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J Cell Biol. Jan 1, 1993; 120(1): 163–173.
PMCID: PMC2119478

Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility

Abstract

Coronin is an actin-binding protein in Dictyostelium discoideum that is enriched at the leading edge of the cells and in projections of the cell surface called crowns. The polypeptide sequence of coronin is distinguished by its similarities to the beta-subunits of trimeric G proteins (E. L. de Hostos, B. Bradtke, F. Lottspeich, R. Guggenheim, and G. Gerisch, 1991. EMBO (Eur. Mol. Biol. Organ.) J. 10:4097-4104). To elucidate the in vivo function of coronin, null mutants have been generated by gene replacement. The mutant cells lacking coronin grow and migrate more slowly than wild-type cells. When these cor- cells grow in liquid medium they become multinucleate, indicating a role of coronin in cytokinesis. To explore this role, coronin has been localized in mitotic wild-type cells by immunofluorescence labeling. During separation of the daughter cells, coronin is strongly accumulated at their distal portions including the leading edges. This contrasts with the localization of myosin II in the cleavage furrow and suggests that coronin functions independently of the conventional myosin in facilitating cytokinesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • André E, Brink M, Gerisch G, Isenberg G, Noegel A, Schleicher M, Segall JE, Wallraff E. A Dictyostelium mutant deficient in severin, an F-actin fragmenting protein, shows normal motility and chemotaxis. J Cell Biol. 1989 Mar;108(3):985–995. [PMC free article] [PubMed]
  • Ashworth JM, Watts DJ. Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture. Biochem J. 1970 Sep;119(2):175–182. [PMC free article] [PubMed]
  • Barr FA, Leyte A, Huttner WB. Trimeric G proteins and vesicle formation. Trends Cell Biol. 1992 Apr;2(4):91–94. [PubMed]
  • Berlot CH, Spudich JA, Devreotes PN. Chemoattractant-elicited increases in myosin phosphorylation in Dictyostelium. Cell. 1985 Nov;43(1):307–314. [PubMed]
  • Bomblies L, Biegelmann E, Döring V, Gerisch G, Krafft-Czepa H, Noegel AA, Schleicher M, Humbel BM. Membrane-enclosed crystals in Dictyostelium discoideum cells, consisting of developmentally regulated proteins with sequence similarities to known esterases. J Cell Biol. 1990 Mar;110(3):669–679. [PMC free article] [PubMed]
  • Brink M, Gerisch G, Isenberg G, Noegel AA, Segall JE, Wallraff E, Schleicher M. A Dictyostelium mutant lacking an F-actin cross-linking protein, the 120-kD gelation factor. J Cell Biol. 1990 Oct;111(4):1477–1489. [PMC free article] [PubMed]
  • Burgoyne RD. Trimeric G proteins in Golgi transport. Trends Biochem Sci. 1992 Mar;17(3):87–88. [PubMed]
  • Choi WJ, Clark MW, Chen JX, Jong AY. The CDC4 gene product is associated with the yeast nuclear skeleton. Biochem Biophys Res Commun. 1990 Nov 15;172(3):1324–1330. [PubMed]
  • Claviez M, Pagh K, Maruta H, Baltes W, Fisher P, Gerisch G. Electron microscopic mapping of monoclonal antibodies on the tail region of Dictyostelium myosin. EMBO J. 1982;1(8):1017–1022. [PMC free article] [PubMed]
  • Cox D, Condeelis J, Wessels D, Soll D, Kern H, Knecht DA. Targeted disruption of the ABP-120 gene leads to cells with altered motility. J Cell Biol. 1992 Feb;116(4):943–955. [PMC free article] [PubMed]
  • Cunningham CC, Stossel TP, Kwiatkowski DJ. Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin. Science. 1991 Mar 8;251(4998):1233–1236. [PubMed]
  • Cunningham CC, Gorlin JB, Kwiatkowski DJ, Hartwig JH, Janmey PA, Byers HR, Stossel TP. Actin-binding protein requirement for cortical stability and efficient locomotion. Science. 1992 Jan 17;255(5042):325–327. [PubMed]
  • de Hostos EL, Bradtke B, Lottspeich F, Guggenheim R, Gerisch G. Coronin, an actin binding protein of Dictyostelium discoideum localized to cell surface projections, has sequence similarities to G protein beta subunits. EMBO J. 1991 Dec;10(13):4097–4104. [PMC free article] [PubMed]
  • De Lozanne A, Spudich JA. Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. Science. 1987 May 29;236(4805):1086–1091. [PubMed]
  • Devreotes PN, Zigmond SH. Chemotaxis in eukaryotic cells: a focus on leukocytes and Dictyostelium. Annu Rev Cell Biol. 1988;4:649–686. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Fisher PR, Smith E, Williams KL. An extracellular chemical signal controlling phototactic behavior by D. discoideum slugs. Cell. 1981 Mar;23(3):799–807. [PubMed]
