• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Sep 1988; 170(9): 4136–4140.
PMCID: PMC211419

RNA polymerase subunit homology among cyanobacteria, other eubacteria and archaebacteria.

Abstract

RNA polymerase purified from vegetative cells of the cyanobacterium Anabaena sp. strain PCC 7120 contains a dissociable sigma factor and a core of five subunits: the beta', beta, and two alpha subunits characteristic of all eubacteria and an additional 66,000-molecular-weight polypeptide called gamma. Fifteen of fifteen strains of unicellular and filamentous cyanobacteria tested contained a serologically related gamma protein. Antiserum to gamma reacted with Escherichia coli beta' and the A subunit of RNA polymerase of the archaebacterium Sulfolobus acidocaldarius. Thus the evolution of the RNA polymerase beta' subunit has followed different paths in three groups of procaryotes: cyanobacteria, other eubacteria, and archaebacteria.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Burgess RR, Travers AA, Dunn JJ, Bautz EK. Factor stimulating transcription by RNA polymerase. Nature. 1969 Jan 4;221(5175):43–46. [PubMed]
  • Gierl A, Zillig W, Stetter KO. The role of the components sigma and y of the DNA-dependent RNA polymerase of Lactobacillus curvatus in promotor selection. Eur J Biochem. 1982 Jun 15;125(1):41–47. [PubMed]
  • Grachev MA, Kolocheva TI, Lukhtanov EA, Mustaev AA. Studies on the functional topography of Escherichia coli RNA polymerase. Highly selective affinity labelling by analogues of initiating substrates. Eur J Biochem. 1987 Feb 16;163(1):113–121. [PubMed]
  • Herzfeld F, Zillig W. Subunit composition of DNA-dependent RNA polymerase of Anacystis nidulans. Eur J Biochem. 1971 Dec;24(2):242–248. [PubMed]
  • Hudson GS, Holton TA, Whitfield PR, Bottomley W. Spinach chloroplast rpoBC genes encode three subunits of the chloroplast RNA polymerase. J Mol Biol. 1988 Apr 20;200(4):639–654. [PubMed]
  • Kidd GH, Bogorad L. A facile procedure for purifying maize chloroplast RNA polymerase from whole cell homogenates. Biochim Biophys Acta. 1980 Aug 26;609(1):14–30. [PubMed]
  • Kranz RG, Gennis RB. A quantitative radioimmunological screening method for specific gene products. Anal Biochem. 1982 Dec;127(2):247–257. [PubMed]
  • Lerbs S, Bräutigam E, Parthier B. Polypeptides of DNA-dependent RNA polymerase of spinach chloroplasts: characterization by antibody-linked polymerase assay and determination of sites of synthesis. EMBO J. 1985 Jul;4(7):1661–1666. [PMC free article] [PubMed]
  • Miller SS, Bogorad L. Purification and Characterization of RNA Polymerase from Fremyella diplosiphon. Plant Physiol. 1978 Dec;62(6):995–999. [PMC free article] [PubMed]
  • Nierzwicki-Bauer SA, Haselkorn R. Differences in mRNA levels in Anabaena living freely or in symbiotic association with Azolla. EMBO J. 1986 Jan;5(1):29–35. [PMC free article] [PubMed]
  • Orr J, Keefer LM, Keim P, Nguyen TD, Wellems T, Heinrikson RL, Haselkorn R. Purification, physical characterization, and NH2-terminal sequence of glutamine synthetase from the cyanobacterium Anabaena 7120. J Biol Chem. 1981 Dec 25;256(24):13091–13098. [PubMed]
  • Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, et al. The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J. 1986 Sep;5(9):2043–2049. [PMC free article] [PubMed]
  • Smith HJ, Bogorad L. The polypeptide subunit structure of the DNA-dependent RNA polymerase of Zea mays chloroplasts. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4839–4842. [PMC free article] [PubMed]
  • Studier FW. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. [PubMed]
  • Woese CR. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...