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J Cell Biol. 1989 May 1; 108(5): 1687–1695.
PMCID: PMC2115551

Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium


Cryptdin mRNA codes for the apparent precursor to a corticostatin/defensin-related peptide that accumulates to high levels in mouse intestinal crypt epithelium during postnatal development. The primary structure, intestinal cell distribution, and developmental appearance of cryptdin mRNA have been determined. Cryptdin mRNA is 450- 480 nucleotides long. Translation of the partial cryptdin cDNA sequence reveals a 70-amino acid open reading frame that includes 32 carboxy- terminal residues that align with those in the consensus sequence, C.CR...C....ER..G.C....CCR, which is a common feature of leukocyte defensins and lung corticostatins (Selsted, M. E., D. M. Brown, R. J. DeLange, S. S. L. Harwig, and R. I. Lehrer. 1985. J. Biol. Chem. 260:4579-4584; Zhu, Q., J. Hu, S. Mulay, F. Esch, S. Shimasaki, and S. Solomon. 1988. Proc. Natl. Acad. Sci. USA. 85:592-596). In situ hybridization of cryptdin cDNA to paraformaldehyde-fixed, frozen sections of adult jejunum and ileum showed intense and specific labeling of epithelial cells in the base of all crypts. Analysis of sections from suckling mice showed that cryptdin mRNA is detectable in 10-20% of crypts in 10-d-old mice, in approximately 80% of crypts in 16- d-old mice, and in all crypts of mice 20 d and older. During the fourth week, the sequence accumulates in crypts to the maximal adult level. Cryptdin mRNA content in adult small intestine is independent both of T cell involvement and luminal bacteria. The role of cryptdin in small bowel physiology remains to be determined: cryptdin may inhibit bacterial translocation, modulate intestinal hormone synthesis, influence hormonal sensitivity of the intestinal epithelium, or exhibit a multiplicity of related activities.

