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J Cell Biol. 1987 Oct 1; 105(4): 1539–1547.
PMCID: PMC2114659

Multiple methods of visualizing the yeast vacuole permit evaluation of its morphology and inheritance during the cell cycle


The vacuole of the yeast Saccharomyces cerevisiae was visualized with three unrelated fluorescent dyes: FITC-dextran, quinacrine, and an endogenous fluorophore produced in ade2 yeast. FITC-dextran, which enters cells by endocytosis, had been previously developed as a vital stain for yeast vacuoles. Quinacrine, which diffuses across membranes and accumulates in acidic compartments in mammalian cells, can also be used as a marker for yeast vacuoles. ade2 yeast accumulate an endogenous fluorophore in their vacuoles. Using these stains, yeast were examined for vacuole morphology throughout the cell division cycle. In both the parent cell and the bud, a single vacuole was the most common morphology at every stage. Two or more vacuoles could also be found in the mother cell or in the bud; however, this morphology was not correlated with any stage of the cell division cycle. Even small buds (in early S phase) often contained a small vacuole. By the time the bud was half the diameter of the mother cell, it almost always bore a vacuole. This picture of vacuole division and segregation differs from what is seen with synchronized cultures. In ade2 yeast, the bud usually inherits a substantial portion of its vacuole contents from the mother cell. We propose that vacuolar segregation is accomplished by vesicular traffic between the parent cell and the bud.

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Selected References

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  • Ammerer G, Hunter CP, Rothman JH, Saari GC, Valls LA, Stevens TH. PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. Mol Cell Biol. 1986 Jul;6(7):2490–2499. [PMC free article] [PubMed]
  • Birky CW., Jr The partitioning of cytoplasmic organelles at cell division. Int Rev Cytol Suppl. 1983;15:49–89. [PubMed]
  • Dorfman BZ. The isolation of adenylosuccinate synthetase mutants in yeast by selection for constitutive behavior in pigmented strains. Genetics. 1969 Feb;61(2):377–389. [PMC free article] [PubMed]
  • Dunphy WG, Rothman JE. Compartmental organization of the Golgi stack. Cell. 1985 Aug;42(1):13–21. [PubMed]
  • Jones EW. The synthesis and function of proteases in Saccharomyces: genetic approaches. Annu Rev Genet. 1984;18:233–270. [PubMed]
  • Jones EW, Zubenko GS, Parker RR. PEP4 gene function is required for expression of several vacuolar hydrolases in Saccharomyces cerevisiae. Genetics. 1982 Dec;102(4):665–677. [PMC free article] [PubMed]
  • Lazarow PB, Fujiki Y. Biogenesis of peroxisomes. Annu Rev Cell Biol. 1985;1:489–530. [PubMed]
  • Makarow M. Endocytosis in Saccharomyces cerevisiae: internalization of alpha-amylase and fluorescent dextran into cells. EMBO J. 1985 Jul;4(7):1861–1866. [PMC free article] [PubMed]
  • Makarow M, Nevalainen LT. Transport of a fluorescent macromolecule via endosomes to the vacuole in Saccharomyces cerevisiae. J Cell Biol. 1987 Jan;104(1):67–75. [PMC free article] [PubMed]
  • Messenguy F, Colin D, ten Have JP. Regulation of compartmentation of amino acid pools in Saccharomyces cerevisiae and its effects on metabolic control. Eur J Biochem. 1980 Jul;108(2):439–447. [PubMed]
  • Poole B, Ohkuma S. Effect of weak bases on the intralysosomal pH in mouse peritoneal macrophages. J Cell Biol. 1981 Sep;90(3):665–669. [PMC free article] [PubMed]
  • Severs NJ, Jordan EG, Williamson DH. Nuclear pore absence from areas of close association between nucleus and vacuole in synchronous yeast cultures. J Ultrastruct Res. 1976 Mar;54(3):374–387. [PubMed]
  • Smirnov MN, Smirnov VN, Budowsky EI, Inge-Vechtomov SG, Serebrjakov NG. Red pigment of adenine-deficient yeast Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1967 May 5;27(3):299–304. [PubMed]
  • Stevens TH, Rothman JH, Payne GS, Schekman R. Gene dosage-dependent secretion of yeast vacuolar carboxypeptidase Y. J Cell Biol. 1986 May;102(5):1551–1557. [PMC free article] [PubMed]
  • SVIHLA G, SCHLENK F. S-adenosylmethionine in the vacuole of Candida utilis. J Bacteriol. 1960 Jun;79:841–848. [PMC free article] [PubMed]
  • Thomas JH, Botstein D. A gene required for the separation of chromosomes on the spindle apparatus in yeast. Cell. 1986 Jan 17;44(1):65–76. [PubMed]
  • Wiemken A. Isolation of vacuoles from yeasts. Methods Cell Biol. 1975;12:99–109. [PubMed]
  • Wiemken A, Matile P, Moor H. Vacuolar dynamics in synchronously budding yeast. Arch Mikrobiol. 1970;70(2):89–103. [PubMed]
  • Woolford CA, Daniels LB, Park FJ, Jones EW, Van Arsdell JN, Innis MA. The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases. Mol Cell Biol. 1986 Jul;6(7):2500–2510. [PMC free article] [PubMed]

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