Logo of jcellbiolHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Cell Biol. 1985 Feb 1; 100(2): 364–374.
PMCID: PMC2113439

Interaction of the 70,000-mol-wt amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts


Plasma fibronectin binds saturably and reversibly to substrate-attached fibroblasts and is subsequently incorporated into the extracellular matrix (McKeown-Longo, P.J., and D. F. Mosher, 1983, J. Cell Biol., 97:466-472). We examined whether fragments of fibronectin are processed in a similar way. The amino-terminal 70,000-mol-wt catheptic D fragment of fibronectin bound reversibly to cell surfaces with the same affinity as intact fibronectin but did not become incorporated into extracellular matrix. The 70,000-mol-wt fragment blocked binding of intact fibronectin to cell surfaces and incorporation of intact fibronectin into extracellular matrix. Binding of the 70,000-mol-wt fragment to cells was partially abolished by cleavage into 27,000-mol- wt heparin-binding and 40,000-mol-wt gelatin-binding fragments and more completely abolished by reduction and alkylation of disulfide bonds. Binding of the 70,000-mol-wt fragment to cells was not blocked by gelatin or heparin. When coated onto plastic, the 70,000-mol-wt fragment did not mediate attachment and spreading of suspended fibroblasts. Conversely, fibronectin fragments that had attachment and spreading activity did not block binding of exogenous fibronectin to substrate-attached cells. These results indicate that there is a cell binding site in the 70,000-mol-wt fragment that is distinct from the previously described cell attachment site and is required for assembly of exogenous fibronectin into extracellular matrix.

Full Text

The Full Text of this article is available as a PDF (1.2M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aplin JD, Hughes RC, Jaffe CL, Sharon N. Reversible cross-linking of cellular components of adherent fibroblasts to fibronectin and lectin-coated substrata. Exp Cell Res. 1981 Aug;134(2):488–494. [PubMed]
  • Atherton BT, Hynes RO. A difference between plasma and cellular fibronectins located with monoclonal antibodies. Cell. 1981 Jul;25(1):133–141. [PubMed]
  • Balian G, Click EM, Crouch E, Davidson JM, Bornstein P. Isolation of a collagen-binding fragment from fibronectin and cold-insoluble globulin. J Biol Chem. 1979 Mar 10;254(5):1429–1432. [PubMed]
  • Carter WG, Hakomori S. A new cell surface, detergent-insoluble glycoprotein matrix of human and hamster fibroblasts. The role of disulfide bonds in stabilization of the matrix. J Biol Chem. 1981 Jul 10;256(13):6953–6960. [PubMed]
  • Chen LB, Gallimore PH, McDougall JK. Correlation between tumor induction and the large external transformation sensitive protein on the cell surface. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3570–3574. [PMC free article] [PubMed]
  • Chen LB, Murray A, Segal RA, Bushnell A, Walsh ML. Studies on intercellular LETS glycoprotein matrices. Cell. 1978 Jun;14(2):377–391. [PubMed]
  • Choi MG, Hynes RO. Biosynthesis and processing of fibronectin in NIL.8 hamster cells. J Biol Chem. 1979 Dec 10;254(23):12050–12055. [PubMed]
  • De Petro G, Barlati S, Vartio T, Vaheri A. Transformation-enhancing activity of gelatin-binding fragments of fibronectin. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4965–4969. [PMC free article] [PubMed]
  • Ehrismann R, Chiquet M, Turner DC. Mode of action of fibronectin in promoting chicken myoblast attachment. Mr = 60,000 gelatin-binding fragment binds native fibronectin. J Biol Chem. 1981 Apr 25;256(8):4056–4062. [PubMed]
  • Ehrismann R, Roth DE, Eppenberger HM, Turner DC. Arrangement of attachment-promoting, self-association, and heparin-binding sites in horse serum fibronectin. J Biol Chem. 1982 Jul 10;257(13):7381–7387. [PubMed]
  • Furcht LT, Smith D, Wendelschafer-Crabb G, Mosher DF, Foidart JM. Fibronectin presence in native collagen fibrils of human fibroblasts: immunoperoxidase and immunoferritin localization. J Histochem Cytochem. 1980 Dec;28(12):1319–1333. [PubMed]