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Selected References

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  • Altmann GG, Enesco M. Cell number as a measure of distribution and renewal of epithelial cells in the small intestine of growing and adult rats. Am J Anat. 1967 Sep;121(2):319–336. [PubMed]
  • Angerer LM, Cox KH, Angerer RC. Demonstration of tissue-specific gene expression by in situ hybridization. Methods Enzymol. 1987;152:649–661. [PubMed]
  • Bach AC, 2nd, Selsted ME, Pardi A. Two-dimensional NMR studies of the antimicrobial peptide NP-5. Biochemistry. 1987 Jul 14;26(14):4389–4397. [PubMed]
  • Biggin MD, Gibson TJ, Hong GF. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963–3965. [PMC free article] [PubMed]
  • Birkenmeier EH, Gordon JI. Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2516–2520. [PMC free article] [PubMed]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Elmes ME, Stanton MR, Howells CH, Lowe GH. Relation between the mucosal flora and Paneth cell population of human jejunum and ileum. J Clin Pathol. 1984 Nov;37(11):1268–1271. [PMC free article] [PubMed]
  • Erlandsen SL, Chase DG. Paneth cell function: phagocytosis and intracellular digestion of intestinal microorganisms. I. Hexamita muris. J Ultrastruct Res. 1972 Nov;41(3):296–318. [PubMed]
  • Erlandsen SL, Parsons JA, Taylor TD. Ultrastructural immunocytochemical localization of lysozyme in the Paneth cells of man. J Histochem Cytochem. 1974 Jun;22(6):401–413. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. [PubMed]
  • Ganz T, Sherman MP, Selsted ME, Lehrer RI. Newborn rabbit alveolar macrophages are deficient in two microbicidal cationic peptides, MCP-1 and MCP-2. Am Rev Respir Dis. 1985 Oct;132(4):901–904. [PubMed]
  • Ganz T, Selsted ME, Szklarek D, Harwig SS, Daher K, Bainton DF, Lehrer RI. Defensins. Natural peptide antibiotics of human neutrophils. J Clin Invest. 1985 Oct;76(4):1427–1435. [PMC free article] [PubMed]
  • George DG, Barker WC, Hunt LT. The protein identification resource (PIR). Nucleic Acids Res. 1986 Jan 10;14(1):11–15. [PMC free article] [PubMed]
  • Guy-Grand D, Griscelli C, Vassalli P. The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978 Dec 1;148(6):1661–1677. [PMC free article] [PubMed]
  • Hammer MF, Schilling JW, Prager EM, Wilson AC. Recruitment of lysozyme as a major enzyme in the mouse gut: duplication, divergence, and regulatory evolution. J Mol Evol. 1987;24(3):272–279. [PubMed]
  • Henning SJ. Ontogeny of enzymes in the small intestine. Annu Rev Physiol. 1985;47:231–245. [PubMed]
  • Herbst JJ, Sunshine P. Postnatal development of the small intestine of the rat. Changes in mucosal morphology at weaning. Pediatr Res. 1969 Jan;3(1):27–33. [PubMed]
  • Huang WM, Gibson SJ, Facer P, Gu J, Polak JM. Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating. Histochemistry. 1983;77(2):275–279. [PubMed]
  • Klein RM, McKenzie JC. The role of cell renewal in the ontogeny of the intestine. II. Regulation of cell proliferation in adult, fetal, and neonatal intestine. J Pediatr Gastroenterol Nutr. 1983 May;2(2):204–228. [PubMed]
  • Koldovsky O, Sunshine P, Kretchmer N. Cellular migration of intestinal epithelia in suckling and weaned rats. Nature. 1966 Dec 17;212(5068):1389–1390. [PubMed]
  • Lehrer RI, Selsted ME, Szklarek D, Fleischmann J. Antibacterial activity of microbicidal cationic proteins 1 and 2, natural peptide antibiotics of rabbit lung macrophages. Infect Immun. 1983 Oct;42(1):10–14. [PMC free article] [PubMed]
  • Marasco WA, Phan SH, Krutzsch H, Showell HJ, Feltner DE, Nairn R, Becker EL, Ward PA. Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli. J Biol Chem. 1984 May 10;259(9):5430–5439. [PubMed]
  • MOOG F. Developmental adaptations of alkaline phosphatases in the small intestine. Fed Proc. 1962 Jan-Feb;21:51–56. [PubMed]
  • Ouellette AJ, Cordell B. Accumulation of abundant messenger ribonucleic acids during postnatal development of mouse small intestine. Gastroenterology. 1988 Jan;94(1):114–121. [PubMed]
  • Perry RP, La Torre J, Kelley DE, Greenberg JR. On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochim Biophys Acta. 1972 Mar 14;262(2):220–226. [PubMed]
  • Raul F, Simon P, Kedinger M, Haffen K. Intestinal enzymes activities in isolated villus and crypt cells during postnatal development of the rat. Cell Tissue Res. 1977 Jan 12;176(2):167–178. [PubMed]
  • Rave N, Crkvenjakov R, Boedtker H. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Res. 1979 Aug 10;6(11):3559–3567. [PMC free article] [PubMed]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Satoh Y, Vollrath L. Quantitative electron microscopic observations on Paneth cells of germfree and ex-germfree Wistar rats. Anat Embryol (Berl) 1986;173(3):317–322. [PubMed]
  • Satoh Y, Ishikawa K, Ono K, Vollrath L. Quantitative light microscopic observations on Paneth cells of germ-free and ex-germ-free Wistar rats. Digestion. 1986;34(2):115–121. [PubMed]
  • Selsted ME, Harwig SS. Purification, primary structure, and antimicrobial activities of a guinea pig neutrophil defensin. Infect Immun. 1987 Sep;55(9):2281–2286. [PMC free article] [PubMed]
  • Selsted ME, Brown DM, DeLange RJ, Lehrer RI. Primary structures of MCP-1 and MCP-2, natural peptide antibiotics of rabbit lung macrophages. J Biol Chem. 1983 Dec 10;258(23):14485–14489. [PubMed]
  • Selsted ME, Szklarek D, Lehrer RI. Purification and antibacterial activity of antimicrobial peptides of rabbit granulocytes. Infect Immun. 1984 Jul;45(1):150–154. [PMC free article] [PubMed]
  • Selsted ME, Brown DM, DeLange RJ, Harwig SS, Lehrer RI. Primary structures of six antimicrobial peptides of rabbit peritoneal neutrophils. J Biol Chem. 1985 Apr 25;260(8):4579–4584. [PubMed]
  • Selsted ME, Harwig SS, Ganz T, Schilling JW, Lehrer RI. Primary structures of three human neutrophil defensins. J Clin Invest. 1985 Oct;76(4):1436–1439. [PMC free article] [PubMed]
  • Staden R. Computer methods to aid the determination and analysis of DNA sequences. Biochem Soc Trans. 1984 Dec;12(6):1005–1008. [PubMed]
  • Staden R. The current status and portability of our sequence handling software. Nucleic Acids Res. 1986 Jan 10;14(1):217–231. [PMC free article] [PubMed]
  • Stanfield RL, Westbrook EM, Selsted ME. Characterization of two crystal forms of human defensin neutrophil cationic peptide 1, a naturally occurring antimicrobial peptide of leukocytes. J Biol Chem. 1988 Apr 25;263(12):5933–5935. [PubMed]
  • Thomas PS. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. [PMC free article] [PubMed]
  • Westbrook EM, Lehrer RI, Selsted ME. Characterization of two crystal forms of neutrophil cationic protein NP2, a naturally occurring broad-spectrum antimicrobial agent from leukocytes. J Mol Biol. 1984 Sep 25;178(3):783–785. [PubMed]
  • Zhu QZ, Hu J, Mulay S, Esch F, Shimasaki S, Solomon S. Isolation and structure of corticostatin peptides from rabbit fetal and adult lung. Proc Natl Acad Sci U S A. 1988 Jan;85(2):592–596. [PMC free article] [PubMed]

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