Logo of jcellbiolHomeThe Rockefeller University PressEditorsContactInstructions for AuthorsThis issue
J Cell Biol. Aug 1, 1981; 90(2): 507–514.
PMCID: PMC2111870

Demonstration of contractility of circumferential actin bundles and its morphogenetic significance in pigmented epithelium in vitro and in vivo

Abstract

Each pigmented epithelial cell bears circumferential actin bundles at its apical level when the pigmented epithelium is established in eyes in situ or in culture in vitro. Well-differentiated pigmented epithelia in culture were treated with a 50% glycerol solution containing 0.1 M KCl, 5 mM EDTA, and 10 mM sodium phosphate buffer, pH 7.2, for 24 h or more at 4 degrees C. When the glycerinated epithelium was transferred to the ATP solution, each cell constituting the epithelium began to contract. The epithelium was cleaved into many cell groups as a result of contraction of each cell. The periphery of each cell group was lifted to form a cup or vesicle and eventually detached from the substratum. However, those cells that had not adhered tightly and not formed a monolayer epithelium with typical polygonal cellular pattern contracted independently as observed in the glycerinated fibroblasts. Contraction of the glycerinated pigmented epithelial cells was inhibited by N-ethylmaleimide but not by cytochalasin B. ITP and UTP also effected the contraction of the glycerinated cells, but GTP and ADP did not. Ca2+ was not required. This contractile model of pigmented epithelium provides a useful experimental system for analyzing the function of actin in cellular morphogenesis.

Full Text

The Full Text of this article is available as a PDF (1.1M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Baker PC, Schroeder TE. Cytoplasmic filaments and morphogenetic movement in the amphibian neural tube. Dev Biol. 1967 May;15(5):432–450. [PubMed]
  • Clarke M, Spudich JA. Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination. Annu Rev Biochem. 1977;46:797–822. [PubMed]
  • Crawford BJ. The structure and development of the pigmented retinal clone. Can J Zool. 1975 May;53(5):560–570. [PubMed]
  • Crawford B. Cloned pigmented retinal epiehtlium. The role of microfilaments in the differentiation of cell shape. J Cell Biol. 1979 May;81(2):301–315. [PMC free article] [PubMed]
  • Crawford B, Cloney RA, Cahn RD. Cloned pigmented retinal cells; the affects of cytochalasin B on ultrastructure and behavior. Z Zellforsch Mikrosk Anat. 1972;130(2):135–151. [PubMed]
  • Hitchcock SE. Regulation of motility in nonmuscle cells. J Cell Biol. 1977 Jul;74(1):1–15. [PMC free article] [PubMed]
  • HOFFMANN-BERLING H. Adenosintriphosphat als Betriebsstoff von Zellbewegungen. Biochim Biophys Acta. 1954 Jun;14(2):182–194. [PubMed]
  • Honda H, Eguchi G. How much does the cell boundary contract in a monolayered cell sheet? J Theor Biol. 1980 Jun 7;84(3):575–588. [PubMed]
  • Hsu LS, Becker EL. Volume decrease of glycerinated polymorphoneclear leukocytes induced by ATP and Ca-2. Exp Cell Res. 1975 Mar 15;91(2):469–473. [PubMed]
  • Isenberg G, Rathke PC, Hülsmann N, Franke WW, Wohlfarth-Bottermann KE. Cytoplasmic actomyosin fibrils in tissue culture cells: direct proof of contractility by visualization of ATP-induced contraction in fibrils isolated by laser micro-beam dissection. Cell Tissue Res. 1976 Feb 27;166(4):427–443. [PubMed]
  • Izzard CS, Izzard SL. Calcium regulation of the contractile state of isolated mammalian fibroblast cytoplasm. J Cell Sci. 1975 Jul;18(2):241–256. [PubMed]
  • Karfunkel P. The role of microtubules and microfilaments in neurulation in Xenopus. Dev Biol. 1971 May;25(1):30–56. [PubMed]
  • Korn ED. Biochemistry of actomyosin-dependent cell motility (a review). Proc Natl Acad Sci U S A. 1978 Feb;75(2):588–599. [PMC free article] [PubMed]
  • Mooseker MS. Brush border motility. Microvillar contraction in triton-treated brush borders isolated from intestinal epithelium. J Cell Biol. 1976 Nov;71(2):417–433. [PMC free article] [PubMed]
  • Owaribe K, Hatano S. Inducation of antibody against actin from myxomycete plasmodium and its properties. Biochemistry. 1975 Jul;14(13):3024–3029. [PubMed]
  • Pollard TD, Weihing RR. Actin and myosin and cell movement. CRC Crit Rev Biochem. 1974 Jan;2(1):1–65. [PubMed]
  • Rodewald R, Newman SB, Karnovsky MJ. Contraction of isolated brush borders from the intestinal epithelium. J Cell Biol. 1976 Sep;70(3):541–554. [PMC free article] [PubMed]
  • Shibata-sekiya K, Tonomura Y. Desensitization of substrate inhibition of acto-H-meromyosin ATPase by treatment of H-meromyosin with rho-chloromercuribenzoate. Relation between the extent of desensitization and the amount of bound rho-chloromercuribenzoate1. J Biochem. 1975 Mar;77(3):543–557. [PubMed]
  • Taylor DL, Condeelis JS, Moore PL, Allen RD. The contractile basis of amoeboid movement. I. The chemical control of motility in isolated cytoplasm. J Cell Biol. 1973 Nov;59(2 Pt 1):378–394. [PMC free article] [PubMed]
  • TONOMURA Y, YOSHIMURA J. Binding of p-chloromercuri-benzoate to actin. J Biochem. 1962 Apr;51:259–266. [PubMed]
  • Weber K, Rathke PC, Osborn M, Franke WW. Distribution of actin and tubulin in cells and in glycerinated cell models after treatment with cytochalasin B (CB). Exp Cell Res. 1976 Oct 15;102(2):285–297. [PubMed]
  • Wessells NK, Evans J. Ultrastructural studies of early morphogenesis and cytodifferentiation in the embryonic mammalian pancreas. Dev Biol. 1968 Apr;17(4):413–446. [PubMed]
  • Wessells NK, Spooner BS, Ash JF, Bradley MO, Luduena MA, Taylor EL, Wrenn JT, Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. [PubMed]
  • Wrenn JT, Wessells NK. Cytochalasin B: effects upon microfilaments involved in morphogenesis of estrogen-induced glands of oviduct. Proc Natl Acad Sci U S A. 1970 Jul;66(3):904–908. [PMC free article] [PubMed]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

Formats:

Related citations in PubMed

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...