1971 Mar;105(3):1053–1062. [PMC free article] [PubMed]
  • Petrilli FL, De Flora S. Toxicity and mutagenicity of hexavalent chromium on Salmonella typhimurium. Appl Environ Microbiol. 1977 Apr;33(4):805–809. [PMC free article] [PubMed]
  • Pflugrath JW, Quiocho FA. Sulphate sequestered in the sulphate-binding protein of Salmonella typhimurium is bound solely by hydrogen bonds. Nature. 1985 Mar 21;314(6008):257–260. [PubMed]
  • Silver S, Budd K, Leahy KM, Shaw WV, Hammond D, Novick RP, Willsky GR, Malamy MH, Rosenberg H. Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (III) in escherichia coli and Staphylococcus aureus. J Bacteriol. 1981 Jun;146(3):983–996. [PMC free article] [PubMed]
  • Silver S, Misra TK. Bacterial transformations of and resistances to heavy metals. Basic Life Sci. 1984;28:23–46. [PubMed]
  • Tynecka Z, Gos Z, Zajac J. Reduced cadmium transport determined by a resistance plasmid in Staphylococcus aureus. J Bacteriol. 1981 Aug;147(2):305–312. [PMC free article] [PubMed]
  • Tynecka Z, Gos Z, Zajac J. Energy-dependent efflux of cadmium coded by a plasmid resistance determinant in Staphylococcus aureus. J Bacteriol. 1981 Aug;147(2):313–319. [PMC free article] [PubMed]

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