• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. Jul 1988; 170(7): 3249–3254.
PMCID: PMC211277

Physiology of behavioral mutants of Rhizobium meliloti: evidence for a dual chemotaxis pathway.

Abstract

Wild-type and nonchemotactic mutant strains of Rhizobium meliloti were tested for attraction to localized sites on alfalfa roots and for attraction to numerous small molecules, including sugars, amino acids, and two fractions derived from alfalfa root extracts. Four strains (carrying mutations che-6, che-11, che-12, and che-26) lost all responses and were classified as generally nonchemotactic mutants. One strain (carrying mutation che-7) lost responses to a group of structurally unrelated amino acids but retained all other responses and was classified as a putative sensory transducer mutant. Two strains (carrying mutations che-1 and che-3) lost responses to all the amino acids and sugars tested but retained normal responses to localized sites on roots and to the root fractions. These two mutant strains could not be classified according to the generally accepted model for a sensory pathway, derived from studies of enteric bacteria, and provided evidence for a dual chemotaxis pathway in R. meliloti.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J Gen Microbiol. 1973 Jan;74(1):77–91. [PubMed]
  • Ames P, Bergman K. Competitive advantage provided by bacterial motility in the formation of nodules by Rhizobium meliloti. J Bacteriol. 1981 Nov;148(2):728–p. [PMC free article] [PubMed]
  • Ames P, Schluederberg SA, Bergman K. Behavioral mutants of Rhizobium meliloti. J Bacteriol. 1980 Feb;141(2):722–727. [PMC free article] [PubMed]
  • Block SM, Segall JE, Berg HC. Impulse responses in bacterial chemotaxis. Cell. 1982 Nov;31(1):215–226. [PubMed]
  • Boyd A, Simon M. Bacterial chemotaxis. Annu Rev Physiol. 1982;44:501–517. [PubMed]
  • Caetano-Anollés G, Wall LG, De Micheli AT, Macchi EM, Bauer WD, Favelukes G. Role of Motility and Chemotaxis in Efficiency of Nodulation by Rhizobium meliloti. Plant Physiol. 1988 Apr;86(4):1228–1235. [PMC free article] [PubMed]
  • Finan TM, Hartweig E, LeMieux K, Bergman K, Walker GC, Signer ER. General transduction in Rhizobium meliloti. J Bacteriol. 1984 Jul;159(1):120–124. [PMC free article] [PubMed]
  • Gulash M, Ames P, Larosiliere RC, Bergman K. Rhizobia are attracted to localized sites on legume roots. Appl Environ Microbiol. 1984 Jul;48(1):149–152. [PMC free article] [PubMed]
  • Hayashi T, Tsuchiya H, Naruse H. Reversed-phase ion-pair chromatography of amino acids. Application to the determination of amino acids in plasma samples and dried blood on filter papers. J Chromatogr. 1983 May 13;274:318–324. [PubMed]
  • Hedblom ML, Adler J. Chemotactic response of Escherichia coli to chemically synthesized amino acids. J Bacteriol. 1983 Sep;155(3):1463–1466. [PMC free article] [PubMed]
  • Langer R, Fefferman M, Gryska P, Bergman K. A simple method for studying chemotaxis using sustained release of attractants from inert polymers. Can J Microbiol. 1980 Feb;26(2):274–278. [PubMed]
  • Leigh JA, Signer ER, Walker GC. Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6231–6235. [PMC free article] [PubMed]
  • Napoli C, Albersheim P. Infection and nodulation of clover by nonmotile Rhizobium trifolii. J Bacteriol. 1980 Feb;141(2):979–980. [PMC free article] [PubMed]
  • Niwano M, Taylor BL. Novel sensory adaptation mechanism in bacterial chemotaxis to oxygen and phosphotransferase substrates. Proc Natl Acad Sci U S A. 1982 Jan;79(1):11–15. [PMC free article] [PubMed]
  • Spudich JL, Koshland DE., Jr Quantitation of the sensory response in bacterial chemotaxis. Proc Natl Acad Sci U S A. 1975 Feb;72(2):710–713. [PMC free article] [PubMed]
  • Vesper SJ, Bauer WD. Role of Pili (Fimbriae) in Attachment of Bradyrhizobium japonicum to Soybean Roots. Appl Environ Microbiol. 1986 Jul;52(1):134–141. [PMC free article] [PubMed]
  • Ziegler RJ, Peirce C, Bergman K. Mapping and cloning of a fla-che region of the Rhizobium meliloti chromosome. J Bacteriol. 1986 Nov;168(2):785–790. [PMC free article] [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...