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J Cell Biol. 1987 Jul 1; 105(1): 371–379.
PMCID: PMC2114938

Cap Z(36/32), a barbed end actin-capping protein, is a component of the Z-line of skeletal muscle

Abstract

Various biological activities have been attributed to actin-capping proteins based on their in vitro effects on actin filaments. However, there is little direct evidence for their in vivo activities. In this paper, we show that Cap Z(36/32), a barbed end, actin-capping protein isolated from muscle (Casella, J. F., D. J. Maack, and S. Lin, 1986, J. Biol. Chem., 261:10915-10921) is localized to the barbed ends of actin filaments by electron microscopy and to the Z-line of chicken skeletal muscle by indirect immunofluorescence and electron microscopy. Since actin filaments associate with the Z-line at their barbed ends, these findings suggest that Cap Z(36/32) may play a role in regulating length, orienting, or attaching actin filaments to Z-discs.

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Selected References

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  • Bretscher A, Weber K. Villin is a major protein of the microvillus cytoskeleton which binds both G and F actin in a calcium-dependent manner. Cell. 1980 Jul;20(3):839–847. [PubMed]
  • Carron CP, Hwo SY, Dingus J, Benson DM, Meza I, Bryan J. A re-evaluation of cytoplasmic gelsolin localization. J Cell Biol. 1986 Jan;102(1):237–245. [PMC free article] [PubMed]
  • Casella JF, Maack DJ, Lin S. Purification and initial characterization of a protein from skeletal muscle that caps the barbed ends of actin filaments. J Biol Chem. 1986 Aug 15;261(23):10915–10921. [PubMed]
  • Cooper JA, Blum JD, Pollard TD. Acanthamoeba castellanii capping protein: properties, mechanism of action, immunologic cross-reactivity, and localization. J Cell Biol. 1984 Jul;99(1 Pt 1):217–225. [PMC free article] [PubMed]
  • Craig SW, Powell LD. Regulation of actin polymerization by villin, a 95,000 dalton cytoskeletal component of intestinal brush borders. Cell. 1980 Dec;22(3):739–746. [PubMed]
  • Davis J, Bennett V. Brain spectrin. Isolation of subunits and formation of hybrids with erythrocyte spectrin subunits. J Biol Chem. 1983 Jun 25;258(12):7757–7766. [PubMed]
  • Dlugosz AA, Antin PB, Nachmias VT, Holtzer H. The relationship between stress fiber-like structures and nascent myofibrils in cultured cardiac myocytes. J Cell Biol. 1984 Dec;99(6):2268–2278. [PMC free article] [PubMed]
  • Eppenberger HM, Strehler E. Preparation and properties of M-line proteins. Methods Enzymol. 1982;85(Pt B):139–149. [PubMed]
  • Fuseler JW, Shay JW. The association of desmin with the developing myofibrils of cultured embryonic rat heart myocytes. Dev Biol. 1982 Jun;91(2):448–457. [PubMed]
  • Gard DL, Lazarides E. The synthesis and distribution of desmin and vimentin during myogenesis in vitro. Cell. 1980 Jan;19(1):263–275. [PubMed]
  • Giffard RG, Weeds AG, Spudich JA. Ca2+-dependent binding of severin to actin: a one-to-one complex is formed. J Cell Biol. 1984 May;98(5):1796–1803. [PMC free article] [PubMed]
  • Glenney JR, Jr, Kaulfus P, Weber K. F actin assembly modulated by villin: Ca++-dependent nucleation and capping of the barbed end. Cell. 1981 May;24(2):471–480. [PubMed]
  • Granger BL, Lazarides E. The existence of an insoluble Z disc scaffold in chicken skeletal muscle. Cell. 1978 Dec;15(4):1253–1268. [PubMed]
  • Hasegawa T, Takahashi S, Hayashi H, Hatano S. Fragmin: a calcium ion sensitive regulatory factor on the formation of actin filaments. Biochemistry. 1980 Jun 10;19(12):2677–2683. [PubMed]
  • Isenberg G, Aebi U, Pollard TD. An actin-binding protein from Acanthamoeba regulates actin filament polymerization and interactions. Nature. 1980 Dec 4;288(5790):455–459. [PubMed]
  • Kilimann MW, Isenberg G. Actin filament capping protein from bovine brain. EMBO J. 1982;1(7):889–894. [PMC free article] [PubMed]
  • Knight PJ, Trinick JA. Preparation of myofibrils. Methods Enzymol. 1982;85(Pt B):9–12. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Maher PA, Cox GF, Singer SJ. Zeugmatin: a new high molecular weight protein associated with Z lines in adult and early embryonic striated muscle. J Cell Biol. 1985 Nov;101(5 Pt 1):1871–1883. [PMC free article] [PubMed]
  • Muguruma M, Kobayashi K, Fukazawa T, Ohashi K, Maruyama K. A new 220,000 dalton protein located in the Z lines of vertebrate skeletal muscle. J Biochem. 1981 Jun;89(6):1981–1984. [PubMed]
  • Nelson WJ, Lazarides E. Expression of the beta subunit of spectrin in nonerythroid cells. Proc Natl Acad Sci U S A. 1983 Jan;80(2):363–367. [PMC free article] [PubMed]
  • Nunnally MH, Powell LD, Craig SW. Reconstitution and regulation of actin gel-sol transformation with purified filamin and villin. J Biol Chem. 1981 Mar 10;256(5):2083–2086. [PubMed]
  • Ohashi K, Maruyama K. A new structural protein located in the Z lines of chicken skeletal muscle. J Biochem. 1979 Apr;85(4):1103–1105. [PubMed]
  • Pollard TD, Cooper JA. Actin and actin-binding proteins. A critical evaluation of mechanisms and functions. Annu Rev Biochem. 1986;55:987–1035. [PubMed]
  • Roberts IM. Tungsten coating--a method of improving glass microtome knives for cutting ultrathin sections. J Microsc. 1975 Jan;103(1):113–119. [PubMed]
  • Rouayrenc JF, Fattoum A, Gabrion J, Audemard E, Kassab R. Muscle gelsolin: isolation from heart tissue and characterization as an integral myofibrillar protein. FEBS Lett. 1984 Feb 13;167(1):52–58. [PubMed]
  • Schleicher M, Gerisch G, Isenberg G. New actin-binding proteins from Dictyostelium discoideum. EMBO J. 1984 Sep;3(9):2095–2100. [PMC free article] [PubMed]
  • Stossel TP, Chaponnier C, Ezzell RM, Hartwig JH, Janmey PA, Kwiatkowski DJ, Lind SE, Smith DB, Southwick FS, Yin HL, et al. Nonmuscle actin-binding proteins. Annu Rev Cell Biol. 1985;1:353–402. [PubMed]
  • Stromer MH, Goll DE. Studies on purified -actinin. II. Electron microscopic studies on the competitive binding of -actinin and tropomyosin to Z-line extracted myofibrils. J Mol Biol. 1972 Jun 28;67(3):489–494. [PubMed]
  • Tokuyasu KT. Immunochemistry on ultrathin frozen sections. Histochem J. 1980 Jul;12(4):381–403. [PubMed]
  • Trinick J, Lowey S. M-protein from chicken pectoralis muscle: isolation and characterization. J Mol Biol. 1977 Jun 25;113(2):343–368. [PubMed]
  • Tseng PC, Pollard TD. Mechanism of action of Acanthamoeba profilin: demonstration of actin species specificity and regulation by micromolar concentrations of MgCl2. J Cell Biol. 1982 Jul;94(1):213–218. [PMC free article] [PubMed]
  • Wang E, Yin HL, Krueger JG, Caliguiri LA, Tamm I. Unphosphorylated gelsolin is localized in regions of cell-substratum contact or attachment in Rous sarcoma virus-transformed rat cells. J Cell Biol. 1984 Feb;98(2):761–771. [PMC free article] [PubMed]
  • Wilkins JA, Risinger MA, Lin S. Studies on proteins that co-purify with smooth muscle vinculin: identification of immunologically related species in focal adhesions of nonmuscle and Z-lines of muscle cells. J Cell Biol. 1986 Oct;103(4):1483–1494. [PMC free article] [PubMed]
  • Yamaguchi M, Izumimoto M, Robson RM, Stromer MH. Fine structure of wide and narrow vertebrate muscle Z-lines. A proposed model and computer simulation of Z-line architecture. J Mol Biol. 1985 Aug 20;184(4):621–643. [PubMed]
  • Yin HL, Albrecht JH, Fattoum A. Identification of gelsolin, a Ca2+-dependent regulatory protein of actin gel-sol transformation, and its intracellular distribution in a variety of cells and tissues. J Cell Biol. 1981 Dec;91(3 Pt 1):901–906. [PMC free article] [PubMed]
  • Yin HL, Zaner KS, Stossel TP. Ca2+ control of actin gelation. Interaction of gelsolin with actin filaments and regulation of actin gelation. J Biol Chem. 1980 Oct 10;255(19):9494–9500. [PubMed]

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