  • Fisher PR, Grant WN, Dohrmann U, Williams KL. Spontaneous turning behaviour by Dictyostelium discoideum slugs. J Cell Sci. 1983 Jul;62:161–170. [PubMed]
  • Fisher PR, Merkl R, Gerisch G. Quantitative analysis of cell motility and chemotaxis in Dictyostelium discoideum by using an image processing system and a novel chemotaxis chamber providing stationary chemical gradients. J Cell Biol. 1989 Mar;108(3):973–984. [PMC free article] [PubMed]
  • Fukui Y, Inoué S. Cell division in Dictyostelium with special emphasis on actomyosin organization in cytokinesis. Cell Motil Cytoskeleton. 1991;18(1):41–54. [PubMed]
  • Fukui Y, Yumura S, Yumura TK. Agar-overlay immunofluorescence: high-resolution studies of cytoskeletal components and their changes during chemotaxis. Methods Cell Biol. 1987;28:347–356. [PubMed]
  • Fukui Y, De Lozanne A, Spudich JA. Structure and function of the cytoskeleton of a Dictyostelium myosin-defective mutant. J Cell Biol. 1990 Feb;110(2):367–378. [PMC free article] [PubMed]
  • Gerisch G, Noegel AA, Schleicher M. Genetic alteration of proteins in actin-based motility systems. Annu Rev Physiol. 1991;53:607–628. [PubMed]
  • Jay PY, Elson EL. Surface particle transport mechanism independent of myosin II in Dictyostelium. Nature. 1992 Apr 2;356(6368):438–440. [PubMed]
  • Jung G, Hammer JA., 3rd Generation and characterization of Dictyostelium cells deficient in a myosin I heavy chain isoform. J Cell Biol. 1990 Jun;110(6):1955–1964. [PMC free article] [PubMed]
  • Knecht DA, Loomis WF. Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science. 1987 May 29;236(4805):1081–1086. [PubMed]
  • Knecht DA, Cohen SM, Loomis WF, Lodish HF. Developmental regulation of Dictyostelium discoideum actin gene fusions carried on low-copy and high-copy transformation vectors. Mol Cell Biol. 1986 Nov;6(11):3973–3983. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Manstein DJ, Titus MA, De Lozanne A, Spudich JA. Gene replacement in Dictyostelium: generation of myosin null mutants. EMBO J. 1989 Mar;8(3):923–932. [PMC free article] [PubMed]
  • Mehdy MC, Ratner D, Firtel RA. Induction and modulation of cell-type-specific gene expression in Dictyostelium. Cell. 1983 Mar;32(3):763–771. [PubMed]
  • Pollard TD, Cooper JA. Actin and actin-binding proteins. A critical evaluation of mechanisms and functions. Annu Rev Biochem. 1986;55:987–1035. [PubMed]
  • Satterwhite LL, Pollard TD. Cytokinesis. Curr Opin Cell Biol. 1992 Feb;4(1):43–52. [PubMed]
  • Schleicher M, Noegel AA. Dynamics of the Dictyostelium cytoskeleton during chemotaxis. New Biol. 1992 May;4(5):461–472. [PubMed]
  • Segall JE, Fisher PR, Gerisch G. Selection of chemotaxis mutants of Dictyostelium discoideum. J Cell Biol. 1987 Jan;104(1):151–161. [PMC free article] [PubMed]
  • Titus MA, Warrick HM, Spudich JA. Multiple actin-based motor genes in Dictyostelium. Cell Regul. 1989 Nov;1(1):55–63. [PMC free article] [PubMed]
  • van der Voorn L, Gebbink M, Plasterk RH, Ploegh HL. Characterization of a G-protein beta-subunit gene from the nematode Caenorhabditis elegans. J Mol Biol. 1990 May 5;213(1):17–26. [PubMed]
  • Waddell DR, Duffy K, Vogel G. Cytokinesis is defective in Dictyostelium mutants with altered phagocytic recognition, adhesion, and vegetative cell cohesion properties. J Cell Biol. 1987 Nov;105(5):2293–2300. [PMC free article] [PubMed]
  • Wallraff E, Schleicher M, Modersitzki M, Rieger D, Isenberg G, Gerisch G. Selection of Dictyostelium mutants defective in cytoskeletal proteins: use of an antibody that binds to the ends of alpha-actinin rods. EMBO J. 1986 Jan;5(1):61–67. [PMC free article] [PubMed]
  • Wessels D, Soll DR. Myosin II heavy chain null mutant of Dictyostelium exhibits defective intracellular particle movement. J Cell Biol. 1990 Sep;111(3):1137–1148. [PMC free article] [PubMed]
  • Wessels D, Soll DR, Knecht D, Loomis WF, De Lozanne A, Spudich J. Cell motility and chemotaxis in Dictyostelium amebae lacking myosin heavy chain. Dev Biol. 1988 Jul;128(1):164–177. [PubMed]
  • Witke W, Nellen W, Noegel A. Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein. EMBO J. 1987 Dec 20;6(13):4143–4148. [PMC free article] [PubMed]
  • Witke W, Schleicher M, Noegel AA. Redundancy in the microfilament system: abnormal development of Dictyostelium cells lacking two F-actin cross-linking proteins. Cell. 1992 Jan 10;68(1):53–62. [PubMed]
  • Yochem J, Byers B. Structural comparison of the yeast cell division cycle gene CDC4 and a related pseudogene. J Mol Biol. 1987 May 20;195(2):233–245. [PubMed]

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