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J Bacteriol. Apr 1987; 169(4): 1670–1677.
PMCID: PMC211998

Cyst production and brown pigment formation in aging cultures of Azospirillum brasilense ATCC 29145.

Abstract

Encystation in Azospirillum brasilense ATCC 29145 was observed by using routine laboratory staining and phase-contrast and electron microscopy. Encystment occurred in liquid and in solid or semisolid media containing fructose (8 mM) and KNO3 (0.5 mM). The encysted forms consisted of a central body filled with poly-beta-hydroxybutyric acid granules, an electron-transparent intinelike region, and a thick outer layer. Enlarged giant encysted forms with multiple central bodies were also observed during the germination of a desiccated brown colony. Morphogenetically different forms in an aging culture could be resolved by sucrose density gradient centrifugation. The dense encysted forms along with numerous granules in a fibrillar network pelleted at 70% sucrose, while empty saclike envelopes along with vegetative cells and coccoid bodies pelleted at 55% sucrose. Different media induced various degrees of pigmentation in A. brasilense ATCC 29145 after aging. The pigment possessed several of the properties reported for microbial melanins, including insolubility in water and organic solvents, solubility in cold and hot alkali, and bleaching in hydrogen peroxide. The UV absorption maxima of the alkali extract were at 280 and 310 nm. Electron micrographs of the brown pigment showed that it occurred as aggregated granules surrounding the encysting cells as well as being excreted into the medium in an aging culture. It is concluded that A. brasilense ATCC 29145 produces compounds that form a brown pigment similar to melanin and are expressed under the influence of certain cultural conditions conducive for encystment.

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  • Ballesta JP, Alexander M. Resistance of Zygorhynchus species to lysis. J Bacteriol. 1971 Jun;106(3):938–945. [PMC free article] [PubMed]
  • Beaman BL, Jackson LE, Shankel DM. Formation of multiple central bodies in giant cysts of Azotobacter vinelandii. J Bacteriol. 1968 Jul;96(1):266–269. [PMC free article] [PubMed]
  • Berg RH, Tyler ME, Novick NJ, Vasil V, Vasil IK. Biology of azospirillum-sugarcane association: enhancement of nitrogenase activity. Appl Environ Microbiol. 1980 Mar;39(3):642–649. [PMC free article] [PubMed]
  • Bloomfield BJ, Alexander M. Melanins and resistance of fungi to lysis. J Bacteriol. 1967 Apr;93(4):1276–1280. [PMC free article] [PubMed]
  • Cagle GD, Vela GR. Giant cysts and cysts with multiple central bodies in Azotobacter vinelandii. J Bacteriol. 1971 Jul;107(1):315–319. [PMC free article] [PubMed]
  • Cohen E, Okon Y, Kigel J, Nur I, Henis Y. Increase in Dry Weight and Total Nitrogen Content in Zea mays and Setaria italica Associated with Nitrogen-fixing Azospirillum spp. Plant Physiol. 1980 Oct;66(4):746–749. [PMC free article] [PubMed]
  • Das KC, Abramson MB, Katzman R. Neuronal pigments: spectroscopic characterization of human brain melanin. J Neurochem. 1978 Mar;30(3):601–605. [PubMed]
  • Delafield FP, Doudoroff M, Palleroni NJ, Lusty CJ, Contopoulos R. Decomposition of poly-beta-hydroxybutyrate by pseudomonads. J Bacteriol. 1965 Nov;90(5):1455–1466. [PMC free article] [PubMed]
  • Eskew DL, Focht DD, Ting IP. Nitrogen fixation, denitrification, and pleomorphic growth in a highly pigmented Spirillum lipoferum. Appl Environ Microbiol. 1977 Nov;34(5):582–585. [PMC free article] [PubMed]
  • Hitchins VM, Sadoff HL. Morphogenesis of cysts in Azotobacter vinelandii. J Bacteriol. 1970 Oct;104(1):492–498. [PMC free article] [PubMed]
  • Ivins BE, Holmes RK. Isolation and characterization of melanin-producing (mel) mutants of Vibrio cholerae. Infect Immun. 1980 Mar;27(3):721–729. [PMC free article] [PubMed]
  • Kuo MJ, Alexander M. Inhibition of the lysis of fungi by melanins. J Bacteriol. 1967 Sep;94(3):624–629. [PMC free article] [PubMed]
  • Kwon-Chung KJ, Rhodes JC. Encapsulation and melanin formation as indicators of virulence in Cryptococcus neoformans. Infect Immun. 1986 Jan;51(1):218–223. [PMC free article] [PubMed]
  • Ogunnariwo J, Hamilton-Miller JM. Brown- and red-pigmented Pseudomonas aeruginosa: differentiation between melanin and pyorubrin. J Med Microbiol. 1975 Feb;8(1):199–203. [PubMed]
  • O'Hara GW, Davey MR, Lucas JA. Effect of inoculation of Zea mays with Azospirillum brasilense strains under temperate conditions. Can J Microbiol. 1981 Sep;27(9):871–877. [PubMed]
  • Pope LM, Wyss O. Outer layers of the Azotobacter vinelandii cyst. J Bacteriol. 1970 Apr;102(1):234–239. [PMC free article] [PubMed]
  • Prabhakaran K, Harris EB, Kirchheimer WF. The nature of the phenolase enzyme in Mycobacterium leprae: structure-activity relationships of substrates and comparison with other copper proteins and enzymes. Microbios. 1972;5(20):273–281. [PubMed]
  • Sadasivan L, Neyra CA. Flocculation in Azospirillum brasilense and Azospirillum lipoferum: exopolysaccharides and cyst formation. J Bacteriol. 1985 Aug;163(2):716–723. [PMC free article] [PubMed]
  • Sadoff HL. Comparative aspects of morphogenesis in three prokaryotic genera. Annu Rev Microbiol. 1973;27:133–153. [PubMed]
  • Sadoff HL. Encystment and germination in Azotobacter vinelandii. Bacteriol Rev. 1975 Dec;39(4):516–539. [PMC free article] [PubMed]
  • Sen M, Sen SP. Interspecific transformation in Azotobacter. J Gen Microbiol. 1965 Oct;41(1):1–6. [PubMed]
  • Smith RL, Bouton JH, Schank SC, Quesenberry KH, Tyler ME, Milam JR, Gaskins MH, Littell RC. Nitrogen Fixation in Grasses Inoculated with Spirillum lipoferum. Science. 1976 Sep 10;193(4257):1003–1005. [PubMed]
  • Socolofsky MD, Wyss O. CYSTS OF AZOTOBACTER. J Bacteriol. 1961 Jun;81(6):946–954. [PMC free article] [PubMed]
  • SUSSMAN AS, LINGAPPA Y, BERNSTEIN IA. EFFECT OF LIGHT AND MEDIA UPON GROWTH AND MELANIN FORMATION IN CLADOSPORIUM MANSONI. Mycopathol Mycol Appl. 1963 Oct 30;20:307–314. [PubMed]
  • Umali-Garcia M, Hubbell DH, Gaskins MH, Dazzo FB. Association of azospirillum with grass roots. Appl Environ Microbiol. 1980 Jan;39(1):219–226. [PMC free article] [PubMed]
  • van Berkum P, Bohlool BB. Evaluation of nitrogen fixation by bacteria in association with roots of tropical grasses. Microbiol Rev. 1980 Sep;44(3):491–517. [PMC free article] [PubMed]
  • Vela GR. Survival of Azotobacter in dry soil. Appl Microbiol. 1974 Jul;28(1):77–79. [PMC free article] [PubMed]
  • VELA GR, WYSS O. IMPROVED STAIN FOR VISUALIZATION OF AZOTOBACTER ENCYSTMENT. J Bacteriol. 1964 Feb;87:476–477. [PMC free article] [PubMed]
  • Wheeler MH, Tolmsoff WJ, Bell AA, Mollenhauer HH. Ultrastructural and chemical distinction of melanins formed by Verticillium dahliae from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. Can J Microbiol. 1978 Mar;24(3):289–297. [PubMed]
  • Wheeler MH, Tolmsoff WJ, Meola S. Ultrastructure of melanin formation in Verticillium dahliae with (+)-scytalone as a biosynthetic intermediate. Can J Microbiol. 1976 May;22(5):702–711. [PubMed]

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