  • Grinnell F, Hays DG, Minter D. Cell adhesion and spreading factor. Partial purification and properties. Exp Cell Res. 1977 Nov;110(1):175–190. [PubMed]
  • Hahn LH, Yamada KM. Isolation and biological characterization of active fragments of the adhesive glycoprotein fibronectin. Cell. 1979 Dec;18(4):1043–1051. [PubMed]
  • Hayashi M, Yamada KM. Differences in domain structures between plasma and cellular fibronectins. J Biol Chem. 1981 Nov 10;256(21):11292–11300. [PubMed]
  • Hayashi M, Yamada KM. Domain structure of the carboxyl-terminal half of human plasma fibronectin. J Biol Chem. 1983 Mar 10;258(5):3332–3340. [PubMed]
  • Hayman EG, Ruoslahti E. Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix. J Cell Biol. 1979 Oct;83(1):255–259. [PMC free article] [PubMed]
  • Hedman K, Kurkinen M, Alitalo K, Vaheri A, Johansson S, Hök M. Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol. 1979 Apr;81(1):83–91. [PMC free article] [PubMed]
  • Humphries MJ, Ayad SR. Stimulation of DNA synthesis by cathepsin D digests of fibronectin. Nature. 305(5937):811–813. [PubMed]
  • Hynes RO, Destree A. Extensive disulfide bonding at the mammalian cell surface. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2855–2859. [PMC free article] [PubMed]
  • Hynes RO, Yamada KM. Fibronectins: multifunctional modular glycoproteins. J Cell Biol. 1982 Nov;95(2 Pt 1):369–377. [PMC free article] [PubMed]
  • Irish PS, Hasty DL. Immunocytochemical localization of fibronectin in human fibroblast cultures using a cell surface replica technique. J Histochem Cytochem. 1983 Jan;31(1):69–77. [PubMed]
  • Johansson S, Hök M. Substrate adhesion of rat hepatocytes: on the mechanism of attachment to fibronectin. J Cell Biol. 1984 Mar;98(3):810–817. [PMC free article] [PubMed]
  • Keski-Oja J, Mosher DF, Vaheri A. Dimeric character of fibronectin, a major cell surface-associated glycoprotein. Biochem Biophys Res Commun. 1977 Jan 24;74(2):699–706. [PubMed]
  • Kleinman HK, McGoodwin EB. Localization of the cell attachment region in types I and II collagens. Biochem Biophys Res Commun. 1976 Sep 20;72(2):426–432. [PubMed]
  • Kleinman HK, Martin GR, Fishman PH. Ganglioside inhibition of fibronectin-mediated cell adhesion to collagen. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3367–3371. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Maciag T, Kadish J, Wilkins L, Stemerman MB, Weinstein R. Organizational behavior of human umbilical vein endothelial cells. J Cell Biol. 1982 Sep;94(3):511–520. [PMC free article] [PubMed]
  • McAbee DD, Grinnell F. Fibronectin-mediated binding and phagocytosis of polystyrene latex beads by baby hamster kidney cells. J Cell Biol. 1983 Nov;97(5 Pt 1):1515–1523. [PMC free article] [PubMed]
  • McConnell MR, Blumberg PM, Rossow PW. Dimeric and high molecular weight forms of the large external transformation-sensitive protein on the surface of chick embryo fibroblasts. J Biol Chem. 1978 Oct 25;253(20):7522–7530. [PubMed]
  • McKeown-Longo PJ, Mosher DF. Binding of plasma fibronectin to cell layers of human skin fibroblasts. J Cell Biol. 1983 Aug;97(2):466–472. [PMC free article] [PubMed]
  • McKeown-Longo PJ, Mosher DF. Mechanism of formation of disulfide-bonded multimers of plasma fibronectin in cell layers of cultured human fibroblasts. J Biol Chem. 1984 Oct 10;259(19):12210–12215. [PubMed]
  • Mosher DF. Fibronectin. Prog Hemost Thromb. 1980;5:111–151. [PubMed]
  • Mosher DF, Proctor RA. Binding and factor XIIIa-mediated cross-linking of a 27-kilodalton fragment of fibronectin to Staphylococcus aureus. Science. 1980 Aug 22;209(4459):927–929. [PubMed]
  • Mosher DF, Johnson RB. In vitro formation of disulfide-bonded fibronectin multimers. J Biol Chem. 1983 May 25;258(10):6595–6601. [PubMed]
  • Mosher DF, Schad PE, Vann JM. Cross-linking of collagen and fibronectin by factor XIIIa. Localization of participating glutaminyl residues to a tryptic fragment of fibronectin. J Biol Chem. 1980 Feb 10;255(3):1181–1188. [PubMed]
  • Oh E, Pierschbacher M, Ruoslahti E. Deposition of plasma fibronectin in tissues. Proc Natl Acad Sci U S A. 1981 May;78(5):3218–3221. [PMC free article] [PubMed]
  • Oppenheimer-Marks N, Grinnell F. Inhibition of fibronectin receptor function by antibodies against baby hamster kidney cell wheat germ agglutinin receptors. J Cell Biol. 1982 Dec;95(3):876–884. [PMC free article] [PubMed]
  • Pena SD, Hughes RC. Fibronectin-plasma membrane interactions in the adhesion and spreading of hamster fibroblasts. Nature. 1978 Nov 2;276(5683):80–83. [PubMed]
  • Petersen TE, Thøgersen HC, Skorstengaard K, Vibe-Pedersen K, Sahl P, Sottrup-Jensen L, Magnusson S. Partial primary structure of bovine plasma fibronectin: three types of internal homology. Proc Natl Acad Sci U S A. 1983 Jan;80(1):137–141. [PMC free article] [PubMed]
  • Pierschbacher MD, Hayman EG, Ruoslahti E. Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule. Cell. 1981 Oct;26(2 Pt 2):259–267. [PubMed]
  • Rennard SI, Wind ML, Hewitt AT, Kleinman HK. Effect of collagen and cell shape on binding of fibronectin to cells. Arch Biochem Biophys. 1981 Jan;206(1):205–212. [PubMed]
  • Richter H, Seidl M, Hörmann H. Location of heparin-binding sites of fibronectin. Detection of a hitherto unrecognized transamidase sensitive site. Hoppe Seylers Z Physiol Chem. 1981 Apr;362(4):399–408. [PubMed]
  • Ruoslahti E, Engvall E, Hayman EG. Fibronectin: current concepts of its structure and functions. Coll Relat Res. 1981;1(1):95–128. [PubMed]
  • Schwarzbauer JE, Tamkun JW, Lemischka IR, Hynes RO. Three different fibronectin mRNAs arise by alternative splicing within the coding region. Cell. 1983 Dec;35(2 Pt 1):421–431. [PubMed]
  • Sekiguchi K, Hakomori S. Domain structure of human plasma fibronectin. Differences and similarities between human and hamster fibronectins. J Biol Chem. 1983 Mar 25;258(6):3967–3973. [PubMed]
  • Singer II. The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts. Cell. 1979 Mar;16(3):675–685. [PubMed]
  • Singer II, Kawka DW, Kazazis DM, Clark RA. In vivo co-distribution of fibronectin and actin fibers in granulation tissue: immunofluorescence and electron microscope studies of the fibronexus at the myofibroblast surface. J Cell Biol. 1984 Jun;98(6):2091–2106. [PMC free article] [PubMed]
  • Skorstengaard K, Thøgersen HC, Petersen TE. Complete primary structure of the collagen-binding domain of bovine fibronectin. Eur J Biochem. 1984 Apr 16;140(2):235–243. [PubMed]
  • Smith DE, Mosher DF, Johnson RB, Furcht LT. Immunological identification of two sulfhydryl-containing fragments of human plasma fibronectin. J Biol Chem. 1982 May 25;257(10):5831–5838. [PubMed]
  • Tarone G, Galetto G, Prat M, Comoglio PM. Cell surface molecules and fibronectin-mediated cell adhesion: effect of proteolytic digestion of membrane proteins. J Cell Biol. 1982 Jul;94(1):179–186. [PMC free article] [PubMed]
  • Vibe-Pedersen K, Sahl P, Skorstengaard K, Petersen TE. Amino acid sequence of a peptide from bovine plasma fibronectin containing a free sulfhydryl group (cysteine). FEBS Lett. 1982 Jun 1;142(1):27–30. [PubMed]
  • Wagner DD, Hynes RO. Topological arrangement of the major structural features of fibronectin. J Biol Chem. 1980 May 10;255(9):4304–4312. [PubMed]
  • Williams EC, Janmey PA, Ferry JD, Mosher DF. Conformational states of fibronectin. Effects of pH, ionic strength, and collagen binding. J Biol Chem. 1982 Dec 25;257(24):14973–14978. [PubMed]
  • Williams EC, Janmey PA, Johnson RB, Mosher DF. Fibronectin. Effect of disulfide bond reduction on its physical and functional properties. J Biol Chem. 1983 May 10;258(9):5911–5914. [PubMed]
  • Yamada KM, Critchley DR, Fishman PH, Moss J. Exogenous gangliosides enhance the interaction of fibronectin with ganglioside-deficient cells. Exp Cell Res. 1983 Feb;143(2):295–302. [PubMed]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Cited in Books
    Cited in Books
    NCBI Bookshelf books that cite the current articles.
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...