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J Cell Biol. 1989 Jun 1; 108(6): 2459–2466.
PMCID: PMC2115581

Basic fibroblast growth factor in the chick embryo: immunolocalization to striated muscle cells and their precursors


The identification of acidic and basic fibroblast growth factors (FGFs) in a number of embryonic tissue extracts has implicated these growth factors in the regulation of a variety of embryonic events including angiogenesis, eye development, and muscle differentiation. Lack of information concerning the cellular distribution of the growth factor within these tissues has made it extremely difficult to assign developmental roles to FGF. We have localized bFGF in the developing chick embryo using immunohistochemical techniques and our monospecific polyclonal rabbit anti-human bFGF IgG. The spatial pattern for bFGF localization was highly specific. The anti-human bFGF antibodies recognized striated muscle cells and their precursors in 2-6-d chick embryos. Myocardium, somite myotome, and limb bud muscle all stain positively for bFGF. In addition, the anti-human bFGF antibodies localized specifically to the cell, rather than to the extracellular matrix or nucleus of myotubes. The localization of bFGF demonstrated here provides further support for the hypothesis (Clegg et al., 1987; Seed et al., 1988) that this growth factor is involved in muscle development.

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Selected References

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  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Clegg CH, Linkhart TA, Olwin BB, Hauschka SD. Growth factor control of skeletal muscle differentiation: commitment to terminal differentiation occurs in G1 phase and is repressed by fibroblast growth factor. J Cell Biol. 1987 Aug;105(2):949–956. [PMC free article] [PubMed]
  • Esch F, Baird A, Ling N, Ueno N, Hill F, Denoroy L, Klepper R, Gospodarowicz D, Böhlen P, Guillemin R. Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6507–6511. [PMC free article] [PubMed]
  • Gospodarowicz D, Cheng J, Lirette M. Bovine brain and pituitary fibroblast growth factors: comparison of their abilities to support the proliferation of human and bovine vascular endothelial cells. J Cell Biol. 1983 Dec;97(6):1677–1685. [PMC free article] [PubMed]
  • Gospodarowicz D, Massoglia S, Cheng J, Fujii DK. Effect of fibroblast growth factor and lipoproteins on the proliferation of endothelial cells derived from bovine adrenal cortex, brain cortex, and corpus luteum capillaries. J Cell Physiol. 1986 Apr;127(1):121–136. [PubMed]
  • Gospodarowicz D, Neufeld G, Schweigerer L. Molecular and biological characterization of fibroblast growth factor, an angiogenic factor which also controls the proliferation and differentiation of mesoderm and neuroectoderm derived cells. Cell Differ. 1986 Jul;19(1):1–17. [PubMed]
  • Hatten ME, Lynch M, Rydel RE, Sanchez J, Joseph-Silverstein J, Moscatelli D, Rifkin DB. In vitro neurite extension by granule neurons is dependent upon astroglial-derived fibroblast growth factor. Dev Biol. 1988 Feb;125(2):280–289. [PubMed]
  • Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. [PubMed]
  • Joseph-Silverstein J, Rifkin DB. Endothelial cell growth factors and the vessel wall. Semin Thromb Hemost. 1987 Oct;13(4):504–513. [PubMed]
  • Joseph-Silverstein J, Moscatelli D, Rifkin DB. The development of a quantitative RIA for basic fibroblast growth factor using polyclonal antibodies against the 157 amino acid form of human bFGF. The identification of bFGF in adherent elicited murine peritoneal macrophages. J Immunol Methods. 1988 Jun 13;110(2):183–192. [PubMed]
  • Kardami E, Spector D, Strohman RC. Myogenic growth factor present in skeletal muscle is purified by heparin-affinity chromatography. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8044–8047. [PMC free article] [PubMed]
  • Kardami E, Spector D, Strohman RC. Selected muscle and nerve extracts contain an activity which stimulates myoblast proliferation and which is distinct from transferrin. Dev Biol. 1985 Dec;112(2):353–358. [PubMed]
  • Kimelman D, Kirschner M. Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo. Cell. 1987 Dec 4;51(5):869–877. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lobb RR, Harper JW, Fett JW. Purification of heparin-binding growth factors. Anal Biochem. 1986 Apr;154(1):1–14. [PubMed]
  • Manasek FJ. Embryonic development of the heart. I. A light and electron microscopic study of myocardial development in the early chick embryo. J Morphol. 1968 Jul;125(3):329–365. [PubMed]
  • Mascarelli F, Raulais D, Counis MF, Courtois Y. Characterization of acidic and basic fibroblast growth factors in brain, retina and vitreous chick embryo. Biochem Biophys Res Commun. 1987 Jul 31;146(2):478–486. [PubMed]
  • Moscatelli D, Presta M, Rifkin DB. Purification of a factor from human placenta that stimulates capillary endothelial cell protease production, DNA synthesis, and migration. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2091–2095. [PMC free article] [PubMed]
  • Moscatelli D, Presta M, Joseph-Silverstein J, Rifkin DB. Both normal and tumor cells produce basic fibroblast growth factor. J Cell Physiol. 1986 Nov;129(2):273–276. [PubMed]
  • Olwin BB, Hauschka SD. Identification of the fibroblast growth factor receptor of Swiss 3T3 cells and mouse skeletal muscle myoblasts. Biochemistry. 1986 Jun 17;25(12):3487–3492. [PubMed]
  • Olwin BB, Hauschka SD. Cell surface fibroblast growth factor and epidermal growth factor receptors are permanently lost during skeletal muscle terminal differentiation in culture. J Cell Biol. 1988 Aug;107(2):761–769. [PMC free article] [PubMed]
  • Pettmann B, Weibel M, Sensenbrenner M, Labourdette G. Purification of two astroglial growth factors from bovine brain. FEBS Lett. 1985 Sep 9;189(1):102–108. [PubMed]
  • Risau W. Developing brain produces an angiogenesis factor. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3855–3859. [PMC free article] [PubMed]
  • Risau W, Ekblom P. Production of a heparin-binding angiogenesis factor by the embryonic kidney. J Cell Biol. 1986 Sep;103(3):1101–1107. [PMC free article] [PubMed]
  • Seed J, Hauschka SD. Clonal analysis of vertebrate myogenesis. VIII. Fibroblasts growth factor (FGF)-dependent and FGF-independent muscle colony types during chick wing development. Dev Biol. 1988 Jul;128(1):40–49. [PubMed]
  • Seed J, Olwin BB, Hauschka SD. Fibroblast growth factor levels in the whole embryo and limb bud during chick development. Dev Biol. 1988 Jul;128(1):50–57. [PubMed]
  • Shing Y, Folkman J, Haudenschild C, Lund D, Crum R, Klagsbrun M. Angiogenesis is stimulated by a tumor-derived endothelial cell growth factor. J Cell Biochem. 1985;29(4):275–287. [PubMed]
  • Slack JM, Darlington BG, Heath JK, Godsave SF. Mesoderm induction in early Xenopus embryos by heparin-binding growth factors. Nature. 1987 Mar 12;326(6109):197–200. [PubMed]
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. [PMC free article] [PubMed]
  • Unsicker K, Reichert-Preibsch H, Schmidt R, Pettmann B, Labourdette G, Sensenbrenner M. Astroglial and fibroblast growth factors have neurotrophic functions for cultured peripheral and central nervous system neurons. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5459–5463. [PMC free article] [PubMed]
  • Vlodavsky I, Folkman J, Sullivan R, Fridman R, Ishai-Michaeli R, Sasse J, Klagsbrun M. Endothelial cell-derived basic fibroblast growth factor: synthesis and deposition into subendothelial extracellular matrix. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2292–2296. [PMC free article] [PubMed]
  • Wilkinson DG, Peters G, Dickson C, McMahon AP. Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse. EMBO J. 1988 Mar;7(3):691–695. [PMC free article] [PubMed]

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