• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Mar 1987; 169(3): 1341–1344.
PMCID: PMC211943

Efficient transfection of the archaebacterium Halobacterium halobium.


We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA. Between 0.5 and 1% of spheroplasts were transfected per microgram of luminal diameter H DNA. Under our conditions, survival and regeneration of H. halobium spheroplasts were also quite efficient, suggesting that this method will be useful for introducing other DNAs into these bacteria.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (665K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Klebe RJ, Harriss JV, Sharp ZD, Douglas MG. A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast. Gene. 1983 Nov;25(2-3):333–341. [PubMed]
  • Lanyi JK, Plachy WZ, Kates M. Lipid interactions in membranes of extremely halophilic bacteria. II. Modification of the bilayer structure by squalene. Biochemistry. 1974 Nov 19;13(24):4914–4920. [PubMed]
  • Lis JT. Fractionation of DNA fragments by polyethylene glycol induced precipitation. Methods Enzymol. 1980;65(1):347–353. [PubMed]
  • Mescher MF, Strominger JL. Structural (shape-maintaining) role of the cell surface glycoprotein of Halobacterium salinarium. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2687–2691. [PMC free article] [PubMed]
  • Mevarech M, Werczberger R. Genetic transfer in Halobacterium volcanii. J Bacteriol. 1985 Apr;162(1):461–462. [PMC free article] [PubMed]
  • Schnabel H, Zillig W, Pfäffle M, Schnabel R, Michel H, Delius H. Halobacterium halobium phage øH. EMBO J. 1982;1(1):87–92. [PMC free article] [PubMed]
  • Sumper M, Herrmann G. Studies on the biosynthesis of bacterio-opsin. Demonstration of the existence of protein species structurally related to bacterio-opsin. Eur J Biochem. 1978 Aug 15;89(1):229–235. [PubMed]
  • Woese CR, Fox GE. Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088–5090. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...