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J Cell Biol. Jan 2, 1993; 120(2): 371–383.
PMCID: PMC2119528

CHIP28 water channels are localized in constitutively water-permeable segments of the nephron

Abstract

The sites of water transport along the nephron are well characterized, but the molecular basis of renal water transport remains poorly understood. CHIP28 is a 28-kD integral protein which was proposed to mediate transmembrane water movement in red cells and kidney (Preston, G. M., T. P. Carroll, W. B. Guggino, and P. Agre. 1992. Science [Wash. DC]. 256:385-387). To determine whether CHIP28 could account for renal epithelial water transport, we used specific polyclonal antibodies to quantitate and localize CHIP28 at cellular and subcellular levels in rat kidney using light and electron microscopy. CHIP28 comprised 3.8% of isolated proximal tubule brush border protein. Except for the first few cells of the S1 segment, CHIP28 was immunolocalized throughout the convoluted and straight proximal tubules where it was observed in the microvilli of the apical brush border and in basolateral membranes. Very little CHIP28 was detected in endocytic vesicles or other intracellular structures in proximal tubules. Uninterrupted, heavy immunostaining of CHIP28 was also observed over both apical and basolateral membranes of descending thin limbs, including both short and long loops of Henle. These nephron sites have constitutively high osmotic water permeabilities. CHIP28 was not detected in ascending thin limbs, thick ascending limbs, or distal tubules, which are highly impermeable to water. Moreover, CHIP28 was not detected in collecting duct epithelia, where water permeability is regulated by antidiuretic hormone. These determinations of abundance and structural organization provide evidence that the CHIP28 water channel is the predominant pathway for constitutive transepithelial water transport in the proximal tubule and descending limb of Henle's loop.

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Selected References

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  • Agre P, Saboori AM, Asimos A, Smith BL. Purification and partial characterization of the Mr 30,000 integral membrane protein associated with the erythrocyte Rh(D) antigen. J Biol Chem. 1987 Dec 25;262(36):17497–17503. [PubMed]
  • Bennett V. Proteins involved in membrane--cytoskeleton association in human erythrocytes: spectrin, ankyrin, and band 3. Methods Enzymol. 1983;96:313–324. [PubMed]
  • Brown D. Membrane recycling and epithelial cell function. Am J Physiol. 1989 Jan;256(1 Pt 2):F1–12. [PubMed]
  • Brown D. Structural-functional features of antidiuretic hormone-induced water transport in the collecting duct. Semin Nephrol. 1991 Jul;11(4):478–501. [PubMed]
  • Burg MB, Green N. Function of the thick ascending limb of Henle's loop. Am J Physiol. 1973 Mar;224(3):659–668. [PubMed]
  • Chou CL, Sands JM, Nonoguchi H, Knepper MA. Urea gradient-associated fluid absorption with sigma urea = 1 in rat terminal collecting duct. Am J Physiol. 1990 May;258(5 Pt 2):F1173–F1180. [PubMed]
  • Chou CL, Knepper MA. In vitro perfusion of chinchilla thin limb segments: segmentation and osmotic water permeability. Am J Physiol. 1992 Sep;263(3 Pt 2):F417–F426. [PubMed]
  • Davis JQ, Bennett V. Brain ankyrin. Purification of a 72,000 Mr spectrin-binding domain. J Biol Chem. 1984 Feb 10;259(3):1874–1881. [PubMed]
  • Denker BM, Smith BL, Kuhajda FP, Agre P. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J Biol Chem. 1988 Oct 25;263(30):15634–15642. [PubMed]
  • Gorin MB, Yancey SB, Cline J, Revel JP, Horwitz J. The major intrinsic protein (MIP) of the bovine lens fiber membrane: characterization and structure based on cDNA cloning. Cell. 1984 Nov;39(1):49–59. [PubMed]
  • Green R, Giebisch G. Reflection coefficients and water permeability in rat proximal tubule. Am J Physiol. 1989 Oct;257(4 Pt 2):F658–F668. [PubMed]
  • Griffiths G, McDowall A, Back R, Dubochet J. On the preparation of cryosections for immunocytochemistry. J Ultrastruct Res. 1984 Oct;89(1):65–78. [PubMed]
  • Gross JB, Imai M, Kokko JP. A functional comparison of the cortical collecting tubule and the distal convoluted tubule. J Clin Invest. 1975 Jun;55(6):1284–1294. [PMC free article] [PubMed]
  • Handler JS. Antidiuretic hormone moves membranes. Am J Physiol. 1988 Sep;255(3 Pt 2):F375–F382. [PubMed]
  • Harris HW, Jr, Strange K, Zeidel ML. Current understanding of the cellular biology and molecular structure of the antidiuretic hormone-stimulated water transport pathway. J Clin Invest. 1991 Jul;88(1):1–8. [PMC free article] [PubMed]
  • Horster MF, Zink H. Functional differentiation of the medullary collecting tubule: influence of vasopressin. Kidney Int. 1982 Oct;22(4):360–365. [PubMed]
  • Imai M. The connecting tubule: a functional subdivision of the rabbit distal nephron segments. Kidney Int. 1979 Apr;15(4):346–356. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lankford SP, Chou CL, Terada Y, Wall SM, Wade JB, Knepper MA. Regulation of collecting duct water permeability independent of cAMP-mediated AVP response. Am J Physiol. 1991 Sep;261(3 Pt 2):F554–F566. [PubMed]
  • Murer H, Gmaj P. Transport studies in plasma membrane vesicles isolated from renal cortex. Kidney Int. 1986 Aug;30(2):171–186. [PubMed]
  • Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11110–11114. [PMC free article] [PubMed]
  • Preston GM, Carroll TP, Guggino WB, Agre P. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science. 1992 Apr 17;256(5055):385–387. [PubMed]
  • Reif MC, Troutman SL, Schafer JA. Sustained response to vasopressin in isolated rat cortical collecting tubule. Kidney Int. 1984 Nov;26(5):725–732. [PubMed]
  • Rocha AS, Kokko JP. Sodium chloride and water transport in the medullary thick ascending limb of Henle. Evidence for active chloride transport. J Clin Invest. 1973 Mar;52(3):612–623. [PMC free article] [PubMed]
  • Rodriguez-Boulan E, Nelson WJ. Morphogenesis of the polarized epithelial cell phenotype. Science. 1989 Aug 18;245(4919):718–725. [PubMed]
  • Saboori AM, Smith BL, Agre P. Polymorphism in the Mr 32,000 Rh protein purified from Rh(D)-positive and -negative erythrocytes. Proc Natl Acad Sci U S A. 1988 Jun;85(11):4042–4045. [PMC free article] [PubMed]
  • Schafer JA, Patlak CS, Troutman SL, Andreoli TE. Volume absorption in the pars recta. II. Hydraulic conductivity coefficient. Am J Physiol. 1978 Apr;234(4):F340–F348. [PubMed]
  • Smith BL, Agre P. Erythrocyte Mr 28,000 transmembrane protein exists as a multisubunit oligomer similar to channel proteins. J Biol Chem. 1991 Apr 5;266(10):6407–6415. [PubMed]
  • Tokuyasu KT. Application of cryoultramicrotomy to immunocytochemistry. J Microsc. 1986 Aug;143(Pt 2):139–149. [PubMed]
  • Verkman AS. Mechanisms and regulation of water permeability in renal epithelia. Am J Physiol. 1989 Nov;257(5 Pt 1):C837–C850. [PubMed]
  • Zeidel ML, Ambudkar SV, Smith BL, Agre P. Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein. Biochemistry. 1992 Aug 25;31(33):7436–7440. [PubMed]